Short peptides conjugated to non-peptidic motifs exhibit antibacterial activity

Ardila-Chantre, Natalia; Hernández-Cardona, Angie Katherine; Pineda-Castañeda, Héctor Manuel; Estupiñán-Torres, Sandra Mónica; Leal-Castro, Aura Lucía; Fierro-Medina, Ricardo; Rivera‐Monroy, Zuly Jenny; García‐Castañeda, Javier Eduardo

doi:10.1039/d0ra05937d

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…Therefore, the precursor of AC7 was obtained by combining the effective fragments from both Cecropin A and Abaecin. To enhance the α-helicity of the antimicrobial peptide, an α-helix sequence LLRR − was ligated to the C-terminal end of the primitive peptide of AC7. The incorporation of charged residues to disrupt hydrophobic surfaces has been found to significantly reduce hemolytic activity while maintaining antimicrobial efficacy .…”

Section: Discussionmentioning

confidence: 99%

Rational Design and Antimicrobial Potency Assessment of Abaecin Analogues

Yang,

Wu,

Weng

et al. 2023

ACS Biomater. Sci. Eng.

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Section: Discussionmentioning

confidence: 99%

Rational Design and Antimicrobial Potency Assessment of Abaecin Analogues

Yang,

Wu,

Weng

et al. 2023

ACS Biomater. Sci. Eng.

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“…It exhibits hydrophobic behavior and has a flexible structure and adequate space between its terminal amino and carboxyl groups that give this molecule the possibility of being used as a spacer and linker of fragments of active molecules. Ahx does not necessarily generate antimicrobial activity by itself, but when conjugated with peptides, it can significantly improve antimicrobial activity [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antifungal Activity of Chimeric Peptides Derived from Bovine Lactoferricin and Buforin II against Cryptococcus neoformans var. grubii

et al. 2022

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Cryptococcosis is associated with high rates of morbidity and mortality. The limited number of antifungal agents, their toxicity, and the difficulty of these molecules in crossing the blood–brain barrier have made the exploration of new therapeutic candidates against Cryptococcus neoformans a priority task. To optimize the antimicrobial functionality and improve the physicochemical properties of AMPs, chemical strategies include combinations of peptide fragments into one. This study aimed to evaluate the binding of the minimum activity motif of bovine lactoferricin (LfcinB) and buforin II (BFII) against C. neoformans var. grubii. The antifungal activity against these chimeras was evaluated against (i) the reference strain H99, (ii) three Colombian clinical strains, and (iii) eleven mutant strains, with the aim of evaluating the possible antifungal target. We found high activity against these strains, with a MIC between 6.25 and 12.5 µg/mL. Studies were carried out to evaluate the effect of the combination of fluconazole treatments, finding a synergistic effect. Finally, when fibroblast cells were treated with 12.5 µg/mL of the chimeras, a viability of more than 65% was found. The results obtained in this study identify these chimeras as potential antifungal molecules for future therapeutic applications against cryptococcosis.

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“…Additionally, mass spectrometry could be useful as a complementary technique to validate results based on computational methods for identifying new AMPs, 149–152 characterizing conjugated AMPs, 153 and evaluating susceptibility to proteases. 154…”

Section: Bioinformatics Analysis For Discovering New and Effective Am...mentioning

confidence: 99%