Sialidase and malignancy: A minireview

Miyagi, Taeko; Wada, Tadashi; Yamaguchi, Kazunori; Hata, Keiko

doi:10.1023/b:glyc.0000024250.48506.bf

Cited by 138 publications

(103 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To understand the causes and consequences of such aberrant sialylation, our studies have been focused on mammalian sialidases, which regulate cell-surface sialylation by removal of sialic acids from the nonreducing terminus of glycoproteins and glycolipids. Sialidase expression levels do indeed change in response to various cellular phenomena and especially in relation to cancer phenotype (Miyagi et al, 2004(Miyagi et al, , 2008. Four types of mammalian sialidases have been cloned to date (designated Neu1, Neu2, Neu3 and Neu4) differing in their subcellular localizations and substrate specificities (Monti et al, 2002;Miyagi and Yamaguchi, 2007).…”

Section: Introductionmentioning

confidence: 99%

Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin β4

et al. 2009

View full text Add to dashboard Cite

We previously found an inverse relationship between sialidase Neu1 expression and metastatic potential of murine cancer cells. To elucidate the mechanism underlying the cellular events, the human sialidase gene NEU1 was overexpressed or silenced in colon cancer HT-29 cells. When NEU1-overexpressing cells were injected transsplenically into mice, in vivo liver metastasis was significantly reduced. NEU1 suppressed cell migration, invasion and adhesion in vitro, whereas the silencing resulted in the opposite. One of the major molecular changes by NEU1 was decreased sialylation of integrin b4, assessed by PNAand MAL-II-lectin blotting of immunoprecipitates with anti-integrin b4 antibody. The desialylation was accompanied by decreased phosphorylation of the integrin followed by attenuation of focal adhesion kinase and Erk1/2 pathway. Moreover, NEU1 caused downregulation of matrix metalloproteinase-7, overexpression of which is associated with cancer metastasis. Treatment of the cells with GalNAc-a-O-benzyl, an inhibitor of O-glycosylation, showed increased PNA-positive integrin b4 with its decreased phosphorylation, indicating that sialic acid removal from the integrin O-glycans results in the decreased phosphorylation. Biotinylation and immunofluorescence staining exhibited some NEU1 molecules to be at the cell surface accessible to the integrin. These results suggest that NEU1 is important in regulation of integrin b4-mediated signaling, leading to suppression of metastasis.

show abstract

Section: Introductionmentioning

confidence: 99%

Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin β4

et al. 2009

View full text Add to dashboard Cite

show abstract

“…(3,17). They also point to possible changes in the activity/ expression of enzymes involved in glycosylation processes, both glycosyltransferases and glycosidases (18,19). In general, the glycophenotype of PCa is, among other changes, associated with an increase of sialic acid in cancerous tissue compared to normal tissue and may differentiate clearly between tumor cell sublines (20,21).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Pattern of Human Serum Glycoproteins in Prostate Cancer

Kosanović¹,

Janković²

2009

Journal of Medical Biochemistry

View full text Add to dashboard Cite

Kratak sadr`aj: Glikoproteomska istra`ivanja na nivou }e -lija, tkiva i biolo{kih te~nosti imaju za cilj otkrivanje novih biomarkera kao i strukturnih promena ve} poznatih biomarkera, koje su specifi~ne za kancer. U ovom radu je izvr{ena komparativna evaluacija glikoproteinskog profila normalnog humanog seruma i seruma pacijenata sa kance rom prostate (PCa), u smislu sijalinizacije i fuko zilacije, kao strukturnih promena relevantnih za progresiju kancera. Odgovaraju}i glikoproteini su izolovani lektinskom afini tetnom hromatografijom na kolonama sa imobilisanim Sambucus nigra aglutininom i Lens culinaris aglutininom i okarakterisani tehnikama SDS-PAGE i vezivanja za proteinski ~ip sa normalnom fazom u kombinaciji sa SELDI-TOF masenom spektrometrijom. Komparativna analiza hu manih serumskih glikoproteina izolovanih iz pulova normal nih seruma i PCa-seruma je ukazala na brojnost izolovanih molekula i na razliku u smislu njihovog pojedina~nog prisustva, mikroheterogenosti i relativne zastupljenosti. Generalno, uo~eno je pove}anje sijalinizacije i smanjenje fukozilacije kod PCa. Rezultati dobijeni u ovom radu ukazuju na to da pristup u kome se u inicijalnoj fazi ispitivanja koriste lektini uske specifi~nosti smanjuje kompleksnost uzorka, kao i na specifi~ne promene glikoproteinskog profila, koje mogu biti relevantne za pobolj{anje pra}enja progresije PCa.

show abstract

“…2), in the NANA residue, except for the reducing end, the 2-keto position is occupied by glycoside linkages. However, it is also known that the sialidase activity also changes in certain cancers, 12) and this will supply free sialic acids to the inflamed region. In addition, Eguchi et al reported the NANA-specific release from the cell surface sialyl Lewis X by ROS treatment.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Reaction between Sialic Acid (N-Acetylneuraminic Acid) and Hydrogen Peroxide

Iijima

Takahashi

Ikegami

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

N-Acetylneuraminic acid (NANA) is a sialic acid, which occurs in mammals, certain mollusks, and microorganisms, and is found in the oligosaccharide chains of glycoproteins and glycolipids.1) Moreover, NANA exists as a capping sugar in the oligosaccharide chains. These facts suggest the important role of NANA as a surface recognition molecule. Thus, NANA-related factors such as sialyl Lewis X, selectin, and siglec family proteins are being studied. 2,3) In our previous paper, we reported the scavenger effect of NANA in which NANA reacts with hydrogen peroxide (H 2 O 2 ) as an antidote; in this reaction, water, carbon dioxide, and the decarboxylated product of NANA-4-(acetylamino)-2,4-dideoxy-Dglycero-D-galacto-octonic acid (ADOA)-were generated. 4)Such a reaction was known to occur between H 2 O 2 and a-ketocarboxylic acids such as pyruvic acid, 5) but in the case of NANA, which is the most complex kind of a-ketocarboxylic acid, this was not reported prior to our study. It is thought that due to the low reactivity of NANA and H 2 O 2 in low-pH solution, this reaction was not detected until very recently. Although the reactivity of pyruvic acid and H 2 O 2 is also strikingly pH-dependent and the reaction occurs under conditions of low pH, it is sufficiently detectable.5) In contrast, in the case of NANA, it was thought that the reaction velocity was extremely small at low pH, such as that in the NANA solution itself, hence rendering it difficult to detect a significant decrease in the amount of H 2 O 2 . If this reaction is occurred in the living bodies, it seemed that reaction velocity became fast under the physiology state. In this paper, we report the rate constant (k) of the NANA-H 2 O 2 reaction under different pH and temperature conditions. MATERIALS AND METHODSMaterials N-Acetylneuraminic acid (Cat. No. 00622-31; Nacalai Tesque, Inc., Japan) was dissolved in water, and the pH of the solution was adjusted to 7.0-7.5 with sodium hydroxide; a(2→8) homodimer of N-acetylneuraminic acid (DP2) sodium salt (Cat. No. 00640-0431; Nacalai Tesque, Inc.) was dissolved in water. The pH value of this 10-mM solution was 6.6; The H 2 O 2 solution for atomic absorption spectrophotometry (Cat. No. 085-04056), which did not contain any stabilizer agents, was obtained from Wako Pure Chemical Industries Ltd., Japan.Analysis of the Reaction Product The analysis of the compound was based on conventional methods: Mass spectra were measured using fast atom bombardment mass spectrometry (FAB-MS) and high-resolution (HR)-FAB-MS.1 H-NMR spectra was recorded at 600 MHz ( 1 H-NMR) with a pulse Fourier transform NMR spectrometer by using tetramethylsilane (TMS) as an internal standard. Chemical shifts are reported in ppm downfield from TMS. solution in a 96-well microtiter plate was incubated with nine volumes of aqueous peroxide color reagent for 30 min at room temperature, and the optical density at 550 nm was measured.2. Since 30 min of incubation is required for color development when using the PeroxiDetect TM KIT, this method is not ...

show abstract

Sialidase and malignancy: A minireview

Cited by 138 publications

References 82 publications

Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin β4

Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin β4

Evaluation of the Pattern of Human Serum Glycoproteins in Prostate Cancer

Characterization of the Reaction between Sialic Acid (N-Acetylneuraminic Acid) and Hydrogen Peroxide

Contact Info

Product

Resources

About