Short-Term In-Vitro Sensitivity Testing of Human Renal Cell Carcinoma

Riese, Werner de; Szűcs, Sándor; Hoene, Eberhard; Lenis, Gustavo; Kovács, Gyula; Schindler, E.

doi:10.1007/978-3-642-72735-1_13

Cited by 6 publications

(3 citation statements)

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“…The monolayer procedure according to Kovacs et al [16] provides an in vitro success rate for RCC of nearly 90%. For routine application this method proved valuable in producing slides with high cell density, and additionally preserves material and cells [23], thus allowing simultaneous assays to be performed which are particularily suitable for in vitro drug sensitivity studies as presented herein. Reliable interpretation of tumor cell cultures essentially depends on the positive identification of malignancy and the purity of the tumor cell population as well [12,22].…”

Section: Discussionmentioning

confidence: 99%

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In vitro sensitivity testing of human renal cell carcinoma with cytostatic agents and interferon alpha-2a

et al. 1991

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Summary. Samples of 38 human renal cell carcinomas(RCC) were subjected to routine histopathological examination but also to in vitro sensitivity testing with mitomycin C, vinblastine and interferon Alpha-2a at various concentrations corresponding to serum titers recommended to be effective in vivo, employing a monolayer assay. Extending earlier in vitro studies, both tumor cell kill rates (TCKR) and proliferation rates (PR) were assessed. Following in vitro preparation the tumor cell cultures were simultaneously exposed to the anticancer drugs listed above. The proliferation rates were determined immunocytochemically using the monoclonal antibody Ki-67. Nine (23.7%) of the tumors investigated revealed temporary and limited response with respect to either TCKR or PR. Improvement of this percentage could only be obtained by increasing drug concentration to titers with toxicity intolerable for in vivo administration. The in vivo data presented correspond to clinical temporary and limited remissions in patients with metastatic RCC ranging up to 25 %.

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Section: Discussionmentioning

confidence: 99%

“…This was considered the starting point for cell counting to obtain the TCKR comparing the number of treated cells with that of untreated cells [23].…”

Section: Short-term In Vitro Sensitivity Testingmentioning

confidence: 99%

In vitro sensitivity testing of human renal cell carcinoma with cytostatic agents and interferon alpha-2a

et al. 1991

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“…1 erläutert als graphische Darstellung die gewählte Antikörperverknüpfung, der detaillierte Ablauf der einzelnen Arbeitsschritte ist in Tab. 1 wiedergegeben: Bei Vorhanden- 3 2 3 3 2 3 2 1 2 3 3 2 2 3 2 2 2 2 3 1 2 3 2 2 2 G 2 1 3 3 2 3 2 1 2 2 1 1 1 3 2 2 2 2 2 1 3 2 1 2 3 2 3 Parallel zur In-vivo-Proliferationsbestimmung präparierten wir jeweils den Tumor auch in-vitro, wie erst kürzlich von unserer Arbeitsgruppe beschrieben (18). Für einen Tumor gelang die Etablierung einer Zellinie mit bisher über 40 Passagen.…”

Section: Materials Und Methodeunclassified