Direct and continuous measurements of water use by range grasses are needed by both range scientists and land managers. This study tested a heat balance sap flow gauge on individual culms of the tallgrass prairie species big bluestem (Andropogon gerurdii Vitman) and indiangrass [Sorghastrum nutuns (L.) Nash]. Gauge performance was evaluated on potted plants in the laboratory, greenhouse, and field by comparing sap flow to gravimetric measurements of transpiration. In the laboratory, gauge-measured water loss was consistently within 510% of gravimetric measurements for both species at flow rates 5 4 g hour'. The first-order time constant of the gauge was calculated to be ~20 seconds. In the greenhouse, sap flow estimates were consistently below gravimetric water loss and negative flows were often computed because of suspected errors in the radial heat flus component. Laboratory data showed that despite the gauge being surrounded with insulation, errors in the heat balance could occur because of external air temperature changes. In the field, environmental alterations in the stem energy balance affected the accuracy of gauges placed outside a plant canopy, but accurate measurements did occur when tbe plants were placed within a plant canopy. Heat transfer analysis indicated that foam insulation should be 20 to 25 mm thick to minimize the effect of the environment on gauge performance. water use by a rangeland shrub been measured directly and continuously under field conditions (Dugas and Mayeux 1991, Dugas et al. 1992). In general, the use of sap flow gauges has been almost entirely restricted to large dicots (Fichtner and Schulze 1990, Ham and Heilman 1991, Steinberg et al. 1991, Groot and King 1992). Far fewer studies have been reported using sap flow gauges on grass species, and these have been limited to larger agronomic species lie corn (Zea mays L.) (Gavloski et al. 1992). Only Sakuratani (1979, 1990) has reported sap flow measurements on a small cereal, rice (Oryza sutiva L.), but the gauge used was not removable and had to be built directly on the plant stem. No studies document the use of sap flow gauges on rangeland grass species. A removable sap flow gauge has been designed that can accommodate the small diameter and often irregular shaped stems of native grasses (Senock and Ham 1993). The objective of this study was to test and validate this new gauge design for use on prairie grasses and assess the utility or value of this gauge for use on rangelands.