Short-term particulate matter exposure induces extracellular vesicle release in overweight subjects

Bonzini, Matteo; Pergoli, Laura; Cantone, Laura; Hoxha, Mirjam; Spinazzè, Andrea; Buono, Luca Del; Favero, Chiara; Carugno, Michele; Angelici, Laura; Broggi, L; Cattaneo, Andrea; Pesatori, Angela Cecilia; Bollati, Valentina

doi:10.1016/j.envres.2017.02.014

Cited by 32 publications

(20 citation statements)

References 33 publications

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“…As studies of EVs are still in their infancy, it is impossible to draw any definitive conclusion on this complex relationship between PM exposure, EVs, and BMI. One possible scenario is that normal-weight subjects show a low reactivity to PM exposure, as shown in our previous work [ 34 ], whereas overweight subjects acquire higher reactivity to PM. All of the EV types investigated are positively modulated by PM exposure, which is suggestive of a complex interplay and cross-talk between different cell types.…”

Section: Discussionmentioning

confidence: 98%

“…These findings are consistent with our preliminary investigation in 25 normal-weight subjects (BMI < 25) and 25 overweight subjects (25 ≤ BMI < 30) who were exposed to PM 10 and PM 2.5 . The effect of PM exposure on EV release was visible in overweight subjects only [ 34 ] and was mainly focused on EV of endothelial origin (CD105+).…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, in the abovementioned paper by Bonzini et al, we compared PM 10 and PM 2.5 levels measured by ambient monitoring stations with those measured through personal samplers in 51 healthy volunteers, and found the two measures to be significantly correlated ( r = 0.59, P < 0.001). Furthermore, the two pollutants showed a highly significant correlation ( r = 0.97; P < 0.001) [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

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Extracellular vesicle-packaged miRNA release after short-term exposure to particulate matter is associated with increased coagulation

Pergoli¹,

Cantone²,

Favero³

et al. 2017

Part Fibre Toxicol

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BackgroundExposure to particulate matter (PM) is associated with increased incidence of cardiovascular disease and increased coagulation, but the molecular mechanisms underlying these associations remain unknown. Obesity may increase susceptibility to the adverse effects of PM exposure, exacerbating the effects on cardiovascular diseases. Extracellular vesicles (EVs), which travel in body fluids and transfer microRNAs (miRNAs) between tissues, might play an important role in PM-induced cardiovascular risk. We sought to determine whether the levels of PM with an aerodynamic diameter ≤ 10 μm (PM10) are associated with changes in fibrinogen levels, EV release, and the miRNA content of EVs (EV-miRNAs), investigating 1630 overweight/obese subjects from the SPHERE Study.ResultsShort-term exposure to PM10 (Day before blood drawing) was associated with an increased release of EVs quantified by nanoparticle tracking analysis, especially EVs derived from monocyte/macrophage components (CD14+) and platelets (CD61+) which were characterized by flow cytometry. We first profiled miRNAs of 883 subjects by the QuantStudio™ 12 K Flex Real Time PCR System and the top 40 EV-miRNAs were validated through custom miRNA plates. Nine EV-miRNAs (let-7c-5p; miR-106a-5p; miR-143-3p; miR-185-5p; miR-218-5p; miR-331-3p; miR-642-5p; miR-652-3p; miR-99b-5p) were downregulated in response to PM10 exposure and exhibited putative roles in cardiovascular disease, as highlighted by integrated network analysis. PM10 exposure was significantly associated with elevated fibrinogen levels, and five of the nine downregulated EV-miRNAs were mediators between PM10 exposure and fibrinogen levels.ConclusionsResearch on EVs opens a new path to the investigation of the adverse health effects of air pollution exposure. EVs have the potential to act both as markers of PM susceptibility and as potential molecular mechanism in the chain of events connecting PM exposure to increased coagulation, which is frequently linked to exposure and CVD development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12989-017-0214-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Extracellular vesicle-packaged miRNA release after short-term exposure to particulate matter is associated with increased coagulation

Pergoli¹,

Cantone²,

Favero³

et al. 2017

Part Fibre Toxicol

Self Cite

View full text Add to dashboard Cite

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“…PM exposure was found to increase EV release by cultured endothelial cells (Neri et al 2016). Observational studies in healthy volunteers provided evidence that high levels of outdoor air pollution or PM occurred concomitantly with elevated concentrations of endothelial-derived EV, especially in subjects that were overweight (Bonzini et al 2017;Pope et al 2016). …”

Section: Induction Of Endothelial Extracellular Vesicles By Respiratomentioning

confidence: 98%

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Benedikter

Wouters

Savelkoul

et al. 2018

Journal of Toxicology and Environmental Health, Part B

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Extracellular vesicles (EV) are secreted signaling entities that enhance various pathological processes when released in response to cellular stresses. Respiratory exposures such as cigarette smoke and air pollution exert cellular stresses and are associated with an increased risk of several chronic diseases. The aim of this review was to examine the evidence that modifications in EV contribute to respiratory exposure-associated diseases. Publications were searched using PubMed and Google Scholar with the search terms (cigarette smoke OR tobacco smoke OR air pollution OR particulate matter) AND (extracellular vesicles OR exosomes OR microvesicles OR microparticles OR ectosomes). All original research articles were included and reviewed. Fifty articles were identified, most of which investigated the effect of respiratory exposures on EV release in vitro (25) and/or on circulating EV in human plasma (24). The majority of studies based their main observations on the relatively insensitive scatter-based flow cytometry of EV (29). EV induced by respiratory exposures were found to modulate inflammation (19), thrombosis (13), endothelial dysfunction (11), tissue remodeling (6), and angiogenesis (3). By influencing these processes, EV may play a key role in the development of cardiovascular diseases and chronic obstructive pulmonary disease and possibly lung cancer and allergic asthma. The current findings warrant additional research with improved methodologies to evaluate the contribution of respiratory exposure-induced EV to disease etiology, as well as their potential as biomarkers of exposure or risk and as novel targets for preventive or therapeutic strategies.

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“…Short-term PM exposure is associated with an increased release of EVs 12,13,24 , which are considered to be important mediators of the cross-talk between cells 10 . A growing body of evidence shows that the effects of PM exposure is associated with endothelial dysfunction, suggesting that EVs may be important players in this mechanism 12,13 . In the present study, we investigated the impact of plasmatic EVs isolated from healthy donors on endothelial primary cells.…”

Section: Discussionmentioning

confidence: 99%

Blood-derived extracellular vesicles isolated from healthy donors exposed to air pollution modulate in vitro endothelial cells behavior

Rota

Ferrari

Hoxha

et al. 2020

Sci Rep

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The release of Extracellular Vesicles (EVs) into the bloodstream is positively associated with Particulate Matter (PM) exposure, which is involved in endothelial dysfunction and related to increased risk of cardiovascular disease. Obesity modifies the effects of PM exposure on heart rate variability and markers of inflammation, oxidative stress, and acute phase response. We isolated and characterized plasmatic EVs from six healthy donors and confirmed a positive association with PM exposure. We stratified for Body Mass Index (BMI) and observed an increased release of CD61+ (platelets) and CD105+ (endothelium) derived-EVs after high PM level exposure in Normal Weight subjects (NW) and no significant variations in Overweight subjects (OW). We then investigated the ability to activate endothelial primary cells by plasmatic EVs after both high and low PM exposure. NW-high-PM EVs showed an increased endothelial activation, measured as CD105+/CD62e+ (activated endothelium) EVs ratio. On the contrary, cells treated with OW-high-PM EVs showed reduced endothelial activation. These results suggest the ability of NW plasmatic EVs to communicate to endothelial cells and promote the crosstalk between activated endothelium and peripheral cells. However, this capacity was lost in OW subjects. Our findings contribute to elucidate the role of EVs in endothelial activation after PM exposure.

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Short-term particulate matter exposure induces extracellular vesicle release in overweight subjects

Cited by 32 publications

References 33 publications

Extracellular vesicle-packaged miRNA release after short-term exposure to particulate matter is associated with increased coagulation

Extracellular vesicle-packaged miRNA release after short-term exposure to particulate matter is associated with increased coagulation

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Blood-derived extracellular vesicles isolated from healthy donors exposed to air pollution modulate in vitro endothelial cells behavior

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