Short-term effect of glucagon on gluconeogenesis and pyruvate kinase in rabbit hepatocytes

Zaleski, Jan; Zabłocki, Krzysztof; Bryła, Jadwiga

doi:10.1016/0020-711x(82)90010-6

Cited by 14 publications

(1 citation statement)

References 49 publications

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“…For measurements of glucose production the re actions were stopped at 60 min of incubation by the addition of 1 ml sample to 0.1 ml of 35% H C104. The metabolite content of kidney tubules was measured in samples taken at 60 min of incubation following centrif ugation through silicone oil into the perchloric acid solu tion as described previously [6].…”

Section: Methodsmentioning

confidence: 99%

Calcium-Dependent Effect of Gentamicin on Glucose Formation in Isolated Rabbit Kidney-Cortex Tubules

Michalik

Biedermann²,

Bryła³

1990

Kidney Blood Press Res

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In contrast to the inhibition by gentamicin of glucose production from propionate, pyruvate and lactate in renal tubules incubated at 2.5 mM Ca²⁺, this antibiotic does not affect gluconeogenesis from propionate and lactate, and significantly stimulates this process from other substrates at 0.5 mM Ca²⁺. This may be due to the gentamicin-induced increase of the cytosolic manganese content (from 1.7 to 2.7 nmol/mg protein), resulting in a stimulation of cytosolic phosphoenolpyruvate carboxykinase activity. At 2.5 mM Ca²⁺ the cytosolic Mn content (2.7 nmol/mg protein) seems to be high enough to accomplish activation of the enzyme.

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Section: Methodsmentioning

confidence: 99%