Short Oxygenated Warm Perfusion With Prostaglandin E1 Administration Before Cold Preservation as a Novel Resuscitation Method for Liver Grafts From Donors After Cardiac Death in a Rat In Vivo Model

Maida, Kai; Akamatsu, Yorihiro; Hara, Yasuyuki; Tokodai, Kazuaki; Miyagi, Shigehito; Kashiwadate, Toshiaki; Miyazawa, Kohtaro; Kawagishi, Naoki; Ohuchi, Noriaki

doi:10.1097/tp.0000000000001127

Cited by 24 publications

(8 citation statements)

References 20 publications

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“…Extra‐small partial liver transplantation was performed according to the cuff method for portal vein reconstruction with re‐arterialization 39,43‐45 . The 20% liver graft comprised the superior right lobe and inferior right lobe.…”

Section: Methodsmentioning

confidence: 99%

Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model

Shono

Kushida

Wakao

et al. 2021

American Journal of Transplantation

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Small‐for‐size syndrome (SFSS) has a poor prognosis due to excessive shear stress and sinusoidal microcirculatory disturbances in the acute phase after living‐donor liver transplantation (LDLT). Multilineage‐differentiating stress enduring (Muse) cells are reparative stem cells found in various tissues and currently under clinical trials. These cells selectively home to damaged sites via the sphingosine‐1‐phosphate (S1P)–S1P receptor 2 system and repair damaged tissue by pleiotropic effects, including tissue protection and damaged/apoptotic cell replacement by differentiating into tissue‐constituent cells. The effects of intravenously administered human bone marrow‐Muse cells and ‐mesenchymal stem cells (MSCs) (4 × 105) on liver sinusoidal endothelial cells (LSECs) were examined in a rat SFSS model without immunosuppression. Compared with MSCs, Muse cells intensively homed to the grafted liver, distributed to the sinusoids and vessels, and delivered improved blood chemistry and Ki‐67(+) proliferative hepatocytes and ‐LSECs within 3 days. Tissue clearing and three‐dimensional imaging by multiphoton laser confocal microscopy revealed maintenance of the sinusoid continuity, organization, and surface area, as well as decreased sinusoid interruption in the Muse group. Small‐interfering RNA‐induced knockdown of hepatocyte growth factor and vascular endothelial growth factor‐A impaired the protective effect of Muse cells on LSECs. Intravenous injection of Muse cells might be a feasible approach for LDLT with less recipient burden.

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Section: Methodsmentioning

confidence: 99%

Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model

Shono

Kushida

Wakao

et al. 2021

American Journal of Transplantation

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“…In liver, studies on therapeutic medications during NMP to reduce IRI showed promising results in pigs and rats [133][134][135] . Goldaracena et al delivered an antiviral drug to perfusate during normothermic ex vivo liver perfusion and effectively induced Hepatitis C virus resistance after pig liver transplantation [136] .…”

Section: Organ Repairmentioning

confidence: 99%

Organ preservation: from the past to the future

et al. 2018

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Organ transplantation is the most effective therapy for patients with end-stage disease. Preservation solutions and techniques are crucial for donor organ quality, which is directly related to morbidity and survival after transplantation. Currently, static cold storage (SCS) is the standard method for organ preservation. However, preservation time with SCS is limited as prolonged cold storage increases the risk of early graft dysfunction that contributes to chronic complications. Furthermore, the growing demand for the use of marginal donor organs requires methods for organ assessment and repair. Machine perfusion has resurfaced and dominates current research on organ preservation. It is credited to its dynamic nature and physiological-like environment. The development of more sophisticated machine perfusion techniques and better perfusates may lead to organ repair/reconditioning. This review describes the history of organ preservation, summarizes the progresses that has been made to date, and discusses future directions for organ preservation.

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“…It was suggested that subnormothermic temperatures may bridge the gap between metabolic retardation during cold hypothermia and hypoxia at 'hotter' normal physiological temperatures, enabling the maintenance of an approximate 25% of physiological metabolic levels in the liver graft [37]. In a number of animal and preclinical studies [18,[27][28][29][30]38,39] assessing the effects on resynthesis of tissue ATP by SNMP on livers of different extended criteria, DBD or DCD and steatosis were identified (Tables 2-4). Additionally, there are studies of a relatively new perfusion technique, controlled oxygenated rewarming (COR) developed by Minor et al [18] in Essen, Germany.…”

Section: Positive Effects Of Subnormothermic Perfusion Temperatures (mentioning

confidence: 99%

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

Bellini

Yiu

Nozdrin

et al. 2019

JCM

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The recent advances in machine perfusion (MP) technology involve settings ranging between hypothermic, subnormothermic, and normothermic temperatures. Tissue level adenosine triphosphate (ATP) is a long-established marker of viability and functionality and is universal for all organs. In the midst of a growing number of complex clinical parameters for the quality assessment of graft prior to transplantation, a revisit of ATP may shed light on the underlying reconditioning mechanisms of different perfusion temperatures in the form of restoration of metabolic and energy status. This article aims to review and critically analyse animal and preclinical human studies (discarded grafts) during MP of three abdominal organs (liver, kidney, and pancreas) in which ATP was a primary endpoint. A selective review of recent novel reconditioning approaches relevant to mitigation of graft ischaemia-reperfusion injury via MP and for different perfusion temperatures was also conducted. With a current reiterated interest for oxygenation during MP, a re-introduction of tissue ATP levels may be valuable for graft viability assessment prior to transplantation. Further studies may help delineate the benefits of selective perfusion temperatures on organs viability.

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Short Oxygenated Warm Perfusion With Prostaglandin E1 Administration Before Cold Preservation as a Novel Resuscitation Method for Liver Grafts From Donors After Cardiac Death in a Rat In Vivo Model

Abstract: Short oxygenated warm perfusion before cold preservation and the addition of PGE1 to SOWP were thus beneficial in an in vivo rat model.

Cited by 24 publications

References 20 publications

Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model

Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model

Organ preservation: from the past to the future

The Effect of Preservation Temperature on Liver, Kidney, and Pancreas Tissue ATP in Animal and Preclinical Human Models

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