Short-term Cafeteria Diet Is Associated with Fat Mass Accumulation, Systemic and Amygdala Inflammation, and Anxiety-like Behavior in Adult Male Wistar Rats

Reis, Clarisse Giovana Maciel; Rocha-Gomes, Arthur; Teixeira, Amanda Escobar; Oliveira, Dalila Gomes de; Santiago, Camilla Mainy Oliveira; Silva, Alexandre A. da; Riul, Tania Regina; Oliveira, Eliza

doi:10.1016/j.neuroscience.2023.02.001

Cited by 7 publications

(12 citation statements)

References 76 publications

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“…Here, in the gestation period, the dams that received an HFD had a lower energy intake. The lower energy intake of these groups may be explained by the diet density, which provides greater satiety (dos Giovana Maciel Reis et al, 2023). However, this is an unexpected outcome that requires further investigation in the future.…”

Section: Discussionmentioning

confidence: 94%

“…The average weekly and cumulative energy intake were calculated. Similarly, the dams were evaluated during the gestation and lactation periods in relation to feeding and body weight (Giovana Maciel Reis et al, 2023).…”

Section: Nutritional Evaluationsmentioning

confidence: 99%

“…On PND21 (dams and offspring) and PND50 (remaining offspring), before euthanasia, the animals were anesthetized with ketamine (60 mg.kg −1 ) and xylazine (15 mg.kg −1 ) to undergo dual-energy X-ray absorptiometry (DEXA) and evaluation of the percentage of total fat mass. Body length was determined by the distance from the snout to the anus (Giovana Maciel Reis et al, 2023).…”

Section: Nutritional Evaluationsmentioning

confidence: 99%

“…Analyzes with the Labtest kits are carried out using an enzymatic-colorimetric method and readings using a spectrophotometer. Non-HDL levels were calculated (Giovana Maciel Reis et al, 2023).…”

Section: Nutritional Evaluationsmentioning

confidence: 99%

“…The whole hypothalamus was dissected according to the coordinates described in Paxinos and Watson (2014). The hypothalamus (PND21; PND50 offspring) and blood (dams) were used for the assessment of interleukin-6 (IL-6) and tumor necrosis factor-α (TNFα) using the enzyme-linked immunosorbent assay according to the manufacturer's instructions (R&D Systems;Giovana Maciel Reis et al, 2023).…”

Section: Cytokines Measurementmentioning

confidence: 99%

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Maternal high‐fat diet associated with LPS gestational injection induces hypothalamic inflammation and metabolic disorders in male Wistar rat offspring

Rocha‐Gomes,

Escobar Teixeira,

da Silva

et al. 2024

Developmental Psychobiology

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Maternal high‐fat diet (HFD) is linked to obesity and inflammation, predisposing offspring to metabolic and nutritional disorders. Accordingly, elevated blood levels of lipopolysaccharide (LPS) are also related to inflammation and metabolic complications in the offspring. The aim of this study was to evaluate the effects of the association of maternal HFD (gestation and lactation) and the LPS injection (gestation) on metabolic, inflammatory, and redox status parameters in male adolescent offspring. Female pregnant Wistar rats received randomly a standard or an HFD during gestation and lactation. On gestation Days 8, 10, and 12, half of the females in each group were intraperitonially injected with LPS (0.1 mg.kg−1). After weaning, all offspring received a standard diet. The dams and part of the male offspring were evaluated at weaning (Postnatal Day [PND] 21; food intake and inflammatory parameters), while the rest of the male offspring were evaluated during adolescence (PND50; food intake, redox status, and inflammatory parameters). HFD dams showed during gestation a lower weight gain. After lactation, HFD and LPS+HFD dams reported higher fat mass accumulation and increased interleukin‐6 (IL‐6) blood levels. HFD and LPS+HFD offspring showed at weaning higher levels of fat mass, body weight, and body length, besides an increased in hypothalamic IL‐6 levels. Noteworthy, at PND50, the LPS+HFD offspring showed higher energy intake, fat mass, and hypothalamic IL‐6 levels, in addition to an increased sucrose preference. Therefore, LPS+HFD offspring presented a worsening in energy metabolism, which was probably due to persistent hypothalamic inflammation, and also have a predisposition for the consumption of sweet foods.

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Section: Discussionmentioning

confidence: 94%

Section: Nutritional Evaluationsmentioning

confidence: 99%

Section: Nutritional Evaluationsmentioning

confidence: 99%

Section: Nutritional Evaluationsmentioning

confidence: 99%

Section: Cytokines Measurementmentioning

confidence: 99%

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