Short pulses of antigen induce in vitro an antibody response to haptenic determinants

Bernstein, Alon; Globerson, Amiela

doi:10.1016/0008-8749(74)90109-9

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…We believe that previous studies using unfractionated cell populations have given misleading results. They suggest (6,7,11) that a simple brief encounter with antigen, initiating a single cycle of patching, capping, endocytosis, and catabolism of antigen ( 5), could efficiently trigger the whole cascade. However, in such studies, the carryover of antigen nonspecifically trapped in a bulky, wet cell pellet is impossible to eliminate.…”

Section: Discussionmentioning

confidence: 99%

Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.

Pike¹,

Nossal²

1976

The Journal of Experimental Medicine

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A system was established to assess the requirement for continuous presence of antigen in B-lymphocyte activation to antibody formation. Mouse spleen B lymphocytes, enriched for cells bearing anti-NIP (hapten 4-hydroxy-3-iodo-5-nitrophenylacetic acid) receptors, were pretreated briefly with NIP-POL (polymerized flagellin) antigen, washed, and added in small numbers to microcultures. The behaviour of these cells was compared with that of cells cultured in the continuous presence of antigen. Unfractionated spleen cells were studied under similar conditions. In contrast to unfractionated cells, enriched cells could not be triggered effectively by brief contact with antigen at any concentration tested. Fewer cells were activated, and clone size was smaller after brief contact with antigen than when antigen was present continuously. Furthermore, brief contact at high concentration did not cause tolerance induction.

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Section: Discussionmentioning

confidence: 99%

Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.

Pike¹,

Nossal²

1976

The Journal of Experimental Medicine

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“…We employed for this purpose an in vitro test model for an immune response [6], in which extracts of decidual tissue originating in pseudopregnant rats were applied t o cultures stimulated t o produce antibodies t o a-2,4dinitrophenyl-polylysine (DNP). …”

Section: Decidual Extracts Suppress Antibody Response In V I Mmentioning

confidence: 99%

Decidual extracts suppress antibody response in vitro

et al. 1977

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“…The organ culture technique enabling induction of an antibody response t o chemically defined synthetic antigens [8] was used according t o the modification of Bernstein and Globerson [9]. Antigen was applied directly on the spleen fragments, which were subsequently overlaid with culture medium (0.15 ml/culture).…”

Section: Culture Techniquementioning

confidence: 99%

“…We employed in vitro conditions [8,9] which enable a critical, controlled approach t o the examination of these questions. The results indicated that anti-hapten antibody response can be suppressed in vitro after its initiation in vivo by specific antigen, and in a more pronounced manner by the hapten coupled t o an unrelated carrier protein.…”

Section: Introductionmentioning

confidence: 99%

Suppression of anti‐hapten antibody response in vitro by hapten‐carrier conjugates

Bernstein

Globerson

1977

Eur J Immunol

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Production of antibodies was stimulated or suppressed arbitrarily by antigen treatment in vitro of spleens cultured at various time intervals after in vivo immunization. Spleens of mice immunized to the 2,4-dinitrophenyl or (4-hydroxy-3-iodo-5-nitrophenyl)acetyl haptenic determinants produced antibodies in culture when no antigen was applied in vitro. When a conjugate of the hapten to the same carrier employed for priming was given in vitro, an initial reduction of the response was observed, the level of which was dependent on antigen dose. Subsequently, increased amounts of antibodies were measured. In contrast, in vitro exposure to the hapten conjugated to an unrelated carrier resulted in significant reduction of the response for the entire period of the test. This suppressive effect manifested with various carrier proteins (ovalbumin, bovin IgG, bovine and rabbit serum albumin and keyhole limpet hemocyanin), when when applied to cultures in doses which were potentially immunogenic.

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Short pulses of antigen induce in vitro an antibody response to haptenic determinants

Cited by 10 publications

References 14 publications

Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.

Requirement for persistent extracellular antigen in cultures of antigen-binding B lymphocytes.

Decidual extracts suppress antibody response in vitro

Suppression of anti‐hapten antibody response in vitro by hapten‐carrier conjugates

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