Short wave ultraviolet laser energy in porcine coronary arteries: Medial cell death and neointimal formation

Grégoire, Jean; Edwards, William D.; Jeong, Myung Ho; Camrud, Allan R.; Lerman, Amir; Tassel, Robert A. Van; Bailey, Kent R.; Holmes, David R.; Schwartz, Robert S.

doi:10.1002/(sici)1096-9101(1997)21:4<374::aid-lsm9>3.0.co;2-n

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…The average lifetimes of typical standard dyes ͑long, medium, and short lifetimes͒ were found in good agreement with the values reported in the literature ͑Table I͒. [24][25][26] The data are reported as meanϮ standard error of ten repeated measurements. Commercially available samples of collagen type I from bovine Achilles tendon ͑C-9879, Sigma-Aldrich, St. Louis, MO͒ and elastin from human aorta ͑E-6777, Sigma-Aldrich͒ were tested in dry powder form.…”

Section: A Tr-lifs Componentssupporting

confidence: 86%

Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy

Sun

Park

Stephens

et al. 2009

Review of Scientific Instruments

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We report a tissue diagnostic system which combines two complementary techniques of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) and ultrasonic backscatter microscopy (UBM). TR-LIFS evaluates the biochemical composition of tissue, while UBM provides tissue microanatomy and enables localization of the region of diagnostic interest. The TR-LIFS component consists of an optical fiber-based time-domain apparatus including a spectrometer, gated multichannel plate photomultiplier, and fast digitizer. It records the fluorescence with high sensitivity (nM concentration range) and time resolution as low as 300 ps. The UBM system consists of a transducer, pulser, receiving circuit, and positioning stage. The transducer used here is 45 MHz, unfocused, with axial and lateral resolutions 38 and 200 microm. Validation of the hybrid system and ultrasonic and spectroscopic data coregistration were conducted both in vitro (tissue phantom) and ex vivo (atherosclerotic tissue specimens of human aorta). Standard histopathological analysis of tissue samples was used to validate the UBM-TRLIFS data. Current results have demonstrated that spatially correlated UBM and TR-LIFS data provide complementary characterization of both morphology (necrotic core and calcium deposits) and biochemistry (collagen, elastin, and lipid features) of the atherosclerotic plaques at the same location. Thus, a combination of fluorescence spectroscopy with ultrasound imaging would allow for better identification of features associated with tissue pathologies. Current design and performance of the hybrid system suggests potential applications in clinical diagnosis of atherosclerotic plaque.

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Section: A Tr-lifs Componentssupporting

confidence: 86%

Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy

Sun

Park

Stephens

et al. 2009

Review of Scientific Instruments

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“…UV‐induced cell death has also been demonstrated in arteries in vivo. Pig coronary arteries irradiated with a 275 nm laser showed acellular media as a result of death of SMC (5). These reported cell deaths were induced by UV light that is in the range of UV‐C (200–290 nm), whereas the present results were obtained from irradiation of UV light at a wavelength of 313 nm, which is in the range of UV‐B (290–320 nm).…”

Section: Discussionmentioning

confidence: 99%

“…Some studies using animal models have demonstrated that UV irradiation prevents restenosis or vasospasm. The media of pig coronary arteries were rendered acellular after exposure to UV light (275 nm) of a dose of >13 mJ/mm 2 irradiated via an optical fiber through a balloon catheter (5). Vasospasms in rabbit carotid arteries were successfully prevented by treatment with UV light (325 nm) at a dose of 10 mJ/mm 2 , indicating that apoptosis may be involved in the mechanism of this prophylactic effect (6).…”

Section: Introductionmentioning

confidence: 99%

Long-term Effects of UV Light on Contractility of Rat Arteries In Vivo¶

Morimoto

Kohyama

Nakai

et al. 2007

Photochemistry and Photobiology

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Several studies have shown that UV irradiation may be effective for preventing vascular restenosis or vasospasm. However, the long‐term effects of UV light on the physiological properties of vessels such as arterial tension have not been elucidated. We therefore studied the long‐term effects of UV using rat carotid arteries treated with UV‐B light (wavelength = 313 nm, total energy = 14 mJ/mm2). The animals were sacrificed at 1, 7 and 14 days after UV light exposure, and the carotid arteries were studied by light microscopy and the contractile responses of isolated arterial rings were recorded under isometric tension. UV treatment had induced a substantial loss of smooth muscle cells (SMC) along the entire circumference of the media on days 7 and 14, whereas loss of SMC on day 1 was negligible. Contractile responses of arteries that had been exposed to UV light were significantly reduced on days 1, 7 and 14. The susceptibility of UV‐treated arteries to phenylephrine and prostaglandin F2α was significantly decreased on days 1 and 7, but decreased susceptibility was not seen on day 14. Acetylcholine‐induced relaxations were not altered by UV treatment. These results suggest that the long‐term effect of UV light is an attenuation of smooth muscle contractility without impairment of endothelial function.

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“…Reduction in proliferative capacity has been observed in vascular cells treated with UV-C (wavelength <290 nm) irradiation [7]. Media of pig coronary arteries were rendered acellular after exposure to UV-C (275 nm) at a dose of > 13 mJ/mm 2 irradiated via an optical fiber through a balloon catheter [8]. Psoralen plus ultraviolet-A light (PUVA (wavelength ¼ 320-400 nm) irradiation) has been shown to reduce even vascular intimal hyperplasia after angioplasty [9,10].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of narrow‐band ultraviolet‐B phototherapy for prevention of intimal hyperplasia in a rat carotid balloon injury model

et al. 2007

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Short wave ultraviolet laser energy in porcine coronary arteries: Medial cell death and neointimal formation

Cited by 8 publications

References 28 publications

Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy

Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy

Long-term Effects of UV Light on Contractility of Rat Arteries In Vivo¶

Effectiveness of narrow‐band ultraviolet‐B phototherapy for prevention of intimal hyperplasia in a rat carotid balloon injury model

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