Short tandem repeat (STR) instability in the oral mucosa of patients submitted to fixed orthodontic therapy: a limitation of STR profile quality for human identification

Díaz-Rojas, Arturo Rafael; Angeles-Estrada, Lucía; Vielma, Nadia Mabel Pérez; Sánchez-Monroy, Virginia

doi:10.1007/s12024-021-00451-4

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…The STR number is highly variable from person to person and at different loci [ 45 , 46 ]. The currently used kits enable the analysis of STR polymorphisms of non-coding autosomal DNA fragments and sex chromosomes [ 47 ].…”

Section: Analysis Of Genetic Materialsmentioning

confidence: 99%

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Żarczyńska,

Żarczyński,

Tomsia

2023

Genes

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The analysis of genetic material may be the only way to identify an unknown person or solve a criminal case. Often, the conditions in which the genetic material was found determine the choice of the analytical method. Hence, it is extremely important to understand the influence of various factors, both external and internal, on genetic material. The review presents information on DNA and RNA persistence, depending on the chemical and physical factors affecting the genetic material integrity. One of the factors taken into account is the time elapsing to genetic material recovery. Temperature can both preserve the genetic material or lead to its rapid degradation. Radiation, aquatic environments, and various types of chemical and physical factors also affect the genetic material quality. The substances used during the forensic process, i.e., for biological trace visualization or maceration, are also discussed. Proper analysis of genetic material degradation can help determine the post-mortem interval (PMI) or time since deposition (TsD), which may play a key role in criminal cases.

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Section: Analysis Of Genetic Materialsmentioning

confidence: 99%

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Żarczyńska,

Żarczyński,

Tomsia

2023

Genes

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Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances

Angeles-Estrada¹,

Pérez-Soto

Pérez-Vielma

et al. 2023

Clin Oral Invest

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Oxidative stress and genotoxicity in oral epithelial cells of subjects undergoing Fixed Orthodontic Appliances

Angeles-Estrada

Pérez-Soto

Pérez-Vielma

et al. 2023

Preprint

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Objectives The aim of this study was to analyze changes in oxidative stress (OS) and the genotoxic damage in oral epithelial cells caused by fixed orthodontic appliances. Materials and methods Samples of oral epithelial cells were obtained from fifty-one healthy voluntary subjects who had an indication for orthodontic treatment. The samples were obtained before and after 6- and 9-months starting treatment. OS was evaluated by quantitation of 8-hydroxy-2’deoxyguanosine (8-OHdG) and by relative gene expression of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT). DNA degradation and instability were evaluated by multiplex PCR and fragment analysis for human identification. Results Quantitation of 8-OHdG evidenced increase during treatment, although not statistically significant. SOD showed increase of 2.5 and 2.6-fold after 6 and 9 moths of treatment. CAT shows an increase of 3-fold, after six months of treatment, while after 9 months of treatment the level expression decreased to level similar to that before treatment. DNA degradation was found in 8% and 12% of DNA samples after 6 and 9 months of treatment,respectively, while DNA instability was detected in only 2% and 8 % of DNA samples after 6 and 9 months of treatment, respectively. Conclusions Results show light changes in OS and genotoxicity after fixed orthodontic appliances, but also suggesting a biological adaptation response to treatment after 6 months. Clinical relevance OS in buccal cavity represents risk to oral and systemic diseases. Implementation of antioxidant supplementation, use of thermoplastic materials, and time reduction during orthodontic therapy may reduce the risk.

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Short tandem repeat (STR) instability in the oral mucosa of patients submitted to fixed orthodontic therapy: a limitation of STR profile quality for human identification

Cited by 3 publications

References 33 publications

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Nucleic Acids Persistence—Benefits and Limitations in Forensic Genetics

Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances

Oxidative stress and genotoxicity in oral epithelial cells of subjects undergoing Fixed Orthodontic Appliances

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