Short-term perfusion and ?Equilibration? of canine kidneys with protective solutions

Kallerhoff, M.; Blech, M.; Kehrer, G.; Kleinert, H.; Langheinrich, Matthias; Siekmann, W.; Helmchen, Udo; Bretschneider, H. J.

doi:10.1007/bf00256327

Cited by 8 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Compared with canine kidneys which were perfused with the same technique, the same solution and the same pressure, and showed a medium perfusion resistance of 0.3 mmHg/ml per minute x 100 g wet weight in previous studies [12,14], the perfusion resistance of the human kidneys was almost 2.8 times higher. However, perfusion resistances of up to 0.6 mmHg/ml per minute × 100 g wet weight also occurred occasionally in animal experiments [18].…”

Section: Discussionmentioning

confidence: 93%

“…On the other hand, there was complete equilibration for potassium both in animal experiments and in human kidneys [14]. The equilibration in the perfusion urine was slower than in the venous effluence.…”

Section: Discussionmentioning

confidence: 95%

“…In previous animal experiments [14], there was practically no warm ischemia before the beginning of the perfusion. However, warm ischemia times cannot be avoided in our investigation model.…”

Section: Discussionmentioning

confidence: 97%

“…At a high perfusion resistance, on the other hand, the equilibration is delayed. However, complete equilibration is a crucial prerequisite for successful protection, since only in this way can the protective solution be completely effective [14,16]. Since the human kidneys showed a higher perfusion resistance in this investigation model compared with canine kidneys in previous studies, delayed and/or incomplete equilibration may also occur.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Electrolyte equilibration of human kidneys during perfusion with HTK-solution according to Bretschneider

et al. 1997

Self Cite

View full text Add to dashboard Cite

Twelve surgically removed human kidneys (mainly tumor kidneys) were investigated. The investigations comprised perfusion criteria (perfusion flow, perfusion pressure, perfusion resistance, electrolyte equilibration). During perfusion of the kidneys with HTK solution, the perfusion resistance was nearly three times as high in human kidneys as in canine kidneys perfused under the same conditions in previous studies. Beside possible species differences the raised perfusion resistance may be explained by the greater trauma to the human kidneys due to the surgery, the primary ischemic stress which cannot be avoided clinically and the often nonoptimal initial diuresis. Nevertheless definitive perfusion is possible under clinical conditions although pronounced increases of perfusion resistance may occur. As indicated by the raised perfusion resistance of human kidneys under clinical conditions as compared with canine kidneys in an experimental model, electrolyte equilibration of human kidneys was protracted. For this reason, a duration of perfusion of at least 10 min is necessary in clinical application of HTK solution, i.e., longer than in animal experiments.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Electrolyte equilibration of human kidneys during perfusion with HTK-solution according to Bretschneider

et al. 1997

Self Cite

View full text Add to dashboard Cite

show abstract

“…la, 2a, [3][4][5]. In biopsies of beating hearts fixed by immersion, bundles of myocytes arc sepa rated by small to slightly enlarged tissue clefts (Vvrc = 8.1 ± 1.0%).…”

Section: Qualitative and Morphometric Data O F The Interstitial And Imentioning

confidence: 99%

Influence of Pretreatment on Interstitial and Intracellular Space of Canine Left-Ventricular Myocardium

Schmiedl¹,

Schnabel²,

Haasis³

et al. 1990

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

In this study, the interstitial space and myocytes were investigated qualitatively and morphometrically in samples from beating, fibrillating as well as from cardioplegically HTK-arrested hearts fixed by immersion or perfusion. The size of tissue clefts separating bundles of myocytes and that of the interstitial space within bundles of myocytes depend on the functional state and on the kind of fixation. Cellular preservation is significantly better in HTK-arrested hearts compared to beating or fibrillating hearts. Thus, for the structural evaluation of myocytes and interstitium, the pretreatment constitutes a highly significant factor.

show abstract

A new method for conservative renal surgery ? experimental and first clinical results

Kallerhoff

Blech

Götz

et al. 1990

Langenbecks Arch Chir

Self Cite

View full text Add to dashboard Cite

So far two methods for prolonging the tolerance of renal ischemia are available: 1) surface cooling with crushed ice and 2) perfusion cooling with an extracellular-like solution. Both methods use only the principle of reducing metabolism through cooling. While rewarming during surgery the ischemic protection is lost, or the kidney must be cooled once again. Therefore, a new preservation solution should reduce energy consumption due to its composition in addition to cooling. For open heart surgery, the HTK solution by Bretschneider is already used clinically. In 71 dog kidney experiments, the ischemic time kidneys could tolerate was prolonged by this solution from 15 to 120 min at 35 degrees C and from 45 to 360 min at 25 degrees C. After 2 h of ischemia at 30 degrees C glomerular filtration rate was about 20 ml/min.100gww within 3 h of reperfusion. After six postoperative days the filtration rate was 40 ml/min.100 gww. No ischemic damage could be recognized by histological investigations. The clinical effectiveness of this method was shown in 7 clinical applications. Ischemic duration lasted up to 113 min, and blood creatinine was between 0.8 and 2.4 mg% at the 6th postoperative day. Use of this preservation technique thus leads to improved kidney function immediately following operation. Longer ischemia can be tolerated by a kidney thus protected, and using this technique excellent visibility can be achieved during intrarenal surgery, simplifying, for example, tumor extirpation.

show abstract

Short-term perfusion and ?Equilibration? of canine kidneys with protective solutions

Cited by 8 publications

References 23 publications

Electrolyte equilibration of human kidneys during perfusion with HTK-solution according to Bretschneider

Electrolyte equilibration of human kidneys during perfusion with HTK-solution according to Bretschneider

Influence of Pretreatment on Interstitial and Intracellular Space of Canine Left-Ventricular Myocardium

A new method for conservative renal surgery ? experimental and first clinical results

Contact Info

Product

Resources

About