Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry

Hadjoudja, Souad; Vignoles, Chantal; Deluchat, Véronique; Lenain, Jean-François; Jeune, Anne‐Hélène Le; Baudu, Michel

doi:10.1016/j.aquatox.2009.07.007

Cited by 91 publications

(52 citation statements)

References 45 publications

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“…This is demonstrated in our results by the lower esterase activity values under high CO 2 compared to low CO 2 conditions, and lower values under high nutrient compared to low nutrient conditions. It is also important to note that the reduced esterase response for downregulated healthy cells differs from that observed in toxicological studies where a decrease in esterase activity is related to the impairment of cell metabolism due to acute damage (Hampel et al 2001, Hadjoudja et al 2009, Eigemann et al 2013. Low light conditions in our study were already within the range of saturating irradiances, while high light conditions were potentially photoinhibitory at midday since the LDPE cubitainers were UVR-transparent.…”

Section: Metabolic Activity and Cell Damagementioning

confidence: 87%

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Sobrino¹,

Segovia²,

Neale³

et al. 2014

Aquat. Biol.

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We studied the physiological response of phytoplankton to the interacting effects of 3 factors affected by global climate change: CO 2 , nutrient loading and irradiance. Treatments had a high and low level for each factor: CO 2 was bubbled at 1000 ppm by volume versus present atmo spheric values; high nutrient treatments had a combination of inorganic and organic nutrients; and light treatments were obtained by covering the tanks with a single or double layer of screen. We measured esterase activity, oxidative stress (ROS), cell death, DNA damage, photosynthetic efficiency and 14 C assimilation as particulate or dissolved organic material (POC and DOC respectively). Conditions simulating future global change scenarios showed similar chlorophyllnormalized primary productivity as present conditions. The main effect driving phytoplankton physiology was the downregulation of the photosynthetic apparatus by elevated CO 2 , which decreased esterase activity, ROS, cell death and DNA damage. Nutrient concentration and light acted as additional modulators, upregulating or contributing to downregulation. The percentage of DO 14 C extracellular release (PER) was low (0 to 27%), significantly lower under ultraviolet radiation (UVR) than under photosynthetically active radiation (PAR), and acted mainly to reequilibrate the internal balance when cells grown under UVR were exposed to PAR. PER was almost 3 times lower under high CO 2 , confirming a higher resource use efficiency of phytoplankton under future CO 2 concentrations.

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Section: Metabolic Activity and Cell Damagementioning

confidence: 87%

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Sobrino¹,

Segovia²,

Neale³

et al. 2014

Aquat. Biol.

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“…In particular, esterases comprise a key group of ubiquitous enzymes in plants and animals which have been shown to relate well to metabolic activity and cell viability (Dorsey et al 1989;Gala and Giesy 1990). Esterase activity has been used as a sensitive endpoint in different toxicity tests with microalgae, proving to be a reliable indicator of the toxic effects of pollutants (Hadjoudja et al 2009;Jamers et al 2009;Lage et al 2001;Yu et al 2007). A decline in enzyme activity is usually considered indicative of the presence of stress (Franklin et al 2001;Regel et al 2002).…”

Section: Discussionmentioning

confidence: 98%

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

et al. 2011

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Investigation of herbicide toxicology in non-target aquatic primary producers such as microalgae is of great importance from an ecological point of view. In order to study the toxicity of the widely used herbicide paraquat on freshwater green microalga Chlamydomonas moewusii, physiological changes associated with 96 h-exposures to this pollutant were monitored using flow cytometry (FCM) technique. Intracellular reactive oxygen species concentration, cytoplasmic membrane potential, metabolic activity and cell protein content were monitored to evaluate the toxicological impact of paraquat on algal physiology. Results showed that herbicide paraquat induced oxidative stress in C. moewusii cells, as it indicated the increase of both superoxide anion and hydrogen peroxide levels observed in non-chlorotic cells of cultures exposed to increasing herbicide concentrations. Furthermore, a progressive increase in the percentage of depolarised cells and a decrease in the metabolic activity level were observed in response to paraquat when non-chlorotic cells were analysed. Chlorotic cells were probably non-viable cells, based on the cytoplasmic membrane depolarisation, its metabolically non-active state and its drastically reduced protein content. In view of the obtained results, we have concluded that a range of significant physiological alterations, detected by flow cytometry, occur when C. moewusii, an ubiquitous microalga in freshwater environments, is challenged with environmentally relevant concentrations of the herbicide paraquat.

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“…The green fluorescence of phycobiont cells was examined using a flow cytometer (FCM, BD FACSAria, Becton Dickinson Co.). Excitation at 488 nm was induced by an argon laser and the emission was confined by a 515-545 nm interference filter [18]. BD FACSDiVa software (Becton Dickinson Co.) was used for instrument control and sample analysis.…”

Section: Experimental Design For Survival Analyses Of Symbiontsmentioning

confidence: 99%

Survival analyses of symbionts isolated from Endocarpon pusillum Hedwig to desiccation and starvation stress

Zhang

Wei

2011

Sci. China Life Sci.

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This work deals with the survival analyses of the symbionts isolated from the lichen E. pusillum under desiccation and starvation stress. The mycobiont of the symbionts was under the desiccation in combination with starvation stress, and under starvation stress alone as well. The phycobiont of the symbionts was under desiccation stress alone. The experiments were detected by means of the biomass size, weight and cell density, deformity of the hyphae and cells, and metabolic activity through SEM (scanning electron microscopy), TEM (transmission electron microscopy), FM (fluorescence microscopy), spectrophotometry, and FCM (flow cytometry). The results show that the mycobiont can survive for seven months under desiccation stress in combination with starvation stress, and for eight months under starvation stress alone. The phycobiont can survive for two months under desiccation stress. It can provide a scientific basis for further research of the reproduction biology of lichens and arid desert biocarpet engineering to fix sand and carbon. One of the central challenges of adversity-resistance biology is to understand the responses and adaptations of desert species to arid conditions. A lichen is a life-support system composed of a specific fungus (mycobiont) and a corresponding alga (phycobiont) or a cyanobacterium (cyanobiont) in an unique symbiotic relationship. "Their unique symbiotic relationship with algae has enabled these fungi to colonize and flourish in a wide range of habitats from the Antarctic continent to the rain forests of the tropics. Their ecological success in so many types of habitats depends on a number of unique structural and functional adaptations that are only now beginning to be generally appreciated by mycologists and microbiologists" [1]. Lichens are some of the most drought-resistant organisms; this characteristic has been extensively studied. In particular, levels of resistance to desiccation have been assessed using photosynthesis or respiration as indicators. Most of lichens are able to survive desiccation for several months [2−4], and a few species can survive for more than a year [4,5]. In reviewing previous studies, Lange [4] concluded that there was a general correlation between water conditions within the habitat and desiccation tolerance, i.e., the drier the habitat, the greater the desiccation tolerance of the lichen.Although the length of the desiccation period that lichens can survive is well documented, this information is still limited for isolated symbionts. Ahmadjian and Hale [6] reviewed the previous studies of desiccation resistance of isolated symbionts, and indicated that the isolated mycobionts could survive desiccation for five weeks (three species) or six weeks (seven species), while the isolated phycobionts could survive for six weeks (13 species) or 41 weeks (two

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Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry

Cited by 91 publications

References 45 publications

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Effect of CO2, nutrients and light on coastal plankton. IV. Physiological responses

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

Survival analyses of symbionts isolated from Endocarpon pusillum Hedwig to desiccation and starvation stress

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