Shrimp (Penaeus monodon) anti-lipopolysaccharide factor reduces the lethality of Pseudomonas aeruginosa sepsis in mice

Antimicrobial peptides (AMP) with antiviral activity against fish nodavirus

“…The amino acid sequence of cSALF is ECKFTVKPYLKRFQ-VYYKGRMWCP [29]. It exhibits antimicrobial ability against both Gram-positive and -negative bacteria and is able to form an amphipathic hairpin loop in the region of 20e21 amino acids in helical length, which can form an LPS-binding motif, or disrupt viral functions at the phospholipid membrane bilayer [29].…”

“…It exhibits antimicrobial ability against both Gram-positive and -negative bacteria and is able to form an amphipathic hairpin loop in the region of 20e21 amino acids in helical length, which can form an LPS-binding motif, or disrupt viral functions at the phospholipid membrane bilayer [29]. However, NNV is non-enveloped, and the anti-NNV mechanism of cSALF was achieved by agglutinating NNV capsid protein, similar to that of TH 1-5, and the agglutination was irreversible, indicating that the two peptides might change NNV virion or capsid protein from hydrophilic to hydrophobic, causing the formation of aggregates.…”

“…Three AMPs, including grouper epinecidin-1 (CP643-1) (Accession number AY294407) [28], tilapia hepcidin 1-5 (TH 1-5) [4], and cyclic shrimp anti-lipopolysaccharide factor (cSALF) [29], were gifts from Dr. J.Y. Chen.…”

Antimicrobial Peptides and their Potential Application in Inflammation and Sepsis

“…Parallel to this, the peptides were able to reduce the bacteria-induced produc tion of TNF-α in the animals. However, already at a concentration of 2 μg/ml the peptides exhibited significant cytotoxicity in HeLa, MCF-7, and HT1080 cell lines, impeding their use as an anti-infective drug [31].…”

Molecular characterization and phylogenetic analysis of an anti-lipopolysaccharide factor from the crucifix crab, Charybdis feriatus

“…Functional characterization of ALFs has been conducted by chemically synthesizing the LPS domain region, [16][17][18][19][20][21][22] and by recombinant expression of mature peptides. 9,13,[23][24][25][26][27][28] Biological activity reported so far for ALFs include antibacterial, 6,9,20 antifungal, 6,27 antiviral, 29,30 immunomodulatory, 31 antitumor, 32 anti-inflammatory, and antiprotozoal activity. 32,33 As per the previous studies, the structure of ALF was elucidated using nuclear magnetic resonance (NMR) spectroscopy, with the finding that it was composed of three α-helices packed against a four-stranded β-sheet.…”