Shrimp hepatopancreatic deoxyribonuclease — purification and characterization as well as comparison with bovine pancreatic deoxyribonuclease

Chou, Ming-Yi; Liao, Ta–Hsiu

doi:10.1016/0304-4165(90)90019-s

Cited by 17 publications

(35 citation statements)

References 36 publications

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“…The predicted crab protein shows 64% identity at the amino acid level with shrimp nuclease from Penaeus japonicus (Chou and Liao 1990;Wang et al 2000). Analysis of the crab sequence by SMART software (Schultz et al 2000) disclosed that the protein comprises a 27-aa signal peptide and a DNA/RNA nonspecific endonuclease domain (NUC domain).…”

Section: Cloning and Purification Of A Kamchatka Crab Nucleasementioning

confidence: 99%

A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas

Shagin¹,

Rebrikov²,

Kozhemyako³

et al. 2002

Genome Res.

229

198

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We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is essentially higher than that for nonperfectly matched duplexes of the same length. Thus, DSN differentiates between one-nucleotide variations in DNA. We developed a novel assay for single nucleotide polymorphism (SNP) detection based on this unique property, termed "duplex-specific nuclease preference" (DSNP). In this innovative assay, the DNA region containing the SNP site is amplified and the PCR product mixed with signal probes (FRET-labeled short sequence-specific oligonucleotides) and DSN. During incubation, only perfectly matched duplexes between the DNA template and signal probe are cleaved by DSN to generate sequence-specific fluorescence. The use of FRET-labeled signal probes coupled with the specificity of DSN presents a simple and efficient method for detecting SNPs. We have employed the DSNP assay for the typing of SNPs in methyltetrahydrofolate reductase, prothrombin and p53 genes on homozygous and heterozygous genomic DNA.

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Section: Cloning and Purification Of A Kamchatka Crab Nucleasementioning

confidence: 99%

A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas

Shagin¹,

Rebrikov²,

Kozhemyako³

et al. 2002

Genome Res.

229

198

View full text Add to dashboard Cite

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“…This adsorbent has been used successfully for the puri¢cation of endonucleases from S. cerevisiae [28,30], shrimp hepatopancreas [52], S. racemosum [34], Leishmania sp. [37] and B. taurus [77].…”

Section: Puri¢cationmentioning

confidence: 99%

“…During the initial puri¢cation steps, one of the primary aims is to get rid of the colored impurities contributed by pigments of the organelles and this is achieved either by extraction with acetone^water mixture (4:1 v/v) [42] or by a simple wash with ammonium chloride followed by high speed centrifugation [47,48]. In most cases apart from sonication [52], grinding with glass beads or sand [11,21,41,42] has also been used for disrupting the cells. In general, ammonium sulfate precipitation is also used as a preliminary puri¢cation step.…”

Section: Puri¢cationmentioning

confidence: 99%

“…In animals, they have been isolated from various organelles, for example, the hepatopancreas of shrimp [52], rat liver nuclei [50], Bos taurus mitochondria [61] and embryos of Drosophila melanogaster [54]. …”

Section: Occurrence and Localizationmentioning

confidence: 99%

“…[37] and B. taurus [77]. Endonucleases from S. racemosum [34], C. echinulata [38], rye germ ribosomes [48] and shrimp hepatopancreas [52] have been puri¢ed on hydrophobic matrices like phenyl-and octyl-Sepharose.…”

Section: Puri¢cationmentioning

confidence: 99%

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Sugar non-specific endonucleases

Rangarajan¹

2001

FEMS Microbiology Reviews

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Sugar non-specific endonucleases are multifunctional enzymes and are widespread in distribution. Apart from nutrition, they have also been implicated in cellular functions like replication, recombination and repair. Their ability to recognize different DNA structures has also been exploited for the determination of nucleic acid structure. Although more than 30 non-specific endonucleases have been isolated to date, very little information is available regarding their structure^function correlations except that of staphylococcal and Serratia nucleases. However, during the past few years, the primary structure, nature of the active site based on sequence homology, and the probable mechanism of action have been postulated for some of the enzymes. This review describes the purification, characteristics, biological role and applications of sugar non-specific endonucleases. ß

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Endo-deoxyribonuclease from Streptomyces rimosus

Vukelić

Ritonja

Vitale

1995

Appl Microbiol Biotechnol

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From filtrates of an oxytetracycline-producing culture of Streptomyces rimosus a deoxyribonuclease was purified to homogeneity and determined to be a potent endo-DNase. It is a monomeric, basic protein (M(r) approximately 21,000; pI approximately 9.5) stable in a broad pH range but unstable to higher temperature. The enzyme has an absolute requirement for Mg2+ or Mn2+, and for its full activity requires free SH groups and a low-ionic-strength environment. Its N-terminal primary structure differs from that of other nucleases.

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Shrimp hepatopancreatic deoxyribonuclease — purification and characterization as well as comparison with bovine pancreatic deoxyribonuclease

Cited by 17 publications

References 36 publications

A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas

A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas

Sugar non-specific endonucleases

Endo-deoxyribonuclease from Streptomyces rimosus

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