Sialic Acid Imprinted Polymer-Coated Quartz Crystal Microbalance

Kugimiya, Akimitsu; Yoneyama, Hidenobu; Takeuchi, Toshio

doi:10.1002/1521-4109(200011)12:16<1322::aid-elan1322>3.0.co;2-j

Cited by 41 publications

(16 citation statements)

References 15 publications

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“…IAA and indolebutyric acid (IBA) bearing a carboxyl group showed a strong interaction with the polymers, and other indole derivatives bearing no carboxyl groups, including indole, indole-3-ethanol (IEt), and indene, showed no interaction with the polymers in both chloroform/ acetic acid (99.9:0.1) 1 _ eluent and acetonitrile 2 _ eluent systems. 15 These results suggest that the carboxyl group should participate in binding to the amino groups of polymers.…”

Section: Nmr Titration Studiesmentioning

confidence: 95%

Effects of 2-Hydroxyethyl Methacrylate on Polymer Network and Interaction in Hydrophilic Molecularly Imprinted Polymers

Kugimiya

Takeuchi

1999

ANAL. SCI.

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Section: Nmr Titration Studiesmentioning

confidence: 95%

Effects of 2-Hydroxyethyl Methacrylate on Polymer Network and Interaction in Hydrophilic Molecularly Imprinted Polymers

Kugimiya

Takeuchi

1999

ANAL. SCI.

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“…[ 23 ] Conductometric measurements using molecularly imprinted polymers had shown a 5 × 10 − 9 M detection limit for atrazine. [ 24 ] On the other hand, fl uorescent sensing of dichloro diphenyl trichloroethane (DDT) using also molecularly imprinted polymers had a sensing limit of 50 ppt.…”

Section: Regeneration and Rebindingmentioning

confidence: 99%

SPR Detection of Dopamine Using Cathodically Electropolymerized, Molecularly Imprinted Poly‐p‐aminostyrene Thin Films

Dutta

Pernites

Danda

et al. 2011

Macro Chemistry & Physics

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A molecularly imprinted polymer (MIP) fi lm for dopamine (DP) sensing is fabricated from cathodically electrodeposited p -aminostyrene (PAS) on electrode surfaces in a surface plasmon resonance (SPR) spectroscopy setup. The monomer is more commonly used in monolithic MIP free-radical bulk polymerizations. The fi lm growth and rebinding of DP are monitored by electrochemical-SPR (EC-SPR) spectroscopy. UV-Vis, IR spectroscopy, XPS, AFM, and electrochemistry methods are used to characterize the fi lm. High selectivity against analogous analytes and up to picomolar detection of DP is demonstrated. The reusability of the sensor is also established. Theoretical modeling studies with AM1 calculations predict H-bonding in a stable prepolymerization complex in solution prior to MIP fi lm formation. intermolecular forces of association. Covalent [ 8 ] and noncovalent interactions [ 9 ] are the primary classifi cation attributed to the association mechanism in MIP. In the case of non-covalent binding, the interactions between monomer or polymer and template molecule include Van der Waals forces, dipole-dipole, and hydrogen (H)-bonding. The choice of crosslinking monomers with proper functionality plays an important role in MIP. Much effort has been directed toward the optimal choice of monomer and crosslinker combinations for the effective imprinting of the template molecule, enabling both covalent and non-covalent associations. [ 10 ] While the monolith approach is commonly utilized in making MIPs for separations or chromatography applications, the preparation of thin MIP fi lms is essential for effective and direct coupling of the sensing element fi lm onto the transducer surface in a sensor device. The bulk MIP approach such as in the case of monolith usually faces several shortcomings such as slow binding kinetics, poor selectivity, low capacity, or less template loading, and require substantial amounts of template for imprinting. In situ monitoring of the release and rebinding of the template molecule in bulk MIP is relatively more diffi cult since the imprinted

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“…Kugimiya et al. prepared sialic acid‐imprinted polymers for quartz crystal microbalance sensing, Sellergren et al. demonstrated bioimaging for cell surface glycans using sialic acid‐MIP particles .…”

Section: Introductionmentioning

confidence: 99%

Post‐Imprinting‐Modified Molecularly Imprinted Nanocavities with Two Synergetic, Orthogonal, Glycoprotein‐Binding Sites to Transduce Binding Events into Fluorescence Changes

et al. 2019

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We developed sensitive and selective polymeric nanocavities for glycoprotein detection using a novel molecular imprinting technique involving post‐imprinting modification (PIM). It allowed the introduction of two different interaction sites and a fluorescent reporter within each nanocavity. The hepatocellular carcinoma biomarker α‐fetoprotein (AFP) was used as a model glycoprotein. For PIM‐based molecular imprinting, AFP conjugated with polymerizable groups by disulfide bonding was prepared and immobilized on a 4‐carboxy‐3‐fluorophenylboronic acid‐immobilized substrate by cyclic diester formation between AFP glycans in an oriented immobilization manner, facilitating the creation of homogeneous AFP‐imprinted nanocavities. Surface‐initiated controlled/living radical polymerization was performed with a functional monomer, co‐monomer, and crosslinker. The disulfide bonds and cyclic diesters were cleaved to create AFP‐imprinted nanocavities, generating free thiol groups present inside the nanocavities only. A thiol‐reactive fluorescent dye was added to the nanocavities by in‐cavity PIM, yielding signaling AFP‐imprinted nanocavities capable of transducing AFP‐binding events into fluorescence changes. Reference proteins showed little response, while the limit of detection of AFP was 0.27 ng/mL (ca. 3.9 pM) in diluted human serum. Thus, the proposed in‐cavity PIM‐based molecular imprinting technique is effective for ELISA‐relevant, antibody‐free sensing systems for glycoproteins.

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Sialic Acid Imprinted Polymer-Coated Quartz Crystal Microbalance

Cited by 41 publications

References 15 publications

Effects of 2-Hydroxyethyl Methacrylate on Polymer Network and Interaction in Hydrophilic Molecularly Imprinted Polymers

Effects of 2-Hydroxyethyl Methacrylate on Polymer Network and Interaction in Hydrophilic Molecularly Imprinted Polymers

SPR Detection of Dopamine Using Cathodically Electropolymerized, Molecularly Imprinted Poly‐p‐aminostyrene Thin Films

Post‐Imprinting‐Modified Molecularly Imprinted Nanocavities with Two Synergetic, Orthogonal, Glycoprotein‐Binding Sites to Transduce Binding Events into Fluorescence Changes

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