Short-term changes in lymphocytes after placement of silver amalgam restorations in healthy subjects

Osorio, Estrella; Toledano, Manuel; Bravo, Manuel; Osorio, Raquel

doi:10.1016/0109-5641(95)80028-x

Cited by 8 publications

(2 citation statements)

References 9 publications

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“…The results of this study are consistent with those of Osorio et al They reported no alteration in the percentage of lymphocyte subsets before or after the placement of amalgam restorations 20 . Wilhelm et al were also unable to detect any changes in the total or relative counts of granulocytes, NK cells, B cells, cytotoxic T-cells and helper T-cells in patients with amalgam fillings 21 .…”

Section: Discussionsupporting

confidence: 83%

Relationship between percentage of regulatory T-cells and dental amalgam fillings.

et al. 2016

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Introduction: Regulatory T-cells are the main component of peripheral tolerance and their level is decreased in autoimmunity. In dental amalgam, a mixture of metals is used as a restorative material. During daily a ctivities, these metals are ingested and affect renal, neurosensory and immune systems. Studies have demonstrated an increased risk of autoimmune diseases in patients with dental amalgam fillings. It was hypothesized that the percentage of regulatory T-cells decreases in individuals with amalgam fillings. Therefore this study was designed to determine and compare the percentage of regulatory T-cells in individuals with and without amalgam fillings. Material and Methods: This was a cross-sectional study. Subjects were divided into two groups with each group consisting of 40 individuals. Group I (study group) comprised individuals with amalgam fillings, and Group II (control group), individuals without amalgam fillings in their teeth. Blood samples of all the participants were collected and tagged with CD4-FITC, CD25-PE and CD127-PerCP-Cy monoclonal antibodies for the detection of regulatory T-cells, FACSCalibur was used for this purpose. Results: The percentage of regulatory T-cells in the control group was high (77.77±5.54%) compared to the study group (76.09±7.68%), however, on comparison, the difference was not statistically significant (p=0.25). Conclusion: Dental amalgam fillings did not show a declining effect on the percentage of regulatory T-cells.

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Section: Discussionsupporting

confidence: 83%

Relationship between percentage of regulatory T-cells and dental amalgam fillings.

et al. 2016

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“…It is interesting to note that Henderson et al 50 also observed decreased lymphocyte responsiveness in some individuals receiving dental amalgam. Moreover, Osorio et al 51 observed transient effects of amalgam exposure on the distribution of subsets of T-cells.…”

Section: Discussionmentioning

confidence: 99%

Immune Function Effects of Dental Amalgam in Children

Shenker¹,

Maserejian²,

Zhang³

et al. 2008

The Journal of the American Dental Association

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Background Dental amalgam is a widely used restorative material containing 50% elemental mercury that emits mercury vapor. No randomized clinical trials have determined whether there are adverse immunologic effects associated with this low-level mercury exposure in children. The objective of this study was to evaluate a sub-population of the New England Children’s Amalgam Trial (NECAT) for in vitro manifestations of immunotoxic effects of dental amalgam. Methods A randomized clinical trial in which children requiring dental restorative treatment were randomized to either amalgam for posterior restorations or resin composite. A total of 66 children, aged 6–10 years, were assessed for total white cell numbers, T-cell, B-cell, neutrophil and monocyte responsiveness over a five-year period. Owing to the small number of participants, the study is exploratory in nature with limited statistical power. Results The mean number of tooth surfaces restored during the five-year period was 7.8 for the amalgam group and 10.1 for composite group. In the amalgam group there was a slight, but not statistically significant, decline in responsiveness of T-cells and monocytes at 5–7 days post treatment; no differences were consistently observed at 6, 12 or 60 months. Conclusions This study confirms that treatment of children with dental amalgams leads to increased, albeit low level, exposure to mercury. In this exploratory analysis of immune function, amalgam exposure did not cause overt immune deficits, although small transient effects were observed 5–7 days post restoration. Clinical implications These findings suggest that immunotoxic effects of amalgam restorations in children need not be a concern when choosing this restorative dental material.

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Mercury-Induced Apoptosis in Human Lymphoid Cells: Evidence That the Apoptotic Pathway Is Mercurial Species Dependent

Shenker

Guo

Shapiro

2000

Environmental Research

104

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Short-term changes in lymphocytes after placement of silver amalgam restorations in healthy subjects

Cited by 8 publications

References 9 publications

Relationship between percentage of regulatory T-cells and dental amalgam fillings.

Relationship between percentage of regulatory T-cells and dental amalgam fillings.

Immune Function Effects of Dental Amalgam in Children

Mercury-Induced Apoptosis in Human Lymphoid Cells: Evidence That the Apoptotic Pathway Is Mercurial Species Dependent

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