Sialoglycoprotein Isolated from Eggs of Carassius auratus Ameliorates Osteoporosis: An Effect Associated with Regulation of the Wnt/β-Catenin Pathway in Rodents

Abstract

In the current study, ovariectomized (OVX) rats and the senescence-accelerated mouse strain P6 (SAMP6) were employed to establish models of postmenopausal osteoporosis and senile osteoporosis, respectively. The effects of treatment with sialoglycoprotein isolated from the eggs of Carassius auratus (Ca-SGP) on these two types of osteoporosis were investigated in vivo. Results showed that Ca-SGP significantly increased bone mineral density, ameliorated trabecular bone microstructure, and improved bone biomechanical properties in both OVX rats and SAMP6. The osteogenesis related Wnt/β-catenin pathway was targeted to study the underlying mechanism of Ca-SGP activity. In postmenopausal osteoporosis, Ca-SGP suppressed the activation of Wnt/β-catenin signal via down-regulating the expression of key genes including LRP5, β-catenin, and Runx2, suggesting that overactive osteogenesis was controlled by Ca-SGP. The bone formation was sharply weakened in senile osteoporosis, whereas Ca-SGP treatment promoted osteoblast activity by stimulating the Wnt/β-catenin signal. In conclusion, Ca-SGP ameliorated these two types of osteoporosis by normalizing bone anabolism.

Radix Salviae miltiorrhizae improves bone microstructure and strength through Wnt/β‐catenin and osteoprotegerin/receptor activator for nuclear factor‐κB ligand/cathepsin K signaling in ovariectomized rats

“…In contrast, phosphorylation of LRP6 and GSK3β helps with the stabilization of β‐catenin and subsequent osteoblastogenesis (Baron & Kneissel, ; Manolagas, ). Ovariectomy‐induced bone loss was partly through the inhibition of Wnt/β‐catenin pathway via increasing β‐catenin phosphorylation and degradation (F. Wang, Wang, et al, ; Xia et al, ). In addition, DKK1 prevents Wnt binding to the extracellular regions and accordingly inhibits Wnt signal transduction and its subsequent bone‐forming effect (Zhou et al, ).…”

Tuna Bone Powder Alleviates Glucocorticoid‐Induced Osteoporosis via Coregulation of the NF‐κB and Wnt/β‐Catenin Signaling Pathways and Modulation of Gut Microbiota Composition and Metabolism

“…Glucocorticoids are 21‐carbon steroid hormones and long‐term use of glucocorticoids may break the balance in bone remodeling and results in osteoporosis . Current therapies for osteoporosis treatment by inducing osteoblast activity such as parathyroid hormone treatment, or inhibiting osteoclast function such as bisphosphonates treatment, have some limitations . Due to the complex compositions of bioactive substances in natural products and their possible synergistic effects, natural product‐derived alternatives for osteoporosis therapy are becoming more and more popular.…”

Celastrol inhibits glucocorticoid‑induced osteoporosis in rat via the PI3K/AKT and Wnt signaling pathways

“…osteoblasts (6,19). In bones, the Wnt/β-catenin pathway is vital for osteogenic differentiation and β-catenin may bind to nuclear transcription factors after entering the nucleus, to regulate a variety of proteins associated with osteogenic differentiation (5). BMP-2, a subtype of the transforming growth factor-β superfamily, has an important role in bone formation and bone metabolic balance in adults (20).…”

“…Under the action of the Wnt signaling pathway, mesenchymal stem cells differentiate into osteoblasts (5). The classic Wnt/β-catenin signal pathway in osteoblasts also regulates the formation of osteoclasts (6).…”