Short Hairpin RNA (shRNA) Ether à go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling

Wu, Jin; Wu, Xinyu; Zhong, Donglai; Zhai, Wenliang; Ding, Zhenqi; Zhou, Yong

doi:10.3390/ijms131012573

Cited by 34 publications

(31 citation statements)

References 26 publications

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“…A number of studies reported that VM existed in various tumors including ovarian cancer [5,6,7,8]. There is an intimate link between VM and tumor and this connection could enhance tumor growth and metastasis by providing an adequate vascular supply [9].…”

Section: Introductionmentioning

confidence: 99%

Association of Vasculogenic Mimicry Formation and CD133 Expression with Poor Prognosis in Ovarian Cancer

Liang

Yang²,

Cao³

et al. 2016

Gynecol Obstet Invest

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Aims: This study was conducted to investigate the association of vasculogenic mimicry (VM) formation and CD133 expression with the clinical outcomes of patients with ovarian cancer. Methods: This retrospective study was performed in 120 ovarian carcinoma samples. VM formation and CD133 expression was identified with CD31/periodic acid-Schiff double-staining and CD133 immunohistochemical staining. Collected clinical and pathological data included age at diagnosis, histologic type, tumor grade, tumor stage, lymph node metastases and response to chemotherapy. The overall survival time was calculated. Results: VM was identified in 52 (43%) of 120 ovarian carcinoma tissues and CD133 expression was found in 56 (47%) cases. Both VM formation and CD133 expression were associated with advanced tumor stage, high-grade carcinoma and non-response to chemotherapy (p < 0.05). They were also associated with shorter overall survival time (p < 0.05) by log-rank test. Combined marker of VM formation and CD133 expression was associated with high-grade ovarian carcinoma, late-stage disease, non-response to chemotherapy and shorter overall survival time (p < 0.05). Conclusions: VM formation and CD133 expression can provide additional prognostic information for patients with ovarian cancer. Combined marker of VM formation and CD133 expression may be a potent predictor for poor prognosis for patients with ovarian cancer.

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Section: Introductionmentioning

confidence: 99%

Association of Vasculogenic Mimicry Formation and CD133 Expression with Poor Prognosis in Ovarian Cancer

Liang

Yang²,

Cao³

et al. 2016

Gynecol Obstet Invest

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“…Zhu et al found that increased expression of miR-126 enhanced the sensitivity of NSCLC cells to chemotherapy agents through negative regulation of the VEGF/PI3K/Akt/MRP1 signaling pathway [32]. Results from another study suggest that Eag1 siRNA could inhibit tumor growth and angiogenesis in osteosarcoma through modulating the activation of VEGF/PI3K/AKT signaling [33]. Similar to their results, we found the protein levels of VEGFA were up-regulated in osteosarcoma tissues and in the osteosarcoma cell line SAOS-2 compared with adjacent nontumorous osteosarcoma tissues and normal human osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

The Down-Regulation of MicroRNA-497 Contributes to Cell Growth and Cisplatin Resistance Through PI3K/Akt Pathway in Osteosarcoma

Shao

Miao

Xue

et al. 2015

Cell Physiol Biochem

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Background: Down-expression of microRNA-497 (miR-497) was often found in malignancies. The purposes of this study were to determine the expression of miR-497 in human osteosarcoma and to establish the association between miR-497 expression with cell survival and the sensitivity to cisplatin in human osteosarcoma cells. Methods: The effects of ectopic miR-497 expression on the cell survival and cisplatin sensitivity in osteosarcoma cells were measured by the Cell Counting Kit-8 (CCK-8) assay. Quantitative real-time PCR (qRT-PCR) was utilized to determine the expression of miR-497. The effects of ectopic miR-497 expression on the expression of VEGFA, Akt and p-Akt were determined by western blot. Results: Real-time quantitative PCR analysis revealed that miR-497 was significantly down-regulated in osteosarcoma tissues and in the osteosarcoma cell line SAOS-2 compared with adjacent nontumorous osteosarcoma tissues and normal human osteoblasts. Up-regulation of miR-497 inhibited cell survival and enhanced the sensitivity to cisplatin in osteosarcoma cells. In addition, knockdown of miR-497 induced osteosarcoma cells growth and cisplatin resistance. Luciferase reporter assay and western blot confirmed that VEGFA was a direct target of miR-497. PI3K inhibitor LY294002 abrogated miR-497 inhibitors induced cisplatin resistance. Conclusion: Taken together, our results suggest that miR-497 modulates the sensitivity to cisplatin at least in part through PI3K/Akt pathway in osteosarcoma cells.

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“…Accordingly, several meta-analysis have shown that increased VEGF levels in patients are directly related to decreased survival and to tumour progression and consequently, to a poor prognosis [38,39,40] . Studies that have investigated molecular mechanisms involving VEGF showed the importance of regulating the VEGF/PI3K/AKT signalling pathway in the progression and development of osteosarcoma [41,42] .…”

Section: Vascular Endothelial Growth Factor (Vegf)mentioning

confidence: 99%

Emerging Cytokine Networks in Osteosarcoma

Lopes-Júnior

Silveira

Vulczak

et al. 2017

Can Cell Microenviron

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Osteosarcoma is the most prevalent primary bone cancer. Although it has a global impact of approximately 1 to 3 cases/million a year, its etiopathophysiology is still unknown. Moreover, the immune response to its development is very individual as well as variable. Particular emphasis has been placed on the study of the immunoregulatory role of cytokines in osteosarcoma. In this scenario, newly identified cytokine networks have emerged. A deep understanding on the complex interplay of cytokines in osteosarcoma may have prognostic importance. In this review, we seek to highlight the classic roles of cytokines in osteosarcoma, to describe the role of new players in the emerging cytokine networks, and to discuss the therapeutic targets that encompass the complexity and implications of this network.

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Short Hairpin RNA (shRNA) Ether à go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling

Cited by 34 publications

References 26 publications

Association of Vasculogenic Mimicry Formation and CD133 Expression with Poor Prognosis in Ovarian Cancer

Association of Vasculogenic Mimicry Formation and CD133 Expression with Poor Prognosis in Ovarian Cancer

The Down-Regulation of MicroRNA-497 Contributes to Cell Growth and Cisplatin Resistance Through PI3K/Akt Pathway in Osteosarcoma

Emerging Cytokine Networks in Osteosarcoma

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