Shrimp miR-S8 Suppresses the Stemness of Human Melanoma Stem-like Cells by Targeting the Transcription Factor YB-1

Yang, Fan; Wei, Jun; Zhang, Song; Zhang, Xiaobo

doi:10.1158/0008-5472.can-17-1375

Cited by 35 publications

(42 citation statements)

References 42 publications

(39 reference statements)

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“…When shrimp miR‐S8 was cotransfected with YB‐1 3′‐UTR plasmid, the luciferase activity was significantly decreased. It indicated that miR‐S8 suppresses the stemness of human melanoma stem‐like cells by targeting the transcription factor YB‐1 (F. Yang, Wei, Zhang, & Zhang, ). By regulating the expression of foxl3 by binding to foxl3 3′‐UTR wild‐type plasmid, mal‐miR‐9 was involved in promoting oocyte degeneration in the ovotestis, and stimulating spermatogenesis in spermatogonia in Monopterus albus (Y. Gao, Jia, Hu, & Li, ).…”

Section: Discussionmentioning

confidence: 99%

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

Zhou

Jia

Wan

et al. 2018

Molecular Reproduction Devel

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Mud crab Scylla paramamosain is a commercially important species widely cultured in China. It is well known that the eyestalk regulates reproductive activities in crustaceans. In our previous research, we found that the miR‐34 expression level in male eyestalk was significantly higher than that in females. Thus, we assumed that it may play an important role in regulating reproduction. In this study, we used bioinformatic tools to identify the target genes of miR‐34 in eyestalk. Six reproduction‐related genes with an intact 3′‐untranslated region (UTR), including molt‐inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), vitellogenesis‐inhibiting hormone, red pigment concentrating hormone, ecdysone receptor (EcR), and farnesoic acid methyltransferase (FAMeT) were identified. When the 3′‐UTR plasmid vectors of the six genes were cotransfected with miR‐34 mimics into 293FT cells, respectively, the luciferase activities of four genes (MIH, CHH, EcR, and FAMeT) were significantly decreased compared with that in the control group; on the contrary, when the six plasmid vectors were cotransfected with the miR‐34 inhibitor respectively, the luciferase activities of four genes (MIH, CHH, EcR, and FAMeT) were significantly higher than that in the control group. When agomiR‐34 and antagomiR‐34 were injected into the eyestalk respectively in vivo, the expression levels of the MIH, CHH, EcR, and FAMeT genes were detected by a quantitative real‐time polymerase chain reaction. The results showed that agomiR‐34 suppressed the expression of the four genes, whereas antagomiR‐34 enhanced their expression. These experimental results confirmed our hypothesis that miR‐34 may indirectly regulate reproduction via binding to the 3′‐UTRs of MIH, CHH, EcR, and FAMeT genes and suppressing their expression.

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Section: Discussionmentioning

confidence: 99%

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

Zhou

Jia

Wan

et al. 2018

Molecular Reproduction Devel

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“…Shrimp miRNA‐S8 inhibited the expression of the YB‐1 transcription factor in a cross‐species manner, targeting the 3′UTR of YB‐1 mRNA and mediating its degradation, leading to the suppression of tumorigenesis in melanoma cells (Yang et al . ). It has been suggested that antiviral miRNAs may have antitumour capacity because, in general terms, tumorigenesis results from a metabolic disorder of cells.…”

Section: Shrimp Micrornas With Antitumoral Activity: An Alternative Smentioning

confidence: 97%

The role of shrimp microRNAs in immune response and beyond

González-Duarte¹,

García‐Carreño²

2018

Reviews in Aquaculture

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MicroRNAs are a class of small non‐coding RNAs that regulate gene expression at post‐transcriptional level. Regulatory RNAs were discovered in the nematode Caenorhabditis elegans, since then, their biological functions have been studied on different animals, including marine organisms. Several species of penaeid shrimp are important in ecology and fishing, including aquaculture. Overcrowding in aquaculture calls for infectious diseases, which threaten the development of shrimp aquaculture worldwide. Given that microRNAs play crucial regulatory roles in a wide variety of biological processes, there is a scientific interest to understand their contribution on shrimp physiology and pathology, specifically during immune and stress response. Increasing evidence has shown that the expression of microRNAs is affected during virus or bacterial infection and upon stress in shrimp. This information provides valuable insights for a better understanding of shrimp biology by means of microRNA regulation to bacterial and viral diseases.

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“…Melanoma cell line MDA-MB-435 and breast cancer cell line MDA-MB-453 were purchased from American Type Culture Collection (Manassas, VA, USA). Melanoma stem cells and nonstem cells were sorted from MDA-MB-435 cells by our laboratory as previously described in Yang et al (24). Breast cancer stem cells and nonstem cells were sorted in the present study.…”

Section: Cell Culturesmentioning

confidence: 99%

“…Cancer stem cells and the corresponding cancer nonstem cells were sorted from 2 types of cancer cells (MDA-MB-453 and MDA-MB-435). MDA-MB-435 stem cells were obtained in our previous study (24). For the sorting of stem cells from MDA-MB-453 cells, the ALDH1-positive cells were sorted as cancer stem cells (Fig.…”

Section: Aldh1-positive Mda-mb-453 Breast Cancer Cells Have Stem Cellmentioning

confidence: 99%

“…22 and 23, the antiviral molecules of marine organisms induced by virus infection may play important roles in the antitumor pathways of humans. It is well known that a single miRNA has multiple target genes, suggesting that an miRNA could suppress the expression of genes from different species (24). At present, however, the impact of animal miRNAs on cancer stem cells has not been extensively explored.…”

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confidence: 99%

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Shrimp miRNA suppresses the stemness of human cancer stem cellsviathe PIN1 pathway

Zhang

2019

FASEB j.

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A tumor can be regarded as a cell mass with a biologic hierarchy that is orchestrated by cancer stem cells. The specific features of cancer stem cells may result from a series of gene expression regulatory mechanisms. As important regulatory factors of gene expression, microRNAs (miRNAs) have critical roles in the self‐renewal, pluripotency, differentiation, and tumorigenicity of cancer stem cells. However, the effects of animal miRNAs on cancer stem cells have not been investigated extensively. Here, we report that miRNA of a shrimp, Marsupenαeus jαponicus (mja‐miR‐35‐3p), which possesses antiviral activity in shrimp, could suppress the proliferation of melanoma and breast cancer stem cells, but not cancer nonstem cells, by arresting the cell cycle in the G1 phase and inducing apoptosis. Shrimp mja‐miR‐35‐3p (named mja‐miR‐35) targets the human peptidylprolyl cis/trans isomerase, never in mitosis gene a‐interacting 1 (PIN1) gene, which is up‐regulated in cancer stem cells. The results indicated that PIN1 silencing caused cell cycle arrest in the G1 phase, induced apoptosis, and decreased the sphere‐forming capacity of cancer stem cells, but not cancer nonstem cells, showing that PIN1 had beneficial effects against the stemness of cancer stem cells. The in vivo data revealed that shrimp mja‐miR‐35 suppressed the stemness of cancer stem cells in mice by inhibiting the PIN1 antiapoptotic–cell cycle pathway. These findings demonstrated that the miRNAs of aquatic animals might be an important source for discovering human antitumor drugs.—Zhang, S., Zhang, X. Shrimp miRNA suppresses the stemness of human cancer stem cells via the PIN1 pathway. FASEB J. 33, 10767–10779 (2019). http://www.fasebj.org

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Shrimp miR-S8 Suppresses the Stemness of Human Melanoma Stem-like Cells by Targeting the Transcription Factor YB-1

Cited by 35 publications

References 42 publications

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

The role of shrimp microRNAs in immune response and beyond

Shrimp miRNA suppresses the stemness of human cancer stem cellsviathe PIN1 pathway

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