Shrimp Immune System and Immune Responses

Rajendran, K. V.; Sreedharan, K.; Deepika, A.; Kulkarni, Amod

doi:10.1007/978-981-19-1268-9_2

Cited by 2 publications

(1 citation statement)

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“…Viral infection outbreak within the industry comes up because of the absence of adaptive and specific immune responses. The previous study shows that the transcription of IMNV mRNA can be reduced by RNA interference (RNAi) response, which recognizes the dsRNA fragment encoding IMNV (Rajendran et al, 2022). The development of dsRNA that comprise of the IMNV sequence as an antiviral agent against IMNV infection has emerged recently.…”

Section: Introductionmentioning

confidence: 99%

Media Selection and Fed-Batch Fermentation Strategy for dsRNA Plasmid Production inEscherichia coliGT115

Pancoro

Taufik

Wijayana

2023

Preprint

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Application of plasmid encoding synthetic dsRNA targeted IMNV genome (Infectious Myonecrosis Virus) can reduce viral replication in the shrimp industry by activating RNA interference (RNAi) response. Application of dsRNA plasmid as antiviral for IMNV in shrimp-farm need a huge quantity of plasmid. Bioreactor can be used for large-scale plasmid production to achieve high plasmid yield. Plasmid production in the bioreactor can be improved by selection of the host organism, the recombinant plasmid vector, the fermentation media, and the fermentation strategy. This research aim is to determine the fermentation media and fermentation strategy to produce recombinant dsRNA plasmid with high plasmid yield. Selection of fermentation media was conducted in a baffled flask with three different media. Then, the optimum media was used for optimization in bioreactor production with the addition of feeding media. As a result, plasmid production in TB media has a higher biomass growth rate and plasmid production rate than production in M9+Mod and LB+ media. Plasmid production in TB media in baffled-flask resulted in plasmid yield in 2.318 mg/L, 14-fold higher than M9+Mod (0.165 mg/L), and 34-fold higher than LB (0.068 mg/L). In bioreactor production, plasmid production in fed-batch fermentation in bioreactor resulted plasmid yield in 1.018 mg/L, 5-fold higher than batch fermentation (1.882 mg/L). Plasmid was confirmed in agarose gel electrophoresis at ~5750 bp and insert gene at 700 bp. The cultivation technique developed should be workable for the pilot scale. Downstream processing in plasmid production should be able to achieve plasmid with high concentration and purity.

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Section: Introductionmentioning

confidence: 99%

Media Selection and Fed-Batch Fermentation Strategy for dsRNA Plasmid Production inEscherichia coliGT115

Pancoro

Taufik

Wijayana

2023

Preprint

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Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Cox,

De Swaef,

Corteel

et al. 2024

Viruses

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White spot syndrome virus (WSSV) is marked as one of the most economically devastating pathogens in shrimp aquaculture worldwide. Infection of cultured shrimp can lead to mass mortality (up to 100%). Although progress has been made, our understanding of WSSV’s infection process and the virus–host–environment interaction is far from complete. This in turn hinders the development of effective mitigation strategies against WSSV. Infection models occupy a crucial first step in the research flow that tries to elucidate the infectious disease process to develop new antiviral treatments. Moreover, since the establishment of continuous shrimp cell lines is a work in progress, the development and use of standardized in vivo infection models that reflect the host–pathogen interaction in shrimp is a necessity. This review critically examines key aspects of in vivo WSSV infection model development that are often overlooked, such as standardization, (post)larval quality, inoculum type and choice of inoculation procedure, housing conditions, and shrimp welfare considerations. Furthermore, the usefulness of experimental infection models for different lines of WSSV research will be discussed with the aim to aid researchers when choosing a suitable model for their research needs.

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Shrimp Immune System and Immune Responses

Cited by 2 publications

References 165 publications

Media Selection and Fed-Batch Fermentation Strategy for dsRNA Plasmid Production inEscherichia coliGT115

Media Selection and Fed-Batch Fermentation Strategy for dsRNA Plasmid Production inEscherichia coliGT115

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

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