Short tandem repeat (STR) locus HUMD8S306 in a large population sample from Germany

Abstract

Applied DNA typing in medico-legal investigations, in criminalistic practice, and in paternity cases often relies on high inclusion and exclusion probabilities. For that reason, the short autosomal tandem repeat locus D8D306 was validated for forensic use and incorporated into a nonoverlapping multiplex reaction with HUMDHFRP2 and HUMCD4: The allele frequencies of D8S306 in four different regions of Germany (n = 1220 alleles) were determined for use in a population database; the allele distributions did not significantly deviate from each other. The hererozygosity of D8S306 is 83%, expected exclusion chance in stain cases is 96% (paternity cases: 69%), the lowest amount of successfully amplified DNA was 30 pg. The alleles are in Hardy-Weinberg equilibrium.

Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

“…After careful consideration, the following 15 markers were taken from the literature: D1S1656 [11][12][13], D7S1517 [14], D8S306 [15,16], D8S639 [17,18], D9S304 [19], D10S2325 [12,20], D11S488 [18,21], D12S391 [22][23][24][25], D14S608 [2,26], D16S3253 [26], D17S976 [27], D18S1270 [26], D19S253 [28], D20S161 [29], and D21S1437 [26]. The chromosomal locations of these 15 loci, as well as those of STR markers used in commercially available PCR multiplexes (e.g., Powerplex 16 system), were determined by searching the May 2004 human genome assembly using BLAT [30] (http://www.genome.…”

Forensic Science

“…A study was performed to detect changes in absorbance and fluorescence of complexes of DNA-STR fragments with specific intercalating dyes (550). The STR locus B8S306 was studied in a German population (551). Gender Identification.…”

Validation study and population data of 15 “new” STR loci: A highly discriminating set for paternity and kinship analysis

“…In order to extend the set of loci we screened the literature for candidate STR markers regarding variability and compatibility for integration in a multiplex design. After careful consideration the following 15 markers were selected from the literature: D1S1656 [1], D7S1517 [2], D8S306 [3], D8S639 [4], D9S304 [5], D10S2325 [6], D11S488 [7], D12S391 [8], D14S608 [9], D16S3253 [9], D17S976 [10], D18S1270 [9], D19S253 [11], D20S161 [12], D21S1437 [9]. We performed a population study and evaluated these loci regarding potential linkage also with respect to commercially available loci (Powerplex 16 system).…”