Shotgun proteomics deciphered age/division of labor-related functional specification of three honeybee (Apis mellifera L.) exocrine glands

Fujita, Toshiyuki; Kozuka‐Hata, Hiroko; Hori, Yutaro; Takeuchi, Jun; Kubo, Takeo; Oyama, Masaaki

doi:10.1371/journal.pone.0191344

Cited by 5 publications

(6 citation statements)

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“…In our previous study (Wu et al 2017), the expression level of CYP6AS8 in MGs of worker was over 100-fold higher compared to the MGs of queens and was selectively expressed in MG compared to other tissues. Furthermore, proteomic research on honey bee exocrine glands has confirmed our findings at the protein level (Fujita et al 2018), suggesting that CYP6AS8 are involved in the hydroxylation of MG secretions.…”

Section: Discussionsupporting

confidence: 82%

CYP6AS8, a cytochrome P450, is associated with the 10-HDA biosynthesis in honey bee (Apis mellifera) workers

Zheng

Li-Byarlay

et al. 2020

Apidologie

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Mandibular gland (MG) secretions of honey bee are synthesized through a three-step bifurcation pathway, and the initiate hydroxylation step is carried out by cytochrome P450 enzymes (P450). We have previously revealed that CYP6AS8 and CYP6AS11 are possibly participating in this process. In this study, we evaluated the function of CYP6AS8 in honey bee worker's MG using RNA interference technology, and we investigated the expression pattern of CYP6AS8 in MGs from workers of different ages. We found that the expression of CYP6AS8 was detectable in MGs across the whole life cycle of Apis mellifera except for newly emerged workers and predominately expressed at day 15. RNAi knockdown revealed that the production of 10-HDA was synchronized with the expression level of CYP6AS8 in MG of honey bee workers; the downregulation of CYP6AS8 caused a significant reduction of 10-HDA content, demonstrating that CYP6AS8 is indispensable for 10-HDA biosynthesis. Our current results confirm that CYP6AS8 modulates 10-HDA biosynthesis in MGs of western honey bee worker and provided the first evidence to identify the certain P450 participate in the MG secretion biosynthesis of honey bee workers.cytochrome P450 / mandibular gland / biosynthesis / CYP6AS8

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Section: Discussionsupporting

confidence: 82%

CYP6AS8, a cytochrome P450, is associated with the 10-HDA biosynthesis in honey bee (Apis mellifera) workers

Zheng

Li-Byarlay

et al. 2020

Apidologie

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“…For SCP‐2 in the MDGs, this is confirmed by the proteomic study of Huo et al (2016) who detect the protein in MDGs of newly emerged bees, nurses and foragers with the highest abundance in newly emerged bees (gi|571540960 and gi|571540964 [Huo et al, 2016]). Another MDG proteome study confirms the presence of SCP‐2 protein in nurses and foragers (XP_392432.2 [Fujita et al, 2018]). SCP‐2 was further identified via mass spectrometry in the heads of 21‐day‐old caged worker honey bees that were fed a 24MC‐supplemented diet (0.1%–1% w / w ) but the protein was not found to be significantly different between 24MC‐supplemented and 24MC‐free diet (A0A088A9R3 [Chakrabarti & Sagili, 2020]).…”

Section: Discussionmentioning

confidence: 86%

“…In the HPGs, SCP-2 is identified at the protein level in newly emerged bees, nurses, and foragers (gi|328790232, GeneID 408904 [Dobritzsch et al, 2019;Fujita et al, 2013;Hu et al, 2019]). None of the proteome studies identifies NPC1a at the protein level in the MDGs or the HPGs (Dobritzsch et al, 2019;Fujita et al, 2013Fujita et al, , 2018Hu et al, 2019;Huo et al, 2016). However, NPC1a is a transmembrane protein and membrane proteins are difficult to analyze with mass spectrometry because of their amphipathic nature (Mirza et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…For MDGs, the transcriptome data by Wu et al (2017) reveal 33‐times more mapped reads for queens than for workers indicating that sterol storage may not be present, or minor, in workers but might be relevant in queens. Whereas ACAT was indeed not detected in proteome studies on MDGs of workers of different ages (Fujita et al, 2018; Huo et al, 2016), the single proteome study on queen MDGs concentrated on odorant‐binding and chemosensory proteins and ACAT was not among the 24 other most abundant proteins identified by two‐dimensional gel electrophoresis (Iovinella et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Expression of honey bee (Apis mellifera) sterol homeostasis genes in food jelly producing glands of workers

Yusuf,

Pirk,

Buttstedt

2024

J Exp Zool Pt A

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Adult workers of Western honey bees (Apis mellifera L.) acquire sterols from their pollen diet. These food sterols are transported by the hemolymph to peripheral tissues such as the mandibular and the hypopharyngeal glands in the worker bees' heads that secrete food jelly which is fed to developing larvae. As sterols are obligatory components of biological membranes and essential precursors for molting hormone synthesis in insects, they are indispensable to normal larval development. Thus, the study of sterol delivery to larvae is important for a full understanding of honey bee larval nutrition and development. Whereas hypopharyngeal glands only require sterols for their membrane integrity, mandibular glands add sterols, primarily 24‐methylenecholesterol, to its secretion. For this, sterols must be transported through the glandular epithelial cells. We have analyzed for the first time in A. mellifera the expression of genes which are involved in intracellular movement of sterols. Mandibular and hypopharyngeal glands were dissected from newly emerged bees, 6‐day‐old nurse bees that feed larvae and 26‐day‐old forager bees. The expression of seven genes involved in intracellular sterol metabolism was measured with quantitative real‐time PCR. Relative transcript abundance of sterol metabolism genes was significantly influenced by the age of workers and specific genes but not by gland type. Newly emerged bees had significantly more transcripts for six out of seven genes than older bees indicating that the bulk of the proteins needed for sterol metabolism are produced directly after emergence.

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“…Mandibular glands of honeybees have been found to have a high potential to produce lipids [41]. Glucose oxidase, produced in the mandibular glands, has been found in propolis [42,43].…”

Section: Effect Of Surface Coating On Propolis Adhesionmentioning

confidence: 99%

Interaction between honeybee mandibles and propolis

Saccardi¹,

Brümmer²,

Schiebl³

et al. 2022

Beilstein J. Nanotechnol.

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In a biomimetic top-down process, challenging the problem of resin deposition on woodworking machine tools, an adequate biological model was sought, which hypothetically could have developed evolutionary anti-adhesive strategies. The honeybee (Apis mellifera) was identified as an analogue model since it collects and processes propolis, which largely consists of collected tree resin. Propolis is a sticky substance used by bees to seal their hive and protect the colony against pathogens. In spite of its stickiness, honeybees are able to handle and manipulate propolis with their mandibles. We wanted to know if beneficial anti-adhesive properties of bee mandibles reduce propolis adhesion. The anatomy of bee mandibles was studied in a (cryo-)scanning electron microscope. Adhesion experiments were performed with propolis on bee mandibles to find out if bee mandibles have anti-adhesive properties that enable bees to handle the sticky material. A scale-like pattern was found on the inside of the mandible. Fresh mandibles were covered with a seemingly fluid substance that was at least partially removed during the washing process. Propolis adhesion on bee mandibles was measured to be 1 J/m2 and was indeed significantly lower compared to five technical materials. Propolis adhesion was higher on mandibles that were washed compared to fresh, unwashed mandibles. Results indicate that the medial surface of the mandible is covered with a fluid substance that reduces propolis adhesion. First results suggested that the surface pattern does do not have a direct effect on propolis adhesion.

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Shotgun proteomics deciphered age/division of labor-related functional specification of three honeybee (Apis mellifera L.) exocrine glands

Cited by 5 publications

References 61 publications

CYP6AS8, a cytochrome P450, is associated with the 10-HDA biosynthesis in honey bee (Apis mellifera) workers

CYP6AS8, a cytochrome P450, is associated with the 10-HDA biosynthesis in honey bee (Apis mellifera) workers

Expression of honey bee (Apis mellifera) sterol homeostasis genes in food jelly producing glands of workers

Interaction between honeybee mandibles and propolis

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