Short term temperature acclimation in myofibrillar ATPase of a stenotherm Salmo gairdneri Richardson and an eurytherm Carassius auratus

Abstract

Myofibrillar ATPase activity was measured in the epaxial musculature of rainbow trout acclimated to 5 and 2 1 a C, and goldfish acclimated to 5 and 30" C. The acclimated goldfish showed considerable differences in the specific activity and thermostability of this enzyme.However, such alterations were not apparent in the acclimated trout. The evolutionary implications of these findings are discussed.

Temperature acclimation in the rockfishAcanthoclinus quadridactylus

“…This has been shown for the goldfish (Johnston et al 1975b, Sidell 1980, Goldspink & Penney 1982, in conjunction with changes in key metabolic enzymes (Jones & Sidell 1982, Tsukuda 1982. ATPase isoenzymes are not produced in the stenothermal trout (Penney & Goldspink 1981) although trout do display some adaptations to temperature in the gills (Sellner & Hazel 1982), and also in metabolic enzymes in skeletal muscle (Hazel & Prosser 1974). In the present study, myofibrillar ATPase activity did not change because of acclimation to different temperatures.…”

“…Study of these isoenzymes can gIve mformatIOn about the ability of a fish to acclimatise t.o a range ~f temperatures and the enzyme myo~bnllar ade~osme triphosphatase (ATPase), the protem responSIble for splitting A TP in skeletal muscle to provide energy for contraction has received much attention. Stenothermal fish a;e not known to produce ATPase isoenzymes (Penney & Goldspink 1981), whereas the eurythermal goldfish has at ~east 2 (Johnston et al 197 5b), and probably more, Isoenzymes which function at high and low Downloaded by [North Dakota State University] at 09:27 13 October 2014 temperatures (Penney & Goldspink 1979, Goldspink & Penney 1982.…”

Consequences of thermal change on the myofibrillar ATPase of five freshwater teleosts

“…The preparation of myofibrils was a further modification of that used by Penney & Goldspink (1981b), based on the method of Perry & Grey (1956). The white epaxial musculature was dissected and immersed in 3 volumes of ice-cold 0.1 M KCl, 5 mM Tris-HC1, 1 mM dithiothreitol, pH 7.0 (extraction buffer).…”