Shrimp hepatopancreatic parvovirus detection by combining loop-mediated isothermal amplification with a lateral flow dipstick

Loop-mediated Isothermal Amplification (LAMP) test for detection of Trypanosoma evansi strain B

“…The strategy is robust, specific and shows tolerance to several biological products that inhibit conventional PCR (Yamada et al, 2006;Kaneko et al, 2007), meaning that template DNA extraction may not be necessary. Besides, LAMP results can be visually inspected through colour change (Poon et al, 2006;Njiru et al, 2008b) or coupling with chromatographic LFD (Nimitphak et al, 2008) which significantly reduces the assay time.…”

“…Moreover these two methods showed identical detection limits with gel electrophoresis and real time monitoring (Table 3). Additionally, the applicability of SYBR Green I (Poon et al, 2006;Njiru et al, 2008b) and LFD in detection of LAMP product Nimitphak et al, 2008;Jeroenram et al, 2009) has been demonstrated. SYBR Green I bind to double-stranded DNA and the resulting DNAdye-complex gives a green colour (Fig 3a).…”

“…In LFD format, the FITC-labelled biotinylated LAMP product combines with gold-labelled anti-FITC in the sample well. The triple-labelled complex moves up and is captured by an immobilized biotin-binding protein (Test line) (Fig 3a) while the non-hybridized FITC probe binds to the gold-labelled anti-FITC to form a double complex without biotin and is trapped at the control line (Nimitphak et al, 2008).…”

Nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products

“…The same generic LFD strips has been applied on Taura syndrome virus [35], hepatopancreatic parvovirus [36], infectious myonecrosis virus [37], infectious hypodermal and hematopoietic necrosis virus [38], yellow head virus [39], Laem-Singh virus [40], Penaeus monodon nucleopolyhedrovirus or monodon baculovirus [41] and white spot syndrome virus [42] for shrimp viruses detection; as well as fish infectious spleen and kidney necrosis virus detection [43] and cyprinid herpes virus-3 or koi herpes virus [44]. In all cases, loop mediated isothermal amplification (LAMP) has been utilized for nucleic acid amplification, instead of traditional PCR, which is used in the proposed method.…”

Detection of Group 1 Trypanosoma brucei gambiense by Loop-Mediated Isothermal Amplification

“…The technique has been used to develop LAMP tests specific for the subgenus Trypanozoon (18,34,45) and Trypanosoma brucei rhodesiense (35), but so far, there is no LAMP test for T. b. gambiense. The major advantages of LAMP include (i) rapidity and the use of six to eight primers providing high specificity; (ii) ability of the technique to amplify target DNA from partially processed template; (iii) requirement for only a simple heating device, such as a water bath; and (iv) results that can be inspected visually through the use of varied detection formats, such as turbidity (28), fluorescent dye (5), probes (1,27), lateral-flow dipstick (LFD) format (31), and microfluidic chips (13). LAMP has attracted much interest as an easily applicable yet highly sensitive molecular tool with great potential for diagnosis in resource-poor rural settings, where HAT typically occurs.…”