Sialic Acid-Mimic Peptides As Hemagglutinin Inhibitors for Anti-Influenza Therapy

Matsubara, Takahiro; Onishi, Ai; Saito, Tomomi; Shimada, Aki; Inoue, Hiroki; Taki, Takao; Nagata, Kyosuke; Okahata, Yoshio; Sato, Toshinori

doi:10.1021/jm1002183

Cited by 100 publications

(141 citation statements)

References 39 publications

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“…Thereafter, a peptide was optimized for HA binding by selection from a phage‐displayed random peptide library 24. This work also showed a multivalent improvement of its binding properties by conjugation to either an amphiphilic N ‐stearoyl derivative, which formed self‐assembled multivalent structures with enhanced binding capabilities or by presenting up to six peptide copies on a carbosilane dendrimer 24, 30, 31…”

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confidence: 92%

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Multivalent Peptide–Nanoparticle Conjugates for Influenza‐Virus Inhibition

et al. 2017

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To inhibit binding of the influenza A virus to the host cell glycocalyx, we generate multivalent peptide–polymer nanoparticles binding with nanomolar affinity to the virus via its spike protein hemagglutinin. The chosen dendritic polyglycerol scaffolds are highly biocompatible and well suited for a multivalent presentation. We could demonstrate in vitro that by increasing the size of the polymer scaffold and adjusting the peptide density, viral infection is drastically reduced. Such a peptide–polymer conjugate qualified also in an in vivo infection scenario. With this study we introduce the first non‐carbohydrate‐based, covalently linked, multivalent virus inhibitor in the nano‐ to picomolar range by ensuring low peptide‐ligand density on a larger dendritic scaffold.

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confidence: 92%

“…Alternative ligands to SA are peptides targeting HA 24, 25, 26. Peptides may have several advantages, including higher affinity and selectivity.…”

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confidence: 99%

Multivalent Peptide–Nanoparticle Conjugates for Influenza‐Virus Inhibition

et al. 2017

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“…HA H1 and H3 subtypes were prepared from influenza A/New Caledonia/20/99 (H1N1) and A/New York/55/2004 (H3N2) viruses, respectively (29). The A/Puerto Rico/8/34 (H1N1) and A/Aichi/2/68 (H3N2) IFVs have been previously described (15). IFVs were grown in allantoic sacs of 11-d-old embryonated eggs and resuspended in 10 mM Tris·HCl (pH 7.8) containing 1 mM DTT and 20% (wt/vol) glycerol and stored at −80°C until use (30).…”

Section: Methodsmentioning

confidence: 99%

“…Seasonal H1 and H3 subtypes of influenza A virus were analyzed by EIS, using the peptide-terminated BDD electrode. The viral titer of IFV stock was determined by the plaque assay, using Mardin-Darby canine kidney epithelial cells (15). The IFV stock solution was thawed and diluted with PBS, and the electrode was incubated in H1N1 solution (1-400 pfu) for 45 min.…”

Section: Significancementioning

confidence: 99%

“…1). The sialic acidmimic pentapeptide Ala-Arg-Leu-Pro-Arg was identified by serial selection from random peptide libraries, using phage-display technology (15). In contrast to the standard sialyloligosaccharide receptor, this peptide was predicted to be able to capture HA and, hence, IFV (16,17): When IFV is incubated with a sialylated electrode, NA digestion of the electrode-bound sialyloligosaccharide can reduce the sensitivity of IFV detection, whereas the sialic acid-mimic peptide is free from risk for NA digestion.…”

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Highly sensitive detection of influenza virus by boron-doped diamond electrode terminated with sialic acid-mimic peptide

Matsubara

Ujie

Yamamoto

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The progression of influenza varies according to age and the presence of an underlying disease; appropriate treatment is therefore required to prevent severe disease. Anti-influenza therapy, such as with neuraminidase inhibitors, is effective, but diagnosis at an early phase of infection before viral propagation is critical. Here, we show that several dozen plaque-forming units (pfu) of influenza virus (IFV) can be detected using a boron-doped diamond (BDD) electrode terminated with a sialic acid-mimic peptide. The peptide was used instead of the sialyloligosaccharide receptor, which is the common receptor of influenza A and B viruses required during the early phase of infection, to capture IFV particles. The peptide, which was previously identified by phage-display technology, was immobilized by click chemistry on the BDD electrode, which has excellent electrochemical characteristics such as low background current and weak adsorption of biomolecules. Electrochemical impedance spectroscopy revealed that H1N1 and H3N2 IFVs were detectable in the range of 20-500 pfu by using the peptide-terminated BDD electrode. Our results demonstrate that the BDD device integrated with the receptor-mimic peptide has high sensitivity for detection of a low number of virus particles in the early phase of infection.influenza virus | boron-doped diamond electrode | sialic acid-mimic peptide | electrochemical impedance spectroscopy | click chemistry

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Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials

Liu

Zhang

et al. 2020

Advanced Science

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Waterborne viruses frequently cause disease outbreaks and existing strategies to remove such viral pathogens often involve harsh or energy‐consuming water treatment processes. Here, a simple, efficient, and environmentally friendly approach is reported to achieve highly selective disinfection of specific viruses with living engineered biofilm materials. As a proof‐of‐concept, Escherichia coli biofilm matrix protein CsgA was initially genetically fused with the influenza‐virus‐binding peptide (C5). The resultant engineered living biofilms could correspondingly capture virus particles directly from aqueous solutions, disinfecting samples to a level below the limit‐of‐detection for a qPCR‐based detection assay. By exploiting the surface‐adherence properties of biofilms, it is further shown that polypropylene filler materials colonized by the CsgA‐C5 biofilms can be utilized to disinfect river water samples with influenza titers as high as 1 × 10 7 PFU L −1 . Additionally, a suicide gene circuit is designed and applied in the engineered strain that strictly limits the growth of bacterial, therefore providing a viable route to reduce potential risks confronted with the use of genetically modified organisms. The study thus illustrates that engineered biofilms can be harvested for the disinfection of pathogens from environmental water samples in a controlled manner and highlights the unique biology‐only properties of living substances for material applications.

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Sialic Acid-Mimic Peptides As Hemagglutinin Inhibitors for Anti-Influenza Therapy

Cited by 100 publications

References 39 publications

Multivalent Peptide–Nanoparticle Conjugates for Influenza‐Virus Inhibition

Multivalent Peptide–Nanoparticle Conjugates for Influenza‐Virus Inhibition

Highly sensitive detection of influenza virus by boron-doped diamond electrode terminated with sialic acid-mimic peptide

Virus Disinfection from Environmental Water Sources Using Living Engineered Biofilm Materials

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