Short-Term Reloading After Prolonged Unloading Ensures Restoration of Stromal but Not Hematopoietic Precursor Activity in Tibia Bone Marrow of C57Bl/6N Mice

Markina, E. A.; Alekseeva, Olga Yu; Андреева, Е.Р.; Buravkova, Ludmila

doi:10.1089/scd.2021.0111

Cited by 1 publication

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In HU, a decrease in hindlimb bone mass in mice and a decrease in bone mass and osteoblast number in rats [16] were described. The in vitro experiments revealed decreased proliferative activity of BM stromal cells, decreased number of CFU-f and inhibition of osteopotential of stromal precursors [25]. After HU, a downregulation of transcripts of osteocalcin (OS), bone sialoprotein (BSP), alkaline phosphatase (ALP) family proteins, bone morphogenetic protein 1 (BMP1), and stromal cell factor SDF-1/CXCL12 in stromal precursors from mouse BM were demonstrated [26].…”

Section: Discussionmentioning

confidence: 99%

Stromal Lineage Precursors from Rodent Femur and Tibia Bone Marrows after Hindlimb Unloading: Functional Ex Vivo Analysis

Markina

Andreeva

Буравкова

2023

IJMS

Self Cite

View full text Add to dashboard Cite

Rodent hindlimb unloading (HU) model was developed to elucidate responses/mechanisms of adverse consequences of space weightlessness. Multipotent mesenchymal stromal cells (MMSCs) were isolated from rat femur and tibia bone marrows and examined ex vivo after 2 weeks of HU and subsequent 2 weeks of restoration of load (HU + RL). In both bones, decrease of fibroblast colony forming units (CFU-f) after HU with restoration after HU + RL detected. In CFU-f and MMSCs, levels of spontaneous/induced osteocommitment were similar. MMSCs from tibia initially had greater spontaneous mineralization of extracellular matrix but were less sensitive to osteoinduction. There was no recovery of initial levels of mineralization in MMSCs from both bones during HU + RL. After HU, most bone-related genes were downregulated in tibia or femur MMSCs. After HU + RL, the initial level of transcription was restored in femur, while downregulation persisted in tibia MMSCs. Therefore, HU provoked a decrease of osteogenic activity of BM stromal precursors at transcriptomic and functional levels. Despite unidirectionality of changes, the negative effects of HU were more pronounced in stromal precursors from distal limb—tibia. These observations appear to be on demand for elucidation of mechanisms of skeletal disorders in astronauts in prospect of long-term space missions.

show abstract

Section: Discussionmentioning

confidence: 99%