Short tandem repeat (STRs) and sex specific Amelogenin analysis of blood samples from neurosurgical female transfused patients

Mukherjee, Kanchan Kumar; Biswas, Ratna

doi:10.1016/j.jcfm.2004.06.003

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…5A), and identified subsequently from the uninfected sample as a conserved transcript showing identity with Mus musculus Sub1 mRNA (GenBank: NM011294) and with H. sapiens p15 cDNA encoding an activated RNA polymerase II transcription factor at chromosome locus 5p13.3 (GenBank: BC022339.1). Note that the stretch of LINE-1 mobilizing kDNA inserted in the p15 gene showed identity with H. sapiens, and that samples of U-937 macrophage line used in this study were re-authenticated by molecular tests (Lukka et al 2005, Mukherjee & Biswas 2005; mouse DNA template was used as a negative control in authentication (data not shown). Hybridization of RNA from threeyear-old G10 culture with a p15-specific probe confirmed its absence as a stable mRNA species (Fig.…”

Section: Mobilization Of the Minicircle Integration -mentioning

confidence: 90%

Hitchhiking Trypanosoma cruzi minicircle DNA affects gene expression in human host cells via LINE-1 retrotransposon

Simões-Barbosa

Argañaraz

Barros

et al. 2006

Mem. Inst. Oswaldo Cruz

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Full-length RNA transcribed from the human LINE-1 (Li) element Li Homo sapiens (LlHs) has a 900-nt, G+C-rich, 5'-untranslated region (UTR). The 5' UTR is followed by two long open reading frames, ORF1 and ORF2, which are separated from each other by an inter-ORF region of 33 nt that includes two or three in-frame stop codons. We examine here the mechanism(s) by which the translation of LlHs ORF1 and ORF2 is initiated. A stable hairpin structure (AG =-74.8 kcal/mol), inserted at nt 661 of the 5' UTR, caused a 3-to 8-fold decrease in the in vitro and in vivo translation ofeither a lacZ reporter gene for ORF1 or the ORF1 polypeptide product, p40, but translation of a lacZ reporter gene in ORF2 was increased. The results are compatible with a model for ORF1 translation initiation in which the majority of ribosomes scan from a point 5' of nt 661 but suggest that ORF2 is not translated by attached ribosomes that reinitiate after the termination of ORF1 translation. Our data are compatible with a model whereby the translation of LlHs ORF2 is initiated internally. The human LINE-1 (Li) element (Li Homo sapiens; LiHs) is the only known transposable element that is endogenous to the human genome. The structural features of LlHs (sche-matically presented in Fig.

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Section: Mobilization Of the Minicircle Integration -mentioning

confidence: 90%

Hitchhiking Trypanosoma cruzi minicircle DNA affects gene expression in human host cells via LINE-1 retrotransposon

Simões-Barbosa

Argañaraz

Barros

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…The insignificant amount of biological material to DNA extraction may result in absence of the target sequence in the fraction used for the reaction or the same can be degraded, not allowing DNA amplification by polymerase chain reaction (PCR). [9] Our study indicates that teeth could possibly show inconsistent results for DNA analysis after acid treatment for more than 5 h. The results, however, should be seen in perspective of different tooth types used in the study, age of teeth, and absence of jaw and soft tissue protection for the teeth.…”

Section: Discussionmentioning

confidence: 75%

Assessment of morphological changes and DNA quantification: An in vitro study on acid-immersed teeth

K¹,

Sudheendra²,

Khan³

et al. 2013

J Forensic Dent Sci

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Context:Acid immersion of victim's body is one of the methods employed to subvert identification of the victim, and hence of the perpetrator. Being hardest and chemically the most stable tissue in the body, teeth can be an important forensic investigative medium in both living and nonliving populations. Teeth are also good reservoirs of both cellular and mitochondrial DNA; however, the quality and quantity of DNA obtained varies according to the environment the tooth has been subjected to. DNA extraction from acid-treated teeth has seldom been reported.Aims:The objectives of the present study were to assess the morphological changes along with DNA recovery from acid-immersed teeth.Materials and Methods:Concentrated hydrochloric acid, nitric acid, and sulfuric acid were employed for tooth decalcification. DNA was extracted on an hourly basis using phenol–chloroform method. Quantification of extracted DNA was done using a spectrophotometer.Results:Results showed that hydrochloric acid had more destructive capacity compared to other acids.Conclusion:Sufficient quantity of DNA was obtainable till the first 2 hours of acid immersion and there was an inverse proportional relation between mean absorbance ratio and quantity of obtained DNA on an hourly basis.

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“…A review of the literature identifies that previous authors have shown that blood transfusion does not affect the DNA profiling of living or dead individuals from blood. A summary of the literature is presented in Table 1 [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Can post-mortem blood be used for DNA profiling after peri-mortem blood transfusion?

Graham

Tsokos²,

Rutty

2005

Int J Legal Med

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The question of whether blood transfusions can affect DNA profiling is still a contentious issue throughout the forensic community. It is hypothesised that donor leucocytes present in the administered blood will be detected upon examination of recipient blood. In order to resolve this issue, a selection of theoretical experiments were carried out to determine how much donor DNA must be present for its detection in blood components. Five casework examples of material collected from individuals after massive transfusion, including a case of whole organ transplantation, were also investigated. The results indicated that filtration processes used during blood production do not allow the passage of enough donor leucocytes for detection using current forensic profiling techniques. No evidence of secondary profile alleles were found in any case, indicating that peri-mortem blood transfusion does not affect DNA profiling.

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Short tandem repeat (STRs) and sex specific Amelogenin analysis of blood samples from neurosurgical female transfused patients

Cited by 4 publications

References 18 publications

Hitchhiking Trypanosoma cruzi minicircle DNA affects gene expression in human host cells via LINE-1 retrotransposon

Hitchhiking Trypanosoma cruzi minicircle DNA affects gene expression in human host cells via LINE-1 retrotransposon

Assessment of morphological changes and DNA quantification: An in vitro study on acid-immersed teeth

Can post-mortem blood be used for DNA profiling after peri-mortem blood transfusion?

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