Short-term storage of Persian sturgeon (Acipenser persicus) ova in artificial media and coelomic fluid

Abstract

The influence of short-term storage (3, 6 and 9 h) of Persian sturgeon (Acipenser presicus) ova outside the ovary (ex situ storage) in coelomic fluid and PSACF (Persian Sturgeon Artificial Coelomic Fluid) medium was studied at (4°C and 18°C). One mature female and three mature males were selected for gamete collection and fertilization, as well as 18 females were sampled for coelomic fluid collection. Fish were intramuscularly injected with acetone-dried sturgeon pituitary and their ova and milt collected according to common spawning methods. Thereafter ova were filtered and coelomic fluid was separated for further using in experiment on ova storage. For mimicking of ovarian fluid and designing the PSACF medium, chemical composition of ovarian fluid from 18 females was analyzed separately in 26 samples and osmolarity and pH for each sample were determined and the PSACF medium was formulated.This experiment was done with 12 treatments in 3 replicate using four factor combinations. For each treatment 5 cc (about 300) ova were used. After storage for 3, 6 and 9 h in the two test temperature, ova were fertilized with pooled sperm and then transferred to Yoshchenko incubators. Fertilization rate, hatching rate and percentage of malformed larvae were determined.Storag period and storage medium had significant effect, on fertilization rate, hatching success and malformation of larvae (P<0.05). Also, temperature had significant effects on fertilization rate (P<0.05) but no significant effects on rate of hatching and number of malformed larvae (P 0.05). Fertilization and hatching rates decreased with time (3, 6 and 9 hours of storage prior to fertilization). Malformation rates in hatched larvae increased compared to the controls. Interactions between time of storage and storage medium and temperature and the resulting effects on fertilization, hatching and malformation rates were significant (P<0.05).Using a prepared PSACF medium at 18ºC for 3 h resulted in 80.33% fertilization rate, 70.5% hatching rate and 2.6% malformation rate in newly hatched Persian sturgeon larvae.

Improvement of gamete quality and its short-term storage: an approach for biotechnology in laboratory fish

“…In general, warm-water species presents low storage ability after ovulation (Rizzo et al, 2003;Nguenga et al, 2004). Better storage are found in some cold-water species including salmonids (Barrett, 1951;Withler and Morley, 1968;Poon and Johnson, 1970;Jensen and Alderdice, 1984;Babiak and Dabrowski, 2003;Niksirat et al, 2007), sturgeons (Billard et al, 2004;Sohrabnezhad et al, 2006), flatfishes (Suquet et al, 1999) and walleye (Dietrich et al, 2012) although it is dependent on species and storage conditions (Rothbard et al, 1996;Linhart et al, 2001;Yasui et al, 2010). Based on our results, we conclude that oocytes from yellowtail tetra cannot be stored successfully, although other procedures may be used in future works including solutions with additives that may improve storage ability as was observed in the other warm-water fish such as the tilapia (Harvey and Kelley, 1984).…”

Optimization Protocol for Storage of Goldfish (Carassius auratus) Embryos in Chilled State

“…; Sohrabnezhad et al. ). In our study, chilling of goldfish embryos in GFACF and Dettlaff's solution instead of water and other physiological saline solutions prolonged their viability.…”

Comparative coelomic fluid composition of sterlet sturgeon Acipenser ruthenus Linnaeus, 1758, Siberian sturgeon Acipenser baerii Brandt, 1869, and Russian sturgeon Acipenser gueldenstaedtii Brandt & Ratzeburg, 1833

“…The ionic compositions of ovarian fluid in salmonids showed similar ion concentration ranges except for Na + , which was different (Hatef et al., ; Lahnsteiner et al., ). The concentrations of Na + and Ca ++ were lower, while K + , Mg + , Cl − and osmotic pressure were higher in the CF of Persian sturgeon Acipenser persicus (Sohrabnezhad et al., ). Beside species‐specific differences, such variances in the composition of CF can be related to changes in a female's physiological status throughout the spawning season, as well as with changes in egg quality and maturity (Aegerter & Jalabert, ; Lahnsteiner, ; Lahnsteiner, Weismann, & Patzner, ).…”

“…The results of the present study may be useful to develop some artificial media for washing and short‐term storage of sturgeon eggs, which would be useful for transportation to the hatchery of gametes after stripping to synchronize insemination (Linhart et al., ; Suquet et al., ). Several artificial media were developed based on the ionic composition of body fluids and successfully used to store unfertilized eggs of rainbow trout ( Oncorhynchus mykiss ), Persian sturgeon ( Acipenser persicus ), and brown trout ( Salmo trutta ; Goetz & Coffman, ; Niksirat, Sarvi, Mojazi Amiri, & Hatef, ; Niksirat, Sarvi, Mojazi Amiri, Karami et al., ; Sohrabnezhad et al., ). Washing and short‐term storage of unfertilized eggs in such artificial media can increase eyeing and hatching rates (Goetz & Coffman, ).…”

“…Ovarian fluid protects the oocytes from physiological damage and dryness during both in vivo and in vitro conditions. Numerous studies have shown that CF can influence the fertilization rate by preserving fertilization ability of the eggs during short‐term storage (Jia, Niu, Meng, Liu, & Lei, ; Sohrabnezhad, Kalbassi, Nazari, & Bahmani, ). Ovarian fluid is discharged with eggs during spawning and can affect motility traits of spermatozoa (Butts, Johnson, Wilson, & Pitcher, ; Dietrich et al., ; Litvak & Trippel, ; Turner & Montgomerie, ; Wojtczak et al., ).…”