Short-term UV-B exposure induces metabolic and anatomical changes in peel of harvested lemons contributing in fruit protection against green mold

Ruiz, Verónica Eugenia; Interdonato, Roque; Cerioni, Luciana; Albornoz, Patricia Liliana; Ramallo, J. C.; Prado, Fernando Eduardo; Hilal, Mirna; Rapisarda, Viviana Andrea

doi:10.1016/j.jphotobiol.2016.03.016

Cited by 30 publications

(16 citation statements)

References 34 publications

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“…Leaf cell walls in fas1 and fas2 mutants increase their thickness after UV‐B exposure, these changes in cell wall properties may also raise their UV‐B absorption properties, and as a consequence, absorbance by cell wall components may protect the cells against DNA damage. Some plant cell wall components, such as phenolic compounds, have been previously demonstrated to act as UV‐screening molecules, increasing tolerance against UV‐B (Machinandiarena, Oyarburo, Daleo, Andreu, & Olivieri, ; Pontin et al, ; Ruiz et al, ). Moreover, our results show that CAF‐1 mutants accumulate higher levels of soluble UV‐B absorbing compounds, similarly as what is was previously shown in det1 mutants, which have altered chromatin architecture and accumulate higher UV sunscreen compounds (Castells et al, ).…”

Section: Discussionmentioning

confidence: 99%

AtCAF‐1 mutants show different DNA damage responses after ultraviolet‐B than those activated by other genotoxic agents in leaves

Maulión

Gómez

Bustamante

2019

Plant Cell & Environment

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Chromatin assembly factor-1 (CAF-1) is a histone H3/H4 chaperone that participates in DNA and chromatin interaction processes. In this manuscript, we show that organs from CAF-1 deficient plants respond differently to ultraviolet-B (UV-B) radiation than to other genotoxic stresses. For example, CAF-1 deficient leaves tolerate better UV-B radiation, showing lower cyclobutane pyrimidine dimer (CPD) accumulation, lower inhibition of cell proliferation, increased cell wall thickness, UV-B absorbing compounds, and ploidy levels, whereas previous data from different groups have shown that CAF-1 mutants show shortening of telomeres, loss of 45S rDNA, and increased homologous recombination, phenotypes associated to DNA breaks. Interestingly, CAF-1 deficient roots show increased inhibition of primary root elongation, with decreased meristem size due to a higher inhibition of cell proliferation after UV-B exposure. The decrease in root meristem size in CAF-1 mutants is a consequence of defects in programmed cell death after UV-B exposure. Together, we provide evidence demonstrating that root and shoot meristematic cells may have distinct protection mechanisms against CPD accumulation by UV-B, which may be linked with different functions of the CAF-1 complex in these different organs.

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Section: Discussionmentioning

confidence: 99%

AtCAF‐1 mutants show different DNA damage responses after ultraviolet‐B than those activated by other genotoxic agents in leaves

Maulión

Gómez

Bustamante

2019

Plant Cell & Environment

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“…However, after 36 h of UV-B exposure, the concentration of such phenolics significantly increased. In the study byRuiz et al (2016), a significantly higher concentration of several flavonoid subclasses (flavanones, dihydroflavonols, flavones, flavonols and anthocyanins) was detected 48 h after 3 min UV-B treatment in lemon skin.…”

mentioning

confidence: 88%

“…radiation (Hagen et al, 2007;Ruiz et al, 2016;Scattino et al, 2014), we first checked possible change in their profile to verify whether and how such metabolites were modulated by the UV-B treatments. To this aim, a phenolics-specific database (Phenol-Explorer) was used to identify the compounds resulting from the UHPLC-ESI/QTOF-MS analysis.…”

Section: Influence Of Uv-b Treatments On Phenolic Profilementioning

confidence: 99%

Post-harvest UV-B radiation modulates metabolite profile in peach fruit

Santin

Lucini

Castagna

et al. 2018

Postharvest Biology and Technology

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The possibility to modify plant metabolic profile of plants and fruit to improve their healthy properties using eco-friendly tools, rather than transgenic approaches, gained interest in the last decades. Ultraviolet-B (UV-B) radiation, at low levels, thanks to its ability to influence plant secondary metabolism, could be successfully used to achieve this goal. However, few studies have been conducted so far on the effects of post-harvest UV-B treatments on fruit metabolomics. The present research, aimed to evaluate the impact of UV-B on peach metabolites profile through nontargeted metabolomics (UHPLC-ESI/QTOF-MS) coupled with multivariate chemometrics, provided evidence that 10 and 60 min of post-harvest UV-B irradiation influenced several classes of metabolites. Most phenolics were downaccumulated 24 h after both UV-B treatments, though, after 36 h, anthocyanins, flavones and dihydroflavonols increased (2.06-, 1.92-, 1.68-fold with 10 min UV-B; 6.65-, 2.53-, 2.05-fold with 60 min UV-B, respectively). UV-B reduced carotenoids and most lipids and increased some biosynthetic intermediates and degradation products, some of them known for their positive role in human health. Among alkaloids, some pteridines accumulated, likely derived from folates degradation, while indole alkaloids decreased. Despite the decrease of some bioprotective metabolites as carotenoids, the UV-B-induced up-accumulation of many antioxidant phenolics after 36 h from the exposure suggests an improvement of the healthy properties of peach fruit and reinforces the potential of UV-B controlled irradiation as a nutraceuticals-increasing tool in fruit.

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“…It was reported that, lemon fruits irradiated by UV‐B leads to thickening of the cell walls, thus reinforcing barrier against pathogenic fungi. UV treatment promotes the synthesis of antifungal secondary metabolites, such as phytoalexins and polyphenols, which can limit the infection of pathogenic fungi (Ruiz et al., 2016; Ruiz et al., 2017). UV‐C radiation exerts its antifungal activities by promoting phytochemical reactions; these are influenced by the duration and temperature of incubation (Usall et al., 2016).…”

Section: Recent Strategies For Securing Fruit Productionmentioning

confidence: 99%

Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections

Ngea

Qian

Yang

et al. 2021

Comp Rev Food Sci Food Safe

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Fruit‐based diets have been adopted by the public worldwide because of their nutritional value. Many advances have also been made in the elucidation of host–pathogen interaction in the postharvest phase of fruits, in the hope of improving the management of diseases caused by pathogenic molds. In this study, we presented the molecular mechanisms by which pathogenic mold infects fruit in the postharvest phase, and focused on the knowledge gained from recent molecular techniques such as differential analysis of gene expression, targeted insertion, and mutagenesis. Current postharvest pathogenic fungal control strategies were then examined on the basis of their mechanisms for altering the infection process in order to explore new perspectives for securing fruit production. We found that biotechnological advances have led to an understanding of the new basic molecular processes involved in fruit fungal infection and to the identification of new genes, proteins and key factors that could serve as ideal targets for innovative antifungal strategies. In addition, the most commonly used steps to evaluate an approach to disrupt the fruit fungal infection process are mainly based on the inhibition of mycelial growth, spore germination, disruption of Adenosine triphosphate (ATP) synthesis, induction of oxidative stress, cell wall membrane damage, and inhibition of key enzymes. Finally, the alteration of the molecular mechanisms of signaling and response pathways to infection stimulation should also guide the development of effective control strategies to ensure fruit production.

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Short-term UV-B exposure induces metabolic and anatomical changes in peel of harvested lemons contributing in fruit protection against green mold

Cited by 30 publications

References 34 publications

AtCAF‐1 mutants show different DNA damage responses after ultraviolet‐B than those activated by other genotoxic agents in leaves

AtCAF‐1 mutants show different DNA damage responses after ultraviolet‐B than those activated by other genotoxic agents in leaves

Post-harvest UV-B radiation modulates metabolite profile in peach fruit

Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections

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