Short-term crocidolite inhalation studies in mice: validation of an inhalation chamber

Rihn, Bertrand; Kauffer, E.; Martin, Patrick; Coulais, Catherine; Villa, Maria Pia; Bottin, M.C.; Vigneron, J. C.; Edorh, AP; Martinet, Nadine

doi:10.1016/0300-483x(96)03325-2

Cited by 10 publications

(14 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The average fiber density was 1,875 ± 1,216 fibers/cm3 (n = 24) when we considered only the fibers > 5 ,um in length and < 3 ,um in diameter. The fiber size distribution was similar to that of a previous study (12). The proportion of fiber that met Stanton's criteria (16) did not vary over the experiment time and remained < 3% of the overall fibers; thus the crocidolite aerosol used in our study was mainly composed of short fibers ( Table 1).…”

Section: Resultssupporting

confidence: 82%

“…Rendall, National Center for Occupational Health, Johannesburg, South Africa) that replaced the original Union Internationale Contre le Cancer (Geneva, Switzerland) sample when it ran out. The fiber size distribution of the gift batch is extensively described in a previous study (12). The elementary analysis of this crocidolite sample was checked by emission spectroscopy (ICAP 61E; Thermo Jarrell Ash, Franklin, MA).…”

Section: Methodsmentioning

confidence: 99%

“…We used 0.8-pm mixed ester cellulose membranes to sample the fibers, which were counted by phase-contrast microscopy (12). We assessed the fiber size distribution of either the aerosol or the bulk crocidolite sample by transmission electron microscopy (IOOCX; JEOL Ltd., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The ashes were gently homogenized in ultrapure water by handshaking, then filtered over a carbon-covered Nucleopore membrane (25 mm diameter, 0.4 pm pore size). We did not choose ultrasonic treatment because we previously demonstrated that this treatment can change the fiber size distribution (12). The retained particles were covered with a second layer of carbon.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Inhaled crocidolite mutagenicity in lung DNA.

Rihn

Coulais

Kauffer

et al. 2000

Environ Health Perspect

View full text Add to dashboard Cite

We used transgenic mice carrying the laI reporter gene to study the mutagenesis potential of asbestos crocidolite. The animals were exposed by nose-ondy inhalation to an aerosol containg 5.75 mg/m3 crocidolite dust for 6 hr/day and 5 consecutive days. After 1, 4, and 12 weeks, we examined four end points: the cytology of bronchoalveolar lavage, the lung load of crocidolite, the hydrophobic DNA adducts, and the mutations in the lacIreporter gene. Twelve weeks after exposure, nearly 10% of the inhaled fibers remained in the lung (227 ± 103 ng/mg lung). There was evidence of a typical inflammatory response consisting of multinudeate macrophages at weeks 4 and 12, whereas immediately after the exposure, we observed numerous polymorphonudear neutrophils. The mutant frequency significatively increased during the fourth week after the exposure: 13.5 x 10-5 in the exposed group versus 6.9 x 10-5 in the control group. The induction factor, defined by the ratio of checked mutants of exposed mice to checke mutants of control mice, was 1.96. The mutation spectrum of control lung DNA and exposed lung DNA was similar, suggesting the possible involvement ofa DNA repair decrease in crocidolite-treated animals. We used the 32p-posdabeling method and did not detect any increase of either 5 mC or bulky adduct in treated mice. This is the first study that demonstrates asbestos mutagenicity in vivo after a nose-only inhalation.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Inhaled crocidolite mutagenicity in lung DNA.

Rihn

Coulais

Kauffer

et al. 2000

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…Camara (1967) used similar bottles with a side exit where animals were placed to inhale, while Carlini (1973) placed the animals inside these bottles to inhale. Since then, new models have emerged to evaluate the effect of inhalation of different types of materials (Rihn et al 1996, Matthew et al 2001. Recently, inhalation boxes were used, which contained pieces of cotton moistened with the studied material, and these were fixed at the top of the walls of the flask (Komyia et al 2006); alternatively, filter-paper discs were moistened with these materials and secured to the flask walls (Takemoto et al 2008, Ito and Ito 2011.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of an inhalation chamber for in vivo tests

Silva

Bizzo

Fernandes

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The bioavailability, toxicity, and therapeutic efficacy of a drug is directly related to its administration route. The pulmonary route can be accessed by inhalation after fumigation, vaporization or nebulization. Thus, pharmacological and toxicological evaluation accessed by an apparatus specifically designed and validated for this type of administration is extremely important. Based on pre-existing models, an inhalation chamber was developed. This presents a central structure with five animal holders. The nebulized air passes directly and continuously through these holders and subsequently to an outlet. Evaluation of its operation was performed using clove essential oil, a nebulizer, and a flow meter. The air within the chamber was collected by static headspace and analyzed by gas chromatography with a flame ionization detector. For this purpose, a 2.5 minutes chromatographic method was developed. The air flow in each of the five outputs was 0.92 liters per minute. During the first minute, the chamber became saturated with the nebulized material. Homogeneous and continuous operation of the chamber was observed without accumulation of volatile material inside it for 25 minutes. The inhalation chamber works satisfactorily for in vivo tests with medicines designed to be administrated by inhalation.

show abstract

Effects of various man-made mineral fibers on cell apoptosis and viability

et al. 2005

View full text Add to dashboard Cite

Evaluating the pathogenic potentials of man-made mineral fibers (MMMF) is an important task performed by the European Community. Noting that it has been proposed that the use of laboratory animals for scientific tests should be reduced or phased out, macrophages then become the cells of choice for conducting in vitro studies. We have evaluated the in vitro toxicity of six commercial stonewool fibers (A, B1, B2, C, D, and E) on U-937 cells. The physical interaction between U-937 cells and MMMF was observed using scanning electron microscopy, and the cytotoxicity was evaluated by studying cell viability using MTT assay and cell apoptosis with an ELISA detection kit. Scanning electron microscopy (SEM) analysis has shown that long fibers can be covered by several macrophages, and that a small fiber can be completely engulfed by one cell. With 50 microg/mL of MMMF, a decrease in cell viability appeared after seven days of incubation, whereas 200 microg/mL induced loss of viability and apoptosis after one day. Fiber D, comprising a high proportion of fibers >20 microm in length and a high concentration of MgO, induced the highest loss in viability and the highest rate of apoptosis compared to the other five fibers. Whether this toxic effect is related to either the physical characteristics of the fibers (such as length), or to the high concentration of magnesium is still to be determined. Because the results can be rapidly obtained, the proposed model is suitable for studying the toxicities of mineral components, even if the tested concentrations are far from the ones reached in the lung.

show abstract

Short-term crocidolite inhalation studies in mice: validation of an inhalation chamber

Cited by 10 publications

References 18 publications

Inhaled crocidolite mutagenicity in lung DNA.

Inhaled crocidolite mutagenicity in lung DNA.

Development and evaluation of an inhalation chamber for in vivo tests

Effects of various man-made mineral fibers on cell apoptosis and viability

Contact Info

Product

Resources

About