Sialic acid, glutathione metabolism, and electrical conductivity in bovine mastitic udder tissue

Atroshi, Faik; Parantainen, J.; Kangasniemi, Reima; Sankari, Satu

doi:10.1111/j.1439-0396.1987.tb00164.x

Cited by 6 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, our study shows together with our earlier reports (Ali‐Vehmas et al., 1997; Malbe et al., 2003) that selenium may contribute to the protection of mammary gland from invading bacteria. Free radical generation and oxidative injury are thought to be important mechanisms contributing to morbidity, including mastitis (Atroshi et al., 1987, 1989, 2000). However, the mechanism by which the release of O 2 results in bactericidal effects is poorly understood, but is generally held to be related to the further generation of more reactive radical species, such as OH • , singlet oxygen and hypochlorite or hypothiocyanate (Del Maestro, 1980).…”

Section: Discussionmentioning

confidence: 99%

“…Studies in dairy cows have shown that selenium deficiency is associated with nutritional muscular dystrophy, cystic ovaries, retained placental membranes, metritis and infertility (Jukola et al., 1996; Hemingway, 2003). Other studies have shown evidence that selenium deficiency is associated with increased prevalence to intramammary infections (Smith et al., 1984, 1997; Atroshi et al., 1987, 1989; Erskine et al., 1989, 1990; Malbe et al., 1995; Ryan, 1995).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Possible involvement of selenium inStaphylococcus aureusinhibition in cow's whey

Malbe

Attila

Atroshi

2005

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

Previous investigations have shown that selenium supplementation inhibits growth of mastitis pathogens in cow's milk. The present study was performed to clarify the role of selenium in defence mechanisms of mammary gland. We have examined the effects of selenium supplementation on Staphylococcus aureus growth inhibiting activity in whey. Six selenium-supplemented and six non-supplemented Estonian dairy cows were used for this study. Selenium-supplemented cows received 4 mg organic selenium in the form of selenium yeast in their daily diet for 8-week period. Cows from non-supplemented group received the same amount of yeast free of selenium in their diet. All cows had initially low blood glutathione peroxidase (GPx) activity (

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Possible involvement of selenium inStaphylococcus aureusinhibition in cow's whey

Malbe

Attila

Atroshi

2005

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…Much less information is available from studies carried out using dietary antioxidants and mycotoxins (Rizzo et al 1994;Abel & Gelderblom, 1998;Yin et al 1998;Belmadani et al 1999;Galvano et al 2001;Gautier et al 2001). The protective properties of antioxidants probably result from their ability to act as superoxide anion scavengers, thereby protecting cell membranes from mycotoxin-induced damage; in some cases, antioxidant vitamins may play a role in preventing mycotoxicosis (Atroshi et al 1987).…”

mentioning

confidence: 99%

Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)

et al. 2005

View full text Add to dashboard Cite

Recent findings have suggested that oxidative damage might contribute to the cytotoxicity and carcinogenicity of aflatoxin B1 (AFB1). Induction of oxidative stress also plays an important role in the toxicity of another mycotoxin, ochratoxin A (OTA). In the present study, the protective effect of cyanidin-3-O-b-glucopyranoside (C-3-G; an anthocyanin contained in oranges, blackberries, strawberries and cranberries) against AFB1-and OTA-induced cytotoxicity was investigated in a human hepatoma-derived cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2). The ability of C-3-G to reduce the production of reactive oxygen species (ROS), the inhibition of protein and DNA synthesis and the apoptosis caused by the two mycotoxins was also investigated in both cell lines. Our experiments proved the significant cytoprotective effect of C-3-G in vitro against OTA-and AFB1-induced cell damage. In particular, 24 h of pretreatment with 50 mM-C-3-G inhibited the cytotoxicity of 10 mM-AFB1 (by 35 %) and of 10 mM-OTA (by 25 %) in Hep G2 cells (P,0·001) and of 10 mM-AFB1 (by 10 %, P,0·01) and of 10 mM-OTA (by 14 %, P,0·05) in CaCo-2 cells. Moreover, 50 mM-C-3-G attenuated ROS production induced by the two toxins in both cell lines (P,0·05). Inhibition of DNA and protein synthesis induced by the mycotoxins was counteracted by pretreatment with the antioxidant at 50 mM. Similarly, apoptotic cell death was prevented as demonstrated by a reduction of DNA fragmentation and inhibition of caspase-3 activation. The in vitro free-radical scavenging capacity of the anthocyanin was tested with the Briggs-Rauscher oscillating reaction. This system works at pH approximately 2. The results showed good scavenging power, in accordance with the observed inhibition of ROS production.

show abstract

“…Therefore we suggested that the increase of this enzyme activity in the inflammed tissue would represent a defence against the oxygen free radicals formed during peroxidation of arachidonic acid-like lipids, and be at the same time responsible for the markedly increased formation of PGEs in inflammation (ATROSHI et al, 1987b). Yet another GSH-enzyme, cytochrome P-450 was also increased in the mastitic udder tissue (ATROSHI et al, 1987~).…”

Section: Discussionmentioning

confidence: 90%

“…':-:' P < 0.01. The original values for the various biochemical traits can be found elsewhere(ATROSHI et al, 1987).…”

mentioning

confidence: 99%

Role of Plasma Fatty Acids, Prostaglandins and Antioxidant Balance in Bovine Mastitis

Atroshi

Rizzo

Kangasniemi

et al. 1989

Journal of Veterinary Medicine Series A

Self Cite

View full text Add to dashboard Cite

Summary Plasma levels of free fatty acids (FFA) were measured in cows with mastitis. FFA levels are known to be elevated in milk of affected cows, and similar increases were now observed in blood plasma. Of the long‐chain polyunsaturated fatty acids which serve as precursors for prostaglandins (PGs), leukotrienes (LT) and related eicosanoids, arachidonic acid (C20: 4 ω−6) was elevated by 156 per cent and C20: 3 (consisting of both ω, −3 and ω −6) 153 per cent; eicosapentaenoic acid (20: 5 ω −3), not present in the plasma of healthy animals, was detected in blood of mastitic animals. Arachidonic acid correlated positively with the plasma levels of PGs. Total plasma phospholipids were increased as well, and they were positively correlated with the levels of linoleic and arachidonic acids. Changes in FFA levels correlated positively with parameters of inflammation, like bovine serum albumin and serum N‐acetyl‐β‐D‐glucosaminidase, but negatively with the antioxidant status of the animals (vitamin E, glutathione peroxidase). As PGs and LTs are mediators of inflammation, and products of lipid peroxidation, the increase of plasma FFA could be related to tissue damage caused by oxygen free radicals. Similar processes are, however, involved also in host defence, e.g. in oxidative killing of pathogens. Zusammenfassung Zur Rolle der Plasma‐Fettsäuren und ‐Prostaglandine sowie des AntioxydansGleichgewichts bei der bovinen Mastitis Die Plasma‐Konzentrationen der freien Fettsäuren (FFA) wurden bei Kühen mit Mastitis gemessen. Es ist bekannt, daß die FFA‐Spiegel in der Milch solcher Tiere erhöht sind. ähnliche Konzentrationszunahmen ergaben sich nunmehr im Plasma. Von den langkettigen vielfach ungesättigten Fettsäuren, die als Vorstufen für die Bildung von Prostaglandinen (PG), Leukotrienen (LT) und von verwandten Eicosanoiden dienen, war die Arachidonsäure (C20:4 ω−6) um 156% und die C20: 3 (aus ω‐3 und ω−6 zusammengesetzt) um 153% erhöht; die Eicosapentaensäure (20: 5 ω‐3), die im Plasma gesunder Tiere nicht vorhanden war, wurde im Plasma von Tieren mit Mastitis gefunden. Der Plasma‐Arachidonsäure‐Spiegel war mit dem Plasma‐PG‐Spiegel positiv korreliert. Die Gesamt‐Plasma‐Phospholipide waren ebenfalls erhöht. Sie waren mit dem Plasma‐Linolsäure‐und Arachidonsäure‐Spiegeln positiv korreliert. Die Veränderung der FFA‐Spiegel waren mit Entzündungs‐Parametern wie dem Serumalbumin und der Serum‐N‐Azetyl‐β‐D‐Glucosaminidase positiv korreliert; mit dem Antioxydans‐Status (Vitamin E; Glutathion‐Peroxidase) waren sie negativ korreliert. Da PG und LT Entzündungs‐Mediatoren und Produkte der Lipid‐Peroxidation darstellen, könnte die Zunahme der FFA des Plasmas mit der durch Sauerstoffradikale bedingten Gewebsschädigung im Zusammenhang stehen. ähnliche Vorgänge spielen jedoch auch bei der Wirts‐Abwehr, wie z. B. bei der oxydativen Abtötung von pathogenen Keimen, eine Rolle.

show abstract

Sialic acid, glutathione metabolism, and electrical conductivity in bovine mastitic udder tissue

Cited by 6 publications

References 30 publications

Possible involvement of selenium inStaphylococcus aureusinhibition in cow's whey

Possible involvement of selenium inStaphylococcus aureusinhibition in cow's whey

Cyanidin-3-O-β-glucopyranoside, a natural free-radical scavenger against aflatoxin B1- and ochratoxin A-induced cell damage in a human hepatoma cell line (Hep G2) and a human colonic adenocarcinoma cell line (CaCo-2)

Role of Plasma Fatty Acids, Prostaglandins and Antioxidant Balance in Bovine Mastitis

Contact Info

Product

Resources

About