Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

Pinho, Salomé S.; Matos, Augusto de; Lopes, Célia; Marcos, Nuno T.; Carvalheira, J.; Reis, Celso A.; Gärtner, Fátima

doi:10.1186/1471-2407-7-124

Cited by 32 publications

(36 citation statements)

References 44 publications

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“…34 In dogs, recent reports have confirmed that sialyl Lewis carbohydrates are detectable in canine malignant mammary tumors and that elevated levels of sLe x expression in these cancers are associated with lymph node metastases. 21,27 To our knowledge, this study is the first report of the detection of C2-O-sLe x carbohydrates in canine gastric carcinomas.…”

Section: Discussionmentioning

confidence: 99%

“…Tissues were counterstained with Mayer's hematoxylin. Incubations with protein block and primary and secondary antibodies were carried out in a humidified chamber at 27 C to 30 C. The remaining staining procedures were performed at room temperature. For comparison with the tumor tissues, samples of stomach from 5 normal dogs were processed and stained similarly using the CHO-131 and Lu-5 antibodies.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…21,23,27,28 In humans, the sLe x antigen on leukocytes functions as a ligand for the selectin family of cell adhesion molecules and mediates the binding of leukocytes to vascular endothelium at sites of inflammation. These selectin-binding interactions are greatly enhanced when leukocytes display sLe x on a core 2 branched O-glycan carbohydrate structure (C2-O-sLe x ).…”

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confidence: 99%

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The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

2010

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Sialyl Lewis x-modified core 2 branched O-glycans (C2-O-sLe x ) on human leukocytes mediate much higher-affinity adhesion to selectins on activated vascular endothelium than does sialyl Lewis x on other structures. In some canine and human carcinomas, high expression of sLe x -decorated carbohydrates has been associated with metastasis and, in humans, a poor prognosis, but detection in canine gastric carcinomas is unreported. The authors hypothesized that these carbohydrates are highly expressed in more malignant types of canine gastric carcinomas, they promote metastasis, and they are associated with a poorer prognosis for dogs. The objectives were to determine the presence and importance of C2-O-sLe x expression in canine gastric carcinomas. Routine histological sections of 16 canine gastric carcinomas were categorized on the basis of 3 classification schemes: World Health Organization, Lauren, and Goseki. Serial sections were stained with antibodies directed against C2-O-sLe x (CHO-131 monoclonal antibody), cytokeratin (Lu-5 monoclonal antibody), and stains to detect neutral and acid mucins (periodic acid-Schiff and alcian blue). Whereas normal gastric mucosal epithelial cells were negative for C2-O-sLe x , 56% of the tumors examined were positive for C2-O-sLe x . Importantly, the majority of more poorly differentiated tumor types had more numerous and larger intensely stained areas of C2-O-sLe x expression compared with moderate to well-differentiated tumor types. Signet ring-type carcinomas had markedly higher distribution and intensity of periodic acid-Schiff and alcian blue staining than did tubular carcinomas. These findings suggest that C2-O-sLe x is a tumor-associated antigen that may play a role in the invasiveness and metastatic potential of certain types of canine gastric carcinomas.

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

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confidence: 99%

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The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

2010

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“…Em pesquisa realizada com carcinomas mamários caninos, observou-se uma relação indireta da expressão das E-caderinas com a de Sialyl-Lewis x (sLe x ), um carboidrato, considerado marcador tumoral, que tem sua expressão aumentada associada à progressão do tumor e desenvolvimento de metástases. Quando a expressão de E-caderinas estava aumentada, a de sLe x estava diminuída, e vice-cersa (PINHO et al, 2007).…”

Section: Caderinas Como Indicadores De Prognósticounclassified

Expressão Das Caderinas Nos Tumores Mamários Em Cadelas

Piekarz¹,

Biondo²,

Amorim³

et al. 2008

AVS

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RESUMO -As neoplasias mamárias são as que mais acometem as cadelas. O desenvolvimento do câncer pode levar à ocorrência de metástases que podem ser detectadas por marcadores protéicos. Um destes marcadores é a E-caderina, um membro da família das caderinas conhecida por desempenhar um papel importante na regulação da adesão intercelular em tecidos epiteliais. Estudos apontam que as E-caderinas podem funcionar como moléculas supressoras de tumor e de invasão. A atividade das caderinas é regulada por múltiplos mecanismos, incluindo a interação com outras proteínas como as cateninas. Nesta revisão os autores abordam a família das caderinas, incluindo sua função, fisiopatologia e potencial uso como marcadores de diagnóstico e prognóstico nas neoplasias mamárias na cadela. Palavras-Chave: caderina, imunoistoquímica, neoplasia mamária, cão.ABSTRACT -Mammary tumors are among the most common neoplasia of canine females. The occurrence of metastasis may be detected by proteic markers. Among them, exist the E-cadherin, a member of cadherin family known for its important role in the regulation of intercellular adhesion in epithelial tissues. Studies suggest that E-cadherin may function as a tumor and invasion suppressor molecule. Cadherin activity is regulated by multiple mechanisms, including interaction with other proteins such as catenins. In this review, the authors approach the cadherin family and other related adhesion proteins including its function, physiopathology and potential use as marker for diagnosis and prognosis of canine mammary tumors.

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“…However, the evaluation of LVI and BVI in colon cancer tissue samples by regular HE staining gives significant false positive and false negative results (Merchant et al, 1995;Kikuchi et al, 1995;Nakai et al, 1 , Shao-Yuan Wang 2 2004). Therefore, exploring specific markers for LVI and BVI has become a hot topic of recent years (Pinho et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Clinical Significance of Detecting Lymphatic and Blood Vessel Invasion in Stage II Colon Cancer Using Markers D2-40 and CD34 in Combination

Lai¹,

Zhou²,

Du³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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This research was conducted to compare differences in colon cancer lymphatic vessel invasion (LVI) with D2-40 antibody labeling and regular HE staining, blood vessel invasion (BVI) with CD34 antibody labeling and HE staining and to assess the possibility of using D2-40-LVI/CD34-BVI in combination for predicting stage II colon cancer prognosis and guiding adjuvant chemotherapy.Anti-D2-40 and anti-CD34 antibodies were applied to tissue samples of 220 cases of stage II colon cancer to label lymphatic vessels and small blood vessels, respectively. LVI and BVI were assessed and multivariate COX regression analysis was performed for associations with colon cancer prognosis. Regular HE staining proved unable to differentiate lymphatic vessels from blood vessels, while D2-40 selectively labeled lymphatic endothelial cell cytosol and CD34 was widely expressed in large and small blood vessels of tumors as well as normal tissues. Compared to regular HE staining, D2-40-labeling for LVI and CD34-labeling for BVI significantly increased positive rate (22.3% vs 10.0% for LVI, and 19.1% vs 9.1% for BVI). Multivariate analysis indicated that TNM stage, pathology tissue type, post-surgery adjuvant chemotherapy, D2-40-LVI, and CD34-BVI were independent factors affecting whole group colon cancer prognosis, while HE staining-BVI, HE staining-LVI were not significantly related. When CD34-BVI/D2-40-LVI were used in combination for detection, the risk of death for patients with two or one positive results was 5.003 times that in the LVI(-)&BVI(-) group (95% CI 2.365 -9.679). D2-40 antibody LVI labeling and CD34 antibody BVI labeling have higher specificity and accuracy than regular HE staining and can be used as molecular biological indicators for prognosis prediction and guidance of adjuvant chemotherapy for stage II colon cancer.

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Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

Cited by 32 publications

References 44 publications

The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

Expressão Das Caderinas Nos Tumores Mamários Em Cadelas

Clinical Significance of Detecting Lymphatic and Blood Vessel Invasion in Stage II Colon Cancer Using Markers D2-40 and CD34 in Combination

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Sialyl Lewis x expression in canine malignant mammary tumours: correlation with clinicopathological features and E-Cadherin expression

Cited by 32 publications

References 44 publications

The Novel Carbohydrate Tumor Antigen C2-O-sLexIs Upregulated in Canine Gastric Carcinomas

The Novel Carbohydrate Tumor Antigen C2-O-sLexIs Upregulated in Canine Gastric Carcinomas

Expressão Das Caderinas Nos Tumores Mamários Em Cadelas

Clinical Significance of Detecting Lymphatic and Blood Vessel Invasion in Stage II Colon Cancer Using Markers D2-40 and CD34 in Combination

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The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas

The Novel Carbohydrate Tumor Antigen C2-O-sLe^xIs Upregulated in Canine Gastric Carcinomas