Short Neuropeptide F Acts as a Functional Neuromodulator for Olfactory Memory in Kenyon Cells of<i>Drosophila</i>Mushroom Bodies

Knapek, Stephan; Kahsai, Lily; Winther, Åsa M.E.; Tanimoto, Hiromu; Nässel, Dick R.

doi:10.1523/jneurosci.2287-12.2013

Cited by 46 publications

(41 citation statements)

References 58 publications

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“…The influence of sNPF on feeding behavior and growth is well described [109][110][111], but numerous other roles have been reported [86,112]. We detected short NPF-2 (SPSLRLRFa), one of the four predicted sNPF neuropeptides, in the brain, TG, and CC-CA.…”

Section: Short Neuropeptide Fmentioning

confidence: 62%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

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Section: Short Neuropeptide Fmentioning

confidence: 62%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…Alternatively, it may simply reflect a difference in their frequency of utilization in different circuits. Whatever the case, both dNPF and sNPF have been implicated in additional functions besides hunger modulation (Kahsai et al, 2010; Kapan et al, 2012; Kim et al, 2013; Knapek et al, 2013; Lee et al, 2006; Shohat-Ophir et al, 2012), indicating that their effects on behavior are context-dependent.…”

Section: Discussionmentioning

confidence: 99%

Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

Inagaki

Panse

Anderson

2014

Neuron

160

224

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SUMMARY An organism’s behavioral decisions often depend upon the relative strength of appetitive and aversive sensory stimuli, the relative sensitivity to which can be modified by internal states like hunger. However, whether sensitivity to such opposing influences is modulated in a unidirectional or bidirectional manner is not clear. Starved flies exhibit increased sugar and decreased bitter sensitivity. It is widely believed that only sugar sensitivity changes, and that this masks bitter sensitivity. Here we use gene- and circuit-level manipulations to show that sweet- and bitter-sensitivity are independently and reciprocally regulated by starvation in Drosophila. We identify orthogonal neuromodulatory cascades that oppositely control peripheral taste sensitivity for each modality. Moreover, these pathways are recruited at increasing hunger levels, such that low-risk changes (higher sugar sensitivity) precede high-risk changes (lower sensitivity to potentially toxic resources). In this way, state intensity-dependent, reciprocal regulation of appetitive and aversive peripheral gustatory sensitivity permits flexible, adaptive feeding-decisions.

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“…It is possible that compensatory changes in the prt mutant mitigate its effect on learning. Alternatively, the release of a peptide, rather than a classical neurotransmitter, may be more important for some functions of KCs (Iliadi et al, 2008; Knapek et al, 2013). …”

Section: Drosophila Vesicular Neurotransmitter Transportersmentioning

confidence: 99%

Drosophila melanogaster as a genetic model system to study neurotransmitter transporters

Martin¹,

Krantz

2014

Neurochemistry International

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The model genetic organism Drosophila melanogaster, commonly known as the fruit fly, uses many of the same neurotransmitters as mammals and very similar mechanisms of neurotransmitter storage, release and recycling. This system offers a variety of powerful molecular-genetic methods for the study of transporters, many of which would be difficult in mammalian models. We review here progress made using Drosophila to understand the function and regulation of neurotransmitter transporters and discuss future directions for its use.

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Short Neuropeptide F Acts as a Functional Neuromodulator for Olfactory Memory in Kenyon Cells ofDrosophilaMushroom Bodies

Cited by 46 publications

References 58 publications

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Independent, Reciprocal Neuromodulatory Control of Sweet and Bitter Taste Sensitivity during Starvation in Drosophila

Drosophila melanogaster as a genetic model system to study neurotransmitter transporters

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