Shotgun proteome analysis of honeybee venom using targeted enrichment strategies

Matysiak, Jan; Hajduk, Joanna; Pietrzak, Łukasz; Schmelzer, Christian E.H.; Kokot, Zenon J.

doi:10.1016/j.toxicon.2014.08.069

Cited by 27 publications

(21 citation statements)

References 57 publications

(60 reference statements)

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“…Serine carboxypeptidase is an exopeptidase that is a major allergen in honeybee venom [47]. It is known to participate in protein digestion in the guts of animals [48] and is found among the yolk proteins in mosquito oocytes [49].…”

Section: Expression Patterns Of Other Venom Proteinsmentioning

confidence: 99%

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Yoon

Kim

Kim³

et al. 2020

Toxins

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To identify and compare venom components and expression patterns, venom gland-specific transcriptome analyses were conducted for 14 Aculeate bees and wasps. TPM (transcripts per kilobase million) values were normalized using the average transcription level of a reference housekeeping gene (dimethyladenosine transferase). Orthologous venom component genes across the 14 bee and wasp species were identified, and their relative abundance in each species was determined by comparing normalized TPM values. Based on signal sequences in the transcripts, the genes of novel venom components were identified and characterized to encode potential allergens. Most of the allergens and pain-producing factors (arginine kinase, hyaluronidase, mastoparan, phospholipase A1, phospholipase A2, and venom allergen 5) showed extremely high expression levels in social wasps. Acid phosphatase, neprilysin, and tachykinin, which are known allergens and neurotoxic peptides, were found in the venom glands of solitary wasps more often than in social wasps. In the venom glands of bumblebees, few or no transcripts of major allergens or pain-producing factors were identified. Taken together, these results indicate that differential expression patterns of the venom genes in some Aculeate species imply that some wasps and bumblebee species have unique groups of highly expressed venom components. Some venom components reflected the Aculeate species phylogeny, but others did not. This unique evolution of specific venom components in different groups of some wasps and bumblebee species might have been shaped in response to both ecological and behavioral influences.

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Section: Expression Patterns Of Other Venom Proteinsmentioning

confidence: 99%

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Yoon

Kim

Kim³

et al. 2020

Toxins

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“…Low-abundance molecules remained undescribed for decades thus tackling our systemic comprehension of the envenoming process. Proteomic and peptidomic analyses based on the use of techniques such as LC-ESI-MS and nano-ESI-MS/MS have progressively reversed this situation enabling identification of thousands of novel venom proteins and peptides [24,47,48].…”

Section: Exploring P Paulista Venom -Omics Approachesmentioning

confidence: 99%

“…Also, the large number of Hymenoptera proteins with unknown functions deposited in databases when the analysis was conducted hampered the identification of other venom toxins [57,58]. A reanalysis of the venom proteome of P. paulista using a gel-free shotgun proteomic approach combined with the use of updated protein databases could significantly enhance the identification of wasp venom toxins [48,64] with relevant biological functions. The use of this sensitive analytical alternative will allow the identification of the low-abundance "hidden proteins" in P. paulista venom thus helping to complete the model proposed for the envenoming process [48].…”

Section: Exploring P Paulista Venom -Omics Approachesmentioning

confidence: 99%

“…A reanalysis of the venom proteome of P. paulista using a gel-free shotgun proteomic approach combined with the use of updated protein databases could significantly enhance the identification of wasp venom toxins [48,64] with relevant biological functions. The use of this sensitive analytical alternative will allow the identification of the low-abundance "hidden proteins" in P. paulista venom thus helping to complete the model proposed for the envenoming process [48]. Proteinaceous compounds identified using shotgun proteomics could be also evaluated for the development of novel drugs with potential pharmaceutical applications.…”

Section: Exploring P Paulista Venom -Omics Approachesmentioning

confidence: 99%

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Wasp venomic: Unravelling the toxins arsenal of Polybia paulista venom and its potential pharmaceutical applications

Pérez-Riverol

Santos-Pinto

Lasa

et al. 2017

Journal of Proteomics

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Polybia paulista (Hymenoptera: Vespidae) is a neotropical social wasp from southeast Brazil. As most social Hymenoptera, venom from P. paulista comprises a complex mixture of bioactive toxins ranging from low molecular weight compounds to peptides and proteins. Several efforts have been made to elucidate the molecular composition of the P. paulista venom. Data derived from proteomic, peptidomic and allergomic analyses has enhanced our understanding of the whole envenoming process caused by the insect sting. The combined use of bioinformatics, -omics- and molecular biology tools have allowed the identification, characterization, in vitro synthesis and recombinant expression of several wasp venom toxins. Some of these P. paulista - derived bioactive compounds have been evaluated for the rational design of antivenoms and the improvement of allergy specific diagnosis and immunotherapy. Molecular characterization of crude venom extract has enabled the description and isolation of novel toxins with potential biotechnological applications. Here, we review the different approaches that have been used to unravel the venom composition of P. paulista. We also describe the main groups of P. paulista - venom toxins currently identified and analyze their potential in the development of component-resolved diagnosis of allergy, and in the rational design of antivenoms and novel bioactive drugs.

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“…There are a lot of previous studies about the presence of peptides and enzymes in HBV [16][17][18] but there are very few papers on the content of low-molecular-weight compounds. Otherwise, in the available literature regarding the content of small molecules in animal venoms, authors rely on old papers, so there is a lack of source information and current research that could confirm the found data.…”

Section: Introductionmentioning

confidence: 99%

Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids

Pawlak

Klupczynska

Kokot

et al. 2019

Toxins

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Organic acids are important active small molecules present in venoms and toxins, which have not been fully explored yet. The aim of the study was the determination of organic acids in honeybee venom (HBV) samples by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two protocols for sample preparation were employed. A solid-phase extraction was used for the determination of malonic acid, fumaric acid, glutaric acid, and kynurenic acid. A dilute-and-shoot method was optimal for: citric acid, malic acid, and succinic acid. Chromatographic separation was performed using a Synergi Hydro-RP column. Detection was performed on a triple-quadrupole mass spectrometer operating in multiple reaction monitoring mode. Among the analytes, glutaric acid and kynurenic acid were present in HBV samples in the lowest concentrations, whereas citric acid was the most abundant acid in each sample, and accounted for an average of 86 mg/g (8.6%) of the venom dry weight. Organic acids were discussed in terms of function. This is the first study in the available literature that provides specific data on the content of organic acids in HBV using a validated quantitative method.Key Contribution: The first study that provided specific and accurate data on the content of a panel of low-molecular-weight organic acids in honeybee venom.

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Shotgun proteome analysis of honeybee venom using targeted enrichment strategies

Cited by 27 publications

References 57 publications

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Wasp venomic: Unravelling the toxins arsenal of Polybia paulista venom and its potential pharmaceutical applications

Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids

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