Short-term stimulation of lipogenesis by triiodothyronine in maintenance cultures of rat hepatocytes

Gnoni, Gabriele V.; Geelen, M.J.H.; Bijleveld, Caspaar; Quagliariello, Ernesto; Bergh, S.G. van den

doi:10.1016/0006-291x(85)90078-6

Cited by 32 publications

(27 citation statements)

References 18 publications

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“…Nuclear Run-on Assays-Nuclear run-on assays were performed as described (28). The in vitro elongation reaction was initiated with the addition of 0.25 mmol/liter each of ATP, GTP, CTP, and UTP for 25 min at 30°C.…”

Section: Methodsmentioning

confidence: 99%

MDA-9/Syntenin Is Essential for Factor VIIa-induced Signaling, Migration, and Metastasis in Melanoma Cells

Aissaoui

Prévost

Boucharaba

et al. 2015

Journal of Biological Chemistry

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Background: MDA-9/syntenin and tissue factor (TF) are overexpressed in most types of human cancer. Results: Induction of MDA-9/syntenin in melanoma involves the binding of FVIIa and FX to TF on the cell surface, which initiates a signaling circuit essential for cell motility and metastasis of melanoma. Conclusion: MDA-9/syntenin is an important TF-regulated gene. Significance: Targeting TF-mediated MDA-9/syntenin may represent a novel therapeutic strategy for eliminating cancer.

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Section: Methodsmentioning

confidence: 99%

MDA-9/Syntenin Is Essential for Factor VIIa-induced Signaling, Migration, and Metastasis in Melanoma Cells

Aissaoui

Prévost

Boucharaba

et al. 2015

Journal of Biological Chemistry

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“…23 Briefly; nuclei were isolated from Caco2-BBE cells treated with or without 40 ng/ml of TNF-␣ for 6 hours. Approximately 5 ϫ 10 7 cells were pelleted in a conical tube and washed twice with ice-cold PBS.…”

Section: Nuclear Run-on Assaymentioning

confidence: 99%

Nuclear Factor-κB Is a Critical Mediator of Ste20-Like Proline-/Alanine-Rich Kinase Regulation in Intestinal Inflammation

Yan

Dalmasso

Nguyen

et al. 2008

The American Journal of Pathology

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“…Nuclei were isolated from hepatocytes obtained by liver perfusion and collagenase digestion as reported by Gnoni et al [17], washed twice with cold PBS (phosphate-buffered saline) and then lysed as in [12]. The crude nuclei were purified as described by Siculella et al [12] and nuclear run-on assay was carried out as described by Liu et al [18].…”

Section: Isolation Of Nuclei and Nuclear Run-on Assaymentioning

confidence: 99%

Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver

et al. 2004

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The tricarboxylate carrier (TCC), an integral protein of the mitochondrial inner membrane, transports mitochondrial acetyl-CoA into the cytosol, where lipogenesis occurs. We investigated in rat liver mitochondria the effect of diets enriched with saturated fatty acids (beef tallow, BT), monounsaturated fatty acids (olive oil, OO) or n À 3 polyunsaturated fatty acids (fish oil, FO), respectively, on the activity and expression of TCC. TCC activity decreased, in parallel with TCC mRNA abundance, only upon FO-feeding. The TCC transcription rate, mRNA turnover and RNA processing indicated that FO administration regulates TCC gene at transcriptional and post-transcriptional steps, whereas BT-and OO-feeding do not seem to affect either TCC activity or gene expression.

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Short-term stimulation of lipogenesis by triiodothyronine in maintenance cultures of rat hepatocytes

Cited by 32 publications

References 18 publications

MDA-9/Syntenin Is Essential for Factor VIIa-induced Signaling, Migration, and Metastasis in Melanoma Cells

MDA-9/Syntenin Is Essential for Factor VIIa-induced Signaling, Migration, and Metastasis in Melanoma Cells

Nuclear Factor-κB Is a Critical Mediator of Ste20-Like Proline-/Alanine-Rich Kinase Regulation in Intestinal Inflammation

Different dietary fatty acids have dissimilar effects on activity and gene expression of mitochondrial tricarboxylate carrier in rat liver

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