Short-Term Hypothermia Activates Hepatic Mitochondrial sn-Glycerol-3-Phosphate Dehydrogenase and Thermogenic Systems

Bobyleva, Valentina; Pazienza, Loredana; Muscatello, Umberto; Kneer, Nancy; Lardy, Henry A.

doi:10.1006/abbi.2000.1942

Cited by 14 publications

(11 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This enzyme is responsible for transforming glycerol in dihydroxyacetone phosphate and for producing reduced equivalents to the respiratory chain, during lipolysis, and could also contribute to thermogenesis (Lardy et al 1995, Koza et al 1996, Bobyleva et al 2000, dos Santos et al 2003. So, we can suggest that the lower liver mGPD activity could be related to the hypometabolism observed in these animals (Bonomo et al 2007).…”

Section: Discussionmentioning

confidence: 93%

Prolactin inhibition at the end of lactation programs for a central hypothyroidism in adult rat

Bonomo

Lisboa²,

Passos³

et al. 2008

Journal of Endocrinology

View full text Add to dashboard Cite

Malnutrition during lactation is associated with hypoprolactinemia and failure in milk production. Adult rats whose mothers were malnourished presented higher body weight and serum triiodothyronine (T 3 ). Maternal hypoprolactinemia at the end of lactation caused higher body weight in adult life, suggesting an association between maternal prolactin (PRL) level and programming of the offspring's adult body weight. Here, we studied the consequences of the maternal PRL inhibition at the end of lactation by bromocriptine (BRO) injection, a dopaminergic agonist, upon serum TSH and thyroid hormones, thyroid iodide uptake, liver mitochondrial a-glycerophosphate dehydrogenase (mGPD), liver and pituitary de-iodinase activities (D1 and/or D2), and in vitro post-TRH TSH release in the adult offspring. Wistar lactating rats were divided into BRO -injected with 1 mg/twice a day, daily for the last 3 days of lactation, and C -control, saline-injected with the same frequency. At 180 days of age, the offspring were injected with 125 I i.p. and after 2 h, they were killed. Adult animals whose mothers were treated with BRO at the end of lactation presented lower serum TSH (K51%), T 3 (K23%), and thyroxine (K21%), lower thyroid 125 I uptake (K41%), liver mGPD (K55%), and pituitary D2 (K51%) activities, without changes in the in vitro post-TRH TSH release. We show that maternal PRL suppression at the end of lactation programs a hypometabolic state in adulthood, in part due to a thyroid hypofunction, caused by a central hypothyroidism, probably due to decreased TRH secretion. We suggest that PRL during lactation can regulate the hypothalamus-pituitary-thyroid axis and programs its function.

show abstract

Section: Discussionmentioning

confidence: 93%

Prolactin inhibition at the end of lactation programs for a central hypothyroidism in adult rat

Bonomo

Lisboa²,

Passos³

et al. 2008

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…The liver has a known thermogenic role in animals living in the cold. This has been demonstrated either by a direct response to cold stimuli through an increase in its heat production (Baconnier et al, 1979;Bobyleva et al, 2000;Dewasmes et al, 2003) or as an elevation in its oxidative capacity (Goglia et al, 1993;Villarin et al, 2003). Villarin et al even argued that this organ could play an active thermogenic role during acute cold stress and has the potential to generate as much as 44% of the total heat produced during M sum in cold acclimated marsupial Monodelphis domestica (Villarin et al, 2003).…”

Section: Does Cold Acclimation Induce Higher Maintenance Costs?mentioning

confidence: 99%

Acclimation to different thermal conditions in a northerly wintering shorebird is driven by body mass-related changes in organ size

Vézina

Jalvingh

Dekinga

et al. 2006

Journal of Experimental Biology

117

109

View full text Add to dashboard Cite

show abstract

“…Moreover, flight muscle mitochondria have high activities of mGPDH, which is also highly expressed in BAT and has been suggested to have a thermogenic function in mammals 14–17 . To sustain glycolytic flux, the glycerol 3-phosphate (G3P) pathway effectively oxidises cytosolic NADH.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial glycerol 3-phosphate facilitates bumblebee pre-flight thermogenesis

Masson

Hedges

Devaux

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Bumblebees (Bombus terrestris) fly at low ambient temperatures where other insects cannot, and to do so they must pre-warm their flight muscles. While some have proposed mechanisms, none fully explain how pre-flight thermogenesis occurs. Here, we present a novel hypothesis based on the less studied mitochondrial glycerol 3-phosphate dehydrogenase pathway (mGPDH). Using calorimetry, and high resolution respirometry coupled with fluorimetry, we report substrate oxidation by mGPDH in permeabilised flight muscles operates, in vitro, at a high flux, even in the absence of ADP. This may be facilitated by an endogenous, mGPDH-mediated uncoupling of mitochondria. This uncoupling increases ETS activity, which results in increased heat release. Furthermore, passive regulation of this mechanism is achieved via dampened temperature sensitivity of mGPDH relative to other respiratory pathways, and subsequent consumption of its substrate, glycerol 3-phosphate (G3P), at low temperatures. Mitochondrial GPDH may therefore facilitate pre-flight thermogenesis through poor mitochondrial coupling. We calculate this can occur at a sufficient rate to warm flight muscles until shivering commences, and until flight muscle function is adequate for bumblebees to fly in the cold.

show abstract

Short-Term Hypothermia Activates Hepatic Mitochondrial sn-Glycerol-3-Phosphate Dehydrogenase and Thermogenic Systems

Cited by 14 publications

References 27 publications

Prolactin inhibition at the end of lactation programs for a central hypothyroidism in adult rat

Prolactin inhibition at the end of lactation programs for a central hypothyroidism in adult rat

Acclimation to different thermal conditions in a northerly wintering shorebird is driven by body mass-related changes in organ size

Mitochondrial glycerol 3-phosphate facilitates bumblebee pre-flight thermogenesis

Contact Info

Product

Resources

About