Should we test hepatitis C virus genotype and viraemia level in patients with chronic hepatitis C?

Isaacson, A. H.; Davis, Gary L.; Lau, Johnson Y.N.

doi:10.1046/j.1365-2893.1997.00065.x

Cited by 9 publications

(8 citation statements)

References 47 publications

(13 reference statements)

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“…Some HCV genotypes, particularly 1b, are considered to be more virulent and more resistant to treatment by interferon-␣ [Dusheiko and Simmonds, 1994b;Dusheiko et al, 1996;Bruno et al, 1997;Isaacson et al, 1997;Kiyosawa, 1997;Mihm et al, 1997;Pawlotsky et al, 1998]. Continued surveillance will monitor spread of possibly more virulent and drugresistant HCV genotypes.…”

Section: Discussionmentioning

confidence: 99%

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The most prevalent hepatitis C virus genotypes in England and Wales are 3a and 1a

Harris

Gilham²,

Mortimer

et al. 1999

J. Med. Virol.

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Hepatitis C virus (HCV) genotypes were assigned to 567 individuals by restriction fragment length polymorphism analysis of the 5' noncoding region of the HCV genome following reverse transcription-polymerase chain reaction. The groups of individuals in this study included hemophilia patients, injecting drug users (IDUs), blood donors, antenatal patients, those attending genitourinary medicine (GUM) clinics, and patients with chronic liver disease, all from England and Wales. The majority of HCV infections were types 1a (32%), 1b (15%), or 3a (37%). The genotype distribution in individual groups was similar to the overall genotype distribution except for hemophilia patients, in whom the frequencies were 1a (39%), 1b (23%), and 3a (21%). With the exception of hemophilia patients, subpopulations in England and Wales appear to share common modes of HCV transmission. There is a need for continued surveillance to monitor the spread of possibly more virulent or drug-resistant HCV genotypes.

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Section: Discussionmentioning

confidence: 99%

“…Different genotypes may carry different clinical significance Bruno et al, 1997;Kiyosawa, 1997;Mihm et al, 1997]. They may also influence response to interferon-alpha, currently the only therapeutic drug licensed for the treatment of HCV infection Dusheiko et al, 1996;Isaacson et al, 1997;Kiyosawa, 1997].…”

Section: Introductionmentioning

confidence: 99%

The most prevalent hepatitis C virus genotypes in England and Wales are 3a and 1a

Harris

Gilham²,

Mortimer

et al. 1999

J. Med. Virol.

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“…As serum HCV load is not per se a useful indicator in the selection of patient candidates for liver biopsy, we suggest that biopsy should be routinely performed in all subjects with PNAL (4, 6), as it remains the only means to delineate the presence and the degree of liver injury in these patients. Further long-term studies will be needed to evaluate the role of viremia in the management of patients with chronic HCV infection (59), and to establish whether the natural history of chronic HCV infection in "asymptomatic" HCV carriers with higher HCV RNA titer is different from those with a lower degree of viral replication.…”

Section: Discussionmentioning

confidence: 99%

Serum HCV RNA titer does not predict the severity of liver damage in HCV carriers with normal aminotransferase levels

Puoti¹,

Stati²,

Magrini³

1999

Liver Int

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In HCV carriers with normal aminotransferase levels viraemia does not predict the grade of HCV-related chronic liver disease (CLD), although subjects with higher HCV RNA levels seem to have more severe fibrosis. Although these data suggest that patients with higher viraemia might have more intense architectural changes and more severe progression of liver disease than those with lower levels of HCV replication, the weak and imprecise correlation leads us to conclude that HCV RNA quantitation is not a useful indicator in clinical practice in the selection of patients for liver biopsy.

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“…These genotyping results can be useful in viral transmission studies as well as in epidemiological investigations. Furthermore, in many studies, associations between viral genotype, interferon responsiveness, the progression of disease, and the likelihood of developing hepatocellular carcinoma have been demonstrated (6,11,17,18,21,22,34,38,39). Thus, although the basis for these associations is poorly understood, HCV genotyping is being performed increasingly in clinical studies of HCV patients.…”

mentioning

confidence: 99%

Determination of Hepatitis C Virus Genotype by Direct Sequence Analysis of Products Generated with the Amplicor HCV Test

et al. 1999

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Consistent with other members of the familyFlaviviridae, hepatitis C virus (HCV) demonstrates a high degree of sequence variation throughout the coding regions of its genome. However, there is a high degree of sequence conservation found within the 5′ untranslated region (UTR) of the genome, making this region a target of choice for most nucleic acid amplification-based detection assays. In this study, the Amplicor HCV test, a commercially available assay which detects the 5′UTR, was used for the detection of HCV RNA in 669 serum samples obtained from a cohort of liver transplantation patients. Amplification products obtained from the HCV-positive cases were subjected to direct sequencing and genotyping based upon seven phylogenetically informative regions within the 5′UTR. Of the 669 specimens, 416 (62.2%) tested positive for the presence of HCV RNA. Of these, 372 (89.4%) specimens were successfully classified into 11 HCV genotypes and subtypes after computer-assisted analysis of the sequence data. Forty-four (10.6%) of the HCV RNA-positive specimens were not classifiable, the majority corresponding to low-titer specimens as determined by the Chiron Quantiplex HCV RNA 2.0 assay. Additional comparative studies targeting the NS-5 region of the viral genome generally confirmed the accuracy and sensitivity of the 5′UTR-based classifications, with the exception of the misclassification of a small number of type 1a cases as type 1b. We conclude that although the high sequence conservation within the 5′UTR results in the misclassification of a small number of HCV subtypes, the overall gains of efficiency, the shorter turnaround time, the inclusion of contamination control measures, and the low rate of test failure compared to that of methods based on the NS-5 gene together constitute significant advantages over other techniques.

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Should we test hepatitis C virus genotype and viraemia level in patients with chronic hepatitis C?

Cited by 9 publications

References 47 publications

The most prevalent hepatitis C virus genotypes in England and Wales are 3a and 1a

The most prevalent hepatitis C virus genotypes in England and Wales are 3a and 1a

Serum HCV RNA titer does not predict the severity of liver damage in HCV carriers with normal aminotransferase levels

Determination of Hepatitis C Virus Genotype by Direct Sequence Analysis of Products Generated with the Amplicor HCV Test

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