Si/SiO2 quantum dots cause cytotoxicity in lung cells through redox homeostasis imbalance

Stan, Miruna Silvia; Memet, Indira; Sima, Cornelia; Popescu, Traian; Teodorescu, V. S.; Dinischiotu, Anca

doi:10.1016/j.cbi.2014.06.020

Cited by 42 publications

(40 citation statements)

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“…This is in agreement with the morphology of QD aggregates in water, which was revealed by transmission electron microscopy images in a previous study [21]. Compared to water, the incubation in minimum essential medium (MEM) increased the hydrodynamic size of Si/SiO 2 QDs, which was even higher in the presence of 10% fetal bovine serum (FBS).…”

Section: Si/sio 2 Qds Propertiessupporting

confidence: 91%

“…By also mediating cell shape and stiffness, the modifications of actin cytoskeleton organization might be a cause of the altered morphology of lung fibroblasts as depicted previously by phase contrast microscopy [21]. The cytoskeletal arrangements and membrane ruffling after exposure to Si/SiO 2 QDs can be explained throughout the S-glutathionylation of actin induced by the same concentrations of QDs [21] because this post-translational process regulates cytoskeletal assembly [45]. The changes in actin cytoskeleton to enable the formation of membrane ruffles involved in engulfing the submicron particles were characteristic of the high concentration of QDs tested in the present study, suggesting the macropinocytosis as a particular type of QDs endocytosis [46].…”

mentioning

confidence: 68%

“…Actin filaments are components of a highly dynamic and complex system organized as a network known as the actin cytoskeleton, which contributes to multiple essential cellular functions, such as cell adhesion, motility, division, cytokinesis and cell signaling [44]. By also mediating cell shape and stiffness, the modifications of actin cytoskeleton organization might be a cause of the altered morphology of lung fibroblasts as depicted previously by phase contrast microscopy [21]. The cytoskeletal arrangements and membrane ruffling after exposure to Si/SiO 2 QDs can be explained throughout the S-glutathionylation of actin induced by the same concentrations of QDs [21] because this post-translational process regulates cytoskeletal assembly [45].…”

mentioning

confidence: 94%

“…and oxidative stress [21], which could trigger the induction of autophagy as a cellular survival mechanism. The interaction of autophagosomes with endosomes/lysosomes can be an important step in this process because these cellular structures are intracellular sites of QD localization [10].…”

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confidence: 99%

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Silicon‐based quantum dots induce inflammation in human lung cells and disrupt extracellular matrix homeostasis

et al. 2015

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Quantum dots (QDs) are nanocrystalline semiconductor materials that have been tested for biological applications such as cancer therapy, cellular imaging and drug delivery, despite the serious lack of information of their effects on mammalian cells. The present study aimed to evaluate the potential of Si/SiO 2 QDs to induce an inflammatory response in MRC-5 human lung fibroblasts. Cells were exposed to different concentrations of Si/SiO 2 QDs (25-200 lgÁmL À1 ) for 24, 48, 72 and 96 h. The results obtained showed that uptake of QDs was dependent on biocorona formation and the stability of nanoparticles in various biological media (minimum essential medium without or with 10% fetal bovine serum). The cell membrane damage indicated by the increase in lactate dehydrogenase release after exposure to QDs was dose-and time-dependent. The level of lysosomes increased proportionally with the concentration of QDs, whereas an accumulation of autophagosomes was also observed. Cellular morphology was affected, as shown by the disruption of actin filaments. The enhanced release of nitric oxide and the increase in interleukin-6 and interleukin-8 protein expression suggested that nanoparticles triggered an inflammatory response in MRC-5 cells. QDs decreased the protein expression and enzymatic activity of matrix metalloproteinase (MMP)-2 and MMP-9 and also MMP-1 caseinase activity, whereas the protein levels of MMP-1 and tissue inhibitor of metalloproteinase-1 increased. The present study reveals for the first time that silicon-based QDs are able to generate inflammation in lung cells and cause an imbalance in extracellular matrix turnover through a differential regulation of MMPs and tissue inhibitor of metalloproteinase-1 protein expression.

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Section: Si/sio 2 Qds Propertiessupporting

confidence: 91%

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confidence: 68%

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confidence: 94%

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confidence: 99%

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Silicon‐based quantum dots induce inflammation in human lung cells and disrupt extracellular matrix homeostasis

et al. 2015

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“…Thus, Si/SiO2 QDs have been reported to cause cytotoxicity in lung cells [108]. In this study, Stan et al demonstrated for the first time the negative influence of silicon QDs on cellular redox homeostasis and glutathione distribution in human MRC-5 lung fibroblastic cell line.…”

Section: Unresolved Problems Challenges and Controversiesmentioning

confidence: 63%

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Volkov

2015

Biochemical and Biophysical Research Communications

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a b s t r a c tThe review addresses the current state of progress in the use of ultra-small nanoparticles from the category of quantum dots (QDs), which presently embraces a widening range of nanomaterials of different nature, including "classical" semiconductor groups III-V and II-VI nanocrystals, along with more recently emerged carbon, silicon, gold and other types of nanoparticles falling into this class of nanomaterials due to their similar physical characteristics such as small size and associated quantum confinement effects. A diverse range of QDs applications in nanomedicine has been extensively summarised previously in numerous publications. Therefore, this review is not intended to provide an all-embracing survey of the well documented QDs uses, but is rather focused on the most recent emerging developments, concepts and outstanding unresolved problematic and sometimes controversial issues. Over 125 publications are overviewed and discussed here in the context of major nanomedicine domains, i.e. medical imaging, diagnostics, therapeutic applications and combination of them in multifunctional theranostic systems.© 2015 Elsevier Inc. All rights reserved. Quantum dots in nanomedicine: recent trendsWithin a steadily increasing database of diverse nanomaterials reported as suitable for applications in nanomedicine [1e10], quantum dots (QDs) deservedly occupy a special niche as nanoparticles with a unique track record full of high expectations, dramatic pitfalls and often controversial experimental evidence. From the early optimistic aspirations of them as breakthrough tools for multipurpose in vitro and in vivo applications [11e17], they have been subject to a period of partial relinquishment following the sombre realisation that in the original unmodified state QDs did not stand a chance to deserve a unanimous approval as imaging or drug delivery vehicles in humans, due to their intrinsic toxicity and lack of biodegradability perspective. The disappointingly low yield of exploitable outputs following the initial rounds of heavy investments in the field has also contributed to the overall decline in research productivity metrics. The analysis of the recent ten years' trends in publications number in this area related to the QDs medical applications in vivo in general (Fig. 1 A), as well as for imaging and diagnostics purposes (Fig. 1 B, C) according to Thomson Reuters Web of Science™ database clearly reflects such "cooling down" period after 2010 resulting in a significant reduction in the initially steady exponential growth of publication rates. This trend has been partially or completely reversed by 2014 and it is intriguing whether we will see it sustained in 2015 and beyond.Such returning enthusiasm has been reinvigorated by a number of objective tendencies in the related technological developments, including the arrival of innovative nanotechnology-enabled tools for diagnostic and ex vivo imaging applications [18,19], the arrival of new types of QDs of alternative nature offering the opportunities of r...

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Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Huang

Tang

2020

J of Applied Toxicology

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Quantum dots (QDs), as one of the emerging nanomaterials, have been widely studied by scientists due to their advantages and potential in bioimaging, especially cell imaging. Cadmium (Cd)‐based QDs, which have the best photoluminescence property, have received widespread attention. However, due to the obvious toxicity problem of these QDs, their cell imaging application is hindered. Recently, the emergence of Cd‐free‐metal and metal‐free QDs with lower toxicity makes people consider that Cd‐based QDs may be substituted by these QDs. However, problems of these QDs in cytotoxicity also cannot be ignored. Some reports claimed that their prepared emerging QDs for cell imaging were low toxicity, but there still exist up‐regulation of oxidative stress, inflammation, and apoptosis from other reports. And, up to now, few reports have separately summarized and discussed the issues of cell imaging and cytotoxicity of different types of QDs. Therefore, in this review, we classify QDs as following three types, Cd‐based, Cd‐free‐metal and metal‐free QDs, focus on the cell, the essential unit of life, discuss cell imaging application and cytotoxicity of QDs, and finally elucidate main mechanisms of cytotoxicity respectively. This review provides reference for the toxicity evaluation of QDs and highlights the potential cytotoxicity of Cd‐free QDs.

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Si/SiO2 quantum dots cause cytotoxicity in lung cells through redox homeostasis imbalance

Cited by 42 publications

References 72 publications

Silicon‐based quantum dots induce inflammation in human lung cells and disrupt extracellular matrix homeostasis

Silicon‐based quantum dots induce inflammation in human lung cells and disrupt extracellular matrix homeostasis

Quantum dots in nanomedicine: recent trends, advances and unresolved issues

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

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