Should we worry about the clock? Relationship between time to ICSI and reproductive outcomes in cycles with fresh and vitrified oocytes

Quintana-Bárcena, Patricia; Rodriguez, Maria B; Obradors, Albert; Vernaeve, V.; Vassena, Rita

doi:10.1093/humrep/dew070

Cited by 29 publications

(10 citation statements)

References 25 publications

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“…Other studies have found no significant differences in reproductive outcomes across a wide time interval range between oocyte retrieval and ICSI [31]. A recent study in which time intervals between OPU and ICSI ranged from 1 h 25 min to 17 h 13 min revealed no significant difference in reproductive outcomes including biochemical, ongoing and live pregnancy rate [32]. In our protocols, rescue ICSI was performed 6 h after initial insemination and about 10 h after OPU.…”

Section: Discussionmentioning

confidence: 58%

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

Xiong

Sun

et al. 2020

BMC Pregnancy Childbirth

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Background: Early rescue intracytoplasmic sperm injection (ICSI) has been used in clinic as appropriate currently. While the outcomes of children born after this method were not well assessed. The purpose of this study was to evaluate the effect of early rescue ICSI on women with primary infertility. Methods: Fresh embryo transfer cycles after rescue (n = 214) and conventional (n = 546) ICSI were retrospectively evaluated from women with primary infertility who underwent their first assisted reproductive technology cycles at our center in 2012-2017. The conventional ICSI group was subdivided into ICSI-1 (semen suitable for in vitro fertilization, IVF) and ICSI-2 (poor semen quality) to minimize bias from differences in semen quality. Pregnancy, delivery and neonatal outcomes were compared between groups. Results: There was a higher rate of polyspermy and a lower rate of top-quality embryos (TQE) on day 3 for oocytes subject to rescue ICSI compared with conventional ICSI. This reduced the total number of TQE and the number of TQE transferred in the rescue ICSI group. There was no significant difference between groups in clinical pregnancy, ongoing pregnancy, early miscarriage and live birth. For pregnant women, gestational age, route of delivery, risk of preterm birth and gestational diabetes mellitus were also comparable. Neonatal outcomes including sex ratio, birth weight, neonatal intensive care unit admission and birth defects were also similar after rescue and conventional ICSI. Moreover, no differences were observed with the different ICSI subgroups. Conclusions: For women with primary infertility who have a high risk of IVF fertilization failure (FF), rescue ICSI provides a safe and efficient alternative to minimize FF after initial IVF, but results in fewer TQE on day 3.

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Section: Discussionmentioning

confidence: 58%

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

Xiong

Sun

et al. 2020

BMC Pregnancy Childbirth

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show abstract

“…Second, to better isolate the sole effect of sperm incubation time on reproductive outcomes, we performed logistic regression, in which adjusting variables like male and female age, day of ET and the morphological score of transferred embryos were introduced, all of them well‐described to affect pregnancy and LB (McPherson et al, 2018; Rhenman et al, 2015). In addition, the OPU–ICSI time was also added to adjust the analysis in autologous ICSI cycles, as this time interval is reported to affect this kind of treatment but not ICSI cycles with donor oocytes (Barcenas et al, 2016; Pujol et al, 2018). The logistic regression identified a weak association between T and LB rate which is very close to the level of statistical significance.…”

Section: Discussionmentioning

confidence: 99%

“…However, more precise timekeeping would allow for a more accurate dissection of the effect of sperm incubation time on reproductive outcomes. The use of radiofrequency‐based witnessing systems to record exact, operator‐independent processing times, has allowed to describe the variable effect of oocyte in vitro ageing on reproductive outcomes after ICSI (Barcenas et al, 2016; Pujol et al, 2018). By using this same technology on a large cohort of patients, we aim now to elucidate whether sperm incubation times affect laboratory and reproductive outcomes after ICSI.…”

Section: Introductionmentioning

confidence: 99%

How long can the sperm wait? Effect of incubation time on ICSI outcomes

Torra‐Massana¹,

Quintana-Vehí²,

Barragán³

et al. 2022

Molecular Reproduction Devel

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In sperm processing for IVF/ICSI incubation times differ considerably both between and within assisted reproduction facilities. There is no established consensus on the optimal sperm incubation timings to maximize pregnancy rates, and the few studies addressing this association rely on manual and operator-dependent methods for time recording. The present retrospective cohort study includes 1169 ICSI cycles using fresh semen processed by swim-up. An operator-independent, radiofrequency-based system was used to record sperm incubation times: from sample collection to swim-up (T1, 0.35 ± 0.26); from swim-up to ICSI (T2, 3.30 ± 2.2); and total time from sample collection to ICSI (T, 3.66 ± 2.26). In oocyte donation cycles, we observed a significant negative effect of T1 on fertilization rate (FR; generalized linear modelling regression, coeff. −0.20, p = 0.001); however, after analysing all times by deciles and by adjusted logistic regression, none of the time intervals had a significant effect on pregnancy (biochemical, clinical, and ongoing) and live birth (LB) rates (p > 0.05 for all outcomes). In cycles using the patient's oocytes, we observed a negative effect of T2 (ordinal regression, coeff. −0.25, p = 0.011) and T (−0.33, p = 0.005) on the mean morphological score of the embryo cohort. In these cycles, a trend associating longer values of T with higher LB rates was identified (OR = 1.47, p = 0.050), although this difference is likely not clinically significant. In conclusion, while longer sperm incubation in vitro may impact slightly both FRs and embryo morphology after ICSI, no adverse effects were detected on the reproductive outcomes.

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“…All oocytes were inseminated by intracytoplasmic sperm injection (ICSI) 2-3 hours after warming. We chose this time for ICSI as it has been reported that most functions, such as meiotic spindle recovery, mitochondria activity and ATP level recovery in frozen/warmed oocytes, take about 2-3 hours after warming [29][30][31] and it has been found that ICSI time (9 ± 2 h) after oocyte retrieval in the vitrified human oocytes does not affect clinical outcomes [32].…”

Section: Insemination Fertilization Assessment Embryo Culture and Fresh Blastocyst Transfermentioning

confidence: 99%

Efficiency of Autologous Egg Cryopreservation: Eight Years’ Experiences and Clinical Outcomes

Wang¹,

Niu²,

Ruan³

2021

Infertility and Assisted Reproduction

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Oocyte cryopreservation is one of the state-of-art technologies in human reproductive medicine, which brings opportunities for women to preserve their fertility. In the present study, we analyzed the efficiency and outcomes of 8 years’ autologous egg cryopreservation: Frozen oocytes were warmed from 120 cycles and oocyte survival, fertilization, blastocyst development, clinical pregnancy, embryo implantation, live birth rates and birth weights were collected based on the patients’ ages of <35, 35–37 and > 37 years old. The details of oocyte cryopreservation and the efficiency were further analyzed based on different patient categories. During the study period, 849 oocytes from 120 cycles were warmed. Oocyte survival, fertilization, and blastocyst development were not affected by women’s ages at the time of cryopreservation. However, number of patients without blastocyst formation was significantly (P < 0.05) higher in patients >37 years old (31.2%) than that in patients <35 years old (13.1%). Higher live birth rates were observed in patients <35 (51.1%) and 35–37 (46.7%) years old than in patients >37 years old (28.6%) after fresh embryo transfer. Some patients did not have blastocysts mainly due to low fertilization by poor sperm or small number of oocytes warmed. These results indicate that the efficiency of oocyte cryopreservation, evaluated by live birth and embryo implantation rates is affected by women’s age, number of oocytes warmed and sperm quality.

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Should we worry about the clock? Relationship between time to ICSI and reproductive outcomes in cycles with fresh and vitrified oocytes

Cited by 29 publications

References 25 publications

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

How long can the sperm wait? Effect of incubation time on ICSI outcomes

Efficiency of Autologous Egg Cryopreservation: Eight Years’ Experiences and Clinical Outcomes

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