Shuyu capsules relieve liver‑qi depression by regulating ERK‑CREB‑BDNF signal pathway in central nervous system of rat

Abstract

The purpose of this study was to investigate the possible therapeutic mechanism of Shuyu capsules in liver-qi depression. Liver-qi depression rats were prepared based on chronic unpredictable mild stress (CUMS) and delayed constraint. Rats were gavaged with Shuyu capsule, fluoxetine, Radix Bupleuri and Radix Paeoniae Alba to constrct rat models. Body weight test, sucrose preference test and open-field test were applied to test rat models. Western blot analysis and quantitative real-time PCR was applied to determine the relative expression of extracellular signal-regulated protein kinase (ERK), cyclic AMP response element binding protein (CREB) and brain-derived neurotrophic factor (BDNF) in hippocampus and frontal lobe tissues. ELISA was used to detect the content of BDNF in serum. Body weight, sugar intake and total distance were significantly decreased in depression group compared with control. The four drugs significantly increased levels of these factors. Compared with control group, ERK, CREB and BDNF expression were significantly decreased in depression group in both hippocampus and frontal lobe tissues at both mRNA and protein level. Shuyu capsule and fluoxetine group showed a significant increase in the expression of ERK, CREB and BDNF at mRNA, p-ERK and p-BDNF at protein level. Compared with Radix Paeoniae Alba, Radix Bupleuri were better in the rescue of ERK, CREB and BDNF expression. In conclusion, the pathogenesis of liver-qi depression associated with lower expression of ERK, CREB and BDNF in hippocampus and frontal. Shuyu capsule and main constitution alleviated the depressive-like behaviors and reversed the disruptions of the p-ERK, p-CREB and BDNF in stressed rats.

Baixiangdan Capsule and Shuyu Capsule Regulate Anger-out and Anger-in: GB1 Mediated GABA Can Regulate 5-ht Level in the Dorsal Raphe Nucleus

“…The results of this study also con rmed that these three evaluation methods can effectively evaluate the rat model of anger-out and anger-in, and BXD and SY can effectively correct abnormal anger tendency by changing the score of ABT, OFT and SPT. This is consistent with the results of relevant reports 64,65 .…”

Low‐level laser therapy 810‐nm up‐regulates macrophage secretion of neurotrophic factors via PKA‐CREB and promotes neuronal axon regeneration in vitro

“…Luo et al confirmed that activation of the cAMP/PKA‐CREB pathway can increase the secretion of BDNF in hippocampal neurons and ultimately promote the recovery of learning and memory impairment in rats under chronic unpredictable mild stress. Wang et al demonstrated that Shuyu capsules can increase the phosphorylation of CREB by activating the ERK‐CREB signal transduction pathway, thereby increasing the secretion of neurotrophic factors in the central nervous system of rats. At the same time, studies have revealed that LLLT can also promote the secretion of neurotrophic factors.…”

“…Many studies have shown that stimulation of the ERK‐cAMP response element‐binding (CREB) or protein kinase A (PKA)‐CREB pathway can promote the secretion of various neurotrophic factors. In contrast to the proinflammatory M1 macrophages, the M2 type is capable of secreting many types of neurotrophic factors . This finding implies that modulation of the polarization state of the macrophages in the SCI lesion can be used to promote nerve repair, which has become a focus of research into SCI recovery.…”

“…In contrast to the proinflammatory M1 macrophages, the M2 type is capable of secreting many types of neurotrophic factors. 10,11 This finding implies that modulation of the polarization state of the macrophages in the SCI lesion can be used to promote nerve repair, which has become a focus of research into SCI recovery. A number of approaches can be used to modify the polarization of macrophages and thereby increase the relative abundance of the M2 cell type, which would in turn increase the levels of secreted neurotrophic factors and effectively increase the growth of neuronal axons, ultimately promoting neuronal growth and repair.…”

Therapeutic of Candesartan and Music Therapy in Diabetic Retinopathy with Depression in Rats

“…BDNF could promote cell differentiation and proliferation of neural stem cells, has nutrition effects on many types of neurons, Evidence-Based Complementary and Alternative Medicine has a greater impact on the plasticity of neurons and the synthesis of neurotransmitters, and is also related to learning and memory [35]. In addition, other studies have shown that BDNF plays an important role in the pathogenesis of severe depression [34,36,37]. BDNF is involved in the survival of neurons and the regeneration of dopamine, 5-HT, and cholinergic neurons in the central nervous system, and it is also a molecular marker of synaptic plasticity [38].…”