Short-term changes of human acellular dermal matrix (Megaderm) in a mouse model

Abstract

BackgroundPhysicians tend to overcorrect when applying the acellular dermal matrix for reconstructive option because of volume decrement problem after absorption comparing with initial volume. However, there are no studies on the exact volume decrement and absorption rate with commercial products in South Korea. To figure out absorption rate of acellular dermal matrix product in South Korea (Megaderm), authors designed this experiment.MethodsNine mice were used and randomly divided into three groups by the time with sacrificing. The implant (Megaderm) was tailored to fit a cuboid form (1.0 cm× 1.0 cm in length and width and 2.0 mm in thickness). A skin incision was made at anterior chest with blade #15 scalpel with exposing the pectoralis major muscle. As hydrated Megaderm was located upon the pectoralis major muscle, the skin was sutured with Ethilon #5-0. After the surgical procedure, each animal group was sacrificed at 4, 8, and 12 weeks, respectively, for biopsies and histological analysis of the implants. All samples were stained with routine hematoxylin and eosin staining and Masson’s trichrome staining and the thickness were measured. A measurements were analyzed using Friedman test. Statistically, the correlation between thicknesses of Megaderm before and after implantation was analyzed.ResultsAfter sacrificing the animal groups at postoperative 4, 8, 12 weeks, the mean tissue thickness values were 2.10± 1.03 mm, 2.17± 0.21 mm, and 2.40± 0.20 mm (p= 0.368), respectively. The remaining ratios after absorption comparing with after initial hydrated Megaderm were 82.7%, 85.4%, and 94.5%, respectively. In histopathological findings, neovascularization and density of collagenous fiber was increased with time.ConclusionAuthor’s hypothesis was absorption rate of implant would be increased over time. But in this experiment, there is no statistical significance between mean absorption thickness of implant and the time (p= 0.368). Also it can be affected by graft site, blood supply, and animals that were used in the experiment.

Usefulness of Allogenic Acellular Dermal Matrix for Prevention of Scalp Depression after Burr Hole Trephination

“…Previous literatures has reported that implanted ADM is fully absorbed at a few weeks after graft implantation, and the thickness of the implanted ADM is inversely proportional with the absorption time via neovascularization, collagenous fiber formation, and the replacement of autologous tissue. 7 13) However, Kim et al 10) have reported no significant difference in absorption or thickness with time in a mouse model. Na et al 20) have shown the maintenance of ADM as a biological scaffold for a long period after implantation.…”

“…An allogenic acellular dermal matrix (ADM) is hypothesized to be an appropriate burr hole cover because of its use in other applications, including as a skin substitute, breast reconstructions, abdominal wall reconstruction, nasal augmentation in rhinoplasty, and orbital wall reconstruction. 8 9 10 14 17 18) However, to the best of our knowledge, there are no reports of ADM use in neurosurgery. In this study, we retrospectively assess the efficacy of ADM in patients following burr hole trephination since 2018.…”

Acellular dermis (SureDerm ® ) use for managing pulsatile tinnitus: a long-term follow-up of a novel surgical technique

“…They are composed of collagen and extracellular matrix components. The ideal ADMs undergo several technological processing steps involving decellularisation, disinfection, dehydration and sterilisation [ 13 ]. These steps allow easy transplantation without rejection and infection.…”

Cryptotia recurrence lowering technique with additional acellular dermal matrix graft

“…There were various studies on the ratio of ADM absorption. Kim et al [14] described no statistically significant decrease in ADM volume after 12 weeks of surgery in Murine model. Besides, other studies showed an absorption rate of 16% to 79.8% [15-18].…”

“…Besides, other studies showed an absorption rate of 16% to 79.8% [15-18]. Also, the absorption rate can vary sufficiently depending on graft site and blood supply [14]. This problem needs to be checked for a long-term result for cases that have been performed by an ADM graft in the posterior auricular sulcus.…”