Shrimp with knockdown of LvSOCS2, a negative feedback loop regulator of JAK/STAT pathway in Litopenaeus vannamei, exhibit enhanced resistance against WSSV

Wang, Sheng; Song, Xuan; Zhang, Zijian; Li, Haoyang; Lǚ, Kai; Yin, Bin; He, Jianguo; Li, Chaozheng

doi:10.1016/j.dci.2016.07.021

Cited by 39 publications

(13 citation statements)

References 44 publications

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“…This gene is a regulator that exerts a negative feedback on STAT and Wang et al . 58 demonstrated that shrimp knockdown for SOCS2 exhibit increased WSSV resistance. In fact, the inhibition of this pathway may have a direct effect on the production of AMPs such as Penaeidins , as demonstrated by Sun et al .…”

Section: Discussionmentioning

confidence: 99%

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Peruzza

Shekhar

Kumar

et al. 2019

Sci Rep

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Shrimp aquaculture is severely affected by WSSV. Despite an increasing effort to understand host/virus interaction by characterizing changes in gene expression (GE) following WSSV infection, the majority of published studies have focussed on a single time-point, providing limited insight on the development of host-pathogen interaction over the infection cycle. Using RNA-seq, we contrasted GE in gills of Litopenaeus vannamei at 1.5, 18 and 56 hours-post-infection (hpi), between WSSV-challenged and control shrimps. Time course analysis revealed 5097 differentially expressed genes: 63 DEGs were viral genes and their expression in WSSV group either peaked at 18 hpi (and decreased at 56 hpi) or increased linearly up to 56 hpi, suggesting a different role played by these genes during the course of infection. The remaining DEGs showed that WSSV altered the expression of metabolic, immune, apoptotic and cytoskeletal genes and was able to inhibit NF-κB and JAK/STAT pathways. Interestingly, GE changes were not consistent through the course of infection but were dynamic with time, suggesting the complexity of host-pathogen interaction. These data offer novel insights into the cellular functions that are affected during the course of infection and ultimately provide a valuable resource towards our understanding of the host-pathogen dynamics and its variation with time.

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Section: Discussionmentioning

confidence: 99%

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Peruzza

Shekhar

Kumar

et al. 2019

Sci Rep

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“…At present, the mammalian SOCS family of proteins is believed to consist of eight members: SOCS1-7 and the alternatively named cytokine-inducible Src homology 2 (SH2)-containing protein (CIS) [ 20 , 21 ]. The SOCS/CIS family is a group of intracellular proteins, several of which downregulate cytokines signaling following cytokines engagement of their specific receptors complex through a negative feedback loop [ 22 ].…”

Section: The Classification Structure and Action Mechanism Of Socs mentioning

confidence: 99%

SOCS molecules: the growing players in macrophage polarization and function

et al. 2017

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The concept of macrophage polarization is defined in terms of macrophage phenotypic heterogeneity and functional diversity. Cytokines signals are thought to be required for the polarization of macrophage populations toward different phenotypes at different stages in development, homeostasis and disease. The suppressors of cytokine signaling family of proteins contribute to the magnitude and duration of cytokines signaling, which ultimately control the subtle adjustment of the balance between divergent macrophage phenotypes. This review highlights the specific roles and mechanisms of various cytokines family and their negative regulators link to the macrophage polarization programs. Eventually, breakthrough in the identification of these molecules will provide the novel therapeutic approaches for a host of diseases by targeting macrophage phenotypic shift.

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“…Lv JAK silencing caused higher mortality 49 and knockdown of Lv SOC2, a negative feedback loop regulator of JAK/STAT pathway in L . vannamei , resulted in less WSSV infection 50 . More recently, Wang, W. et al ., 2019 reported that inhibition of the JAK/STAT pathway in Drosophila by WSV181 could enhance WSSV replication 51 .…”

Section: Discussionmentioning

confidence: 97%

Role of Clathrin Assembly Protein-2 Beta Subunit during White Spot Syndrome Virus Infection in Black Tiger Shrimp Penaeus monodon

Jatuyosporn

Laohawutthichai

Supungul

et al. 2019

Sci Rep

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White spot syndrome virus (WSSV) is one of the most lethal viruses severely affecting shrimp industry. This disease can cause 100% mortality of farmed shrimp within a week. This work aims to characterize clathrin assembly proteins in Penaeus monodon and investigate their roles in WSSV entry. In general, clathrin assembly proteins form complexes with specific receptors and clathrins, leading to clathrin-mediated endocytosis. Adaptor protein 2 (AP-2), which is responsible for endocytosis at plasma membrane, consists of four subunits including α, β2, μ2 and σ2. Knockdown of clathrin coat AP17, or σ subunit of AP-2 dramatically reduced WSSV infectivity. Similar results were observed, when shrimp were pre-treated with chlorpromazine (CPZ), an inhibitor of clathrin-dependent endocytosis. The complete open reading frames of AP-2β and μ subunits of P. monodon are reported. PmAP-2 β was up-regulated about 4-fold at 6 and 36 h post-WSSV infection. Knockdown of PmAP-2β delayed shrimp mortality during WSSV infection, of which WSSV intermediate early 1 gene expression was also down-regulated. Immunogold-labelling and transmission electron microscopy revealed that PmAP-2β co-localized with WSSV particles at plasma membrane. In addition, PmAP-2β-silencing significantly affected the expression levels of PmSTAT, PmDOME, PmDorsal and ALFPm3 during WSSV infection. It is possible that PmAP-2β is associated with the JAK/STAT and the Toll pathway.

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Shrimp with knockdown of LvSOCS2, a negative feedback loop regulator of JAK/STAT pathway in Litopenaeus vannamei, exhibit enhanced resistance against WSSV

Cited by 39 publications

References 44 publications

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

Temporal changes in transcriptome profile provide insights of White Spot Syndrome Virus infection in Litopenaeus vannamei

SOCS molecules: the growing players in macrophage polarization and function

Role of Clathrin Assembly Protein-2 Beta Subunit during White Spot Syndrome Virus Infection in Black Tiger Shrimp Penaeus monodon

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