Short tandem repeat profiling for the authentication of cancer stem‐like cells

Abstract

Colorectal and glioblastoma cancer stem‐like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross‐contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.

Elesclomol-induced increase of mitochondrial reactive oxygen species impairs glioblastoma stem-like cell survival and tumor growth

“…GSC lines were validated by Short Tandem Repeat (STR) DNA fingerprinting. Nine highly polymorphic STR loci plus amelogenin (Cell ID™ System, Promega Inc., Madison, WI, USA) were used [ 26 ]. All GSC profiles were challenged against public databases to confirm authenticity [ 26 ].…”

Chlorpromazine induces cytotoxic autophagy in glioblastoma cells via endoplasmic reticulum stress and unfolded protein response

“…Anchorage-dependent cell lines T98G, U-251 MG and U-87 MG were cultured as previously reported [ 30 ]. Anchorage-independent TS#1, TS#83 and TS#163 are patient-derived cell lines from surgical samples classified according to the WHO [ 31 ] cultured, as described [ 32 – 34 ] and defined as glioma stem cells growing as neurospheres [ 35 ]. The human hTERT-immortalized retinal pigment epithelial cell line hTERT RPE-1 (henceforth RPE-1) [ 36 ] was courtesy of Giulia Guarguaglini, CNR, Rome, Italy.…”

Effects of the Combined Treatment with a G-Quadruplex-Stabilizing Ligand and Photon Beams on Glioblastoma Stem-like Cells: A Magnetic Resonance Study

“…The in vivo tumorigenic potential of GSCs was assessed by intracranial cell injection in immunocompromised mice, where GSCs were capable of recapitulating the patient tumor in terms of antigen expression and histological tissue organization. GSC lines were validated by short tandem repeat (STR) DNA fingerprinting, as previously described [ 73 ]. Clones from GSCs were obtained by seeding single cells onto a 96-well plate.…”