Short day-photoperiod triggers and low temperatures increase expression of peroxidase RNA transcripts and basic peroxidase isoenzyme activity in grapevine buds

Noriega, Ximena; Burgos, Bárbara; Pérez, Francisco J.

doi:10.1016/j.phytochem.2007.02.003

Cited by 31 publications

(11 citation statements)

References 24 publications

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“…The second possibility seems more likely, since transcript levels of APX gene are upregulated following HC application to grapevine buds (Halaly et al 2008). Moreover, enhanced transcription was reported for APX, glutathione peroxidase (Pacey-Miller et al 2003) and POD (Noriega et al 2007) preceding the release of endodormancy in grapevine buds in response to chilling, natural inducer of budbreak. Thus, the observed increase in APX and POD activities in the present study may be due to the increase in the enzymes' levels.…”

Section: Discussionmentioning

confidence: 97%

Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds

Mohamed

Vadel

Geuns³

et al. 2011

Acta Physiol Plant

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Section: Discussionmentioning

confidence: 97%

Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds

Mohamed

Vadel

Geuns³

et al. 2011

Acta Physiol Plant

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“…Total RNA was isolated and purified from grapevine buds (0.5–0.7 g freshweight) using a modification of the method of Chang et al (1993), described in Noriega et al (2007). DNA was removed by treatment with RNAase-free DNAase (1 U/μg) (Invitrogen) at 37 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Hypoxia induces H2O2 production and activates antioxidant defence system in grapevine buds through mediation of H2O2 and ethylene

Vergara

Parada

Rubio

et al. 2012

Journal of Experimental Botany

108

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Paradoxically, in eukaryotic cells, hydrogen peroxide (H2O2) accumulates in response to oxygen deprivation (hypoxia). The source of H2O2 under hypoxia varies according to the species, organs, and tissue. In non-photosynthetic tissues, H2O2 is mainly produced by activation of NAD(P)H-oxidases or by disruption of the mitochondrial electron transport chain (m-ETC). This study showed that hypoxia, and inhibitors of respiration like potassium cyanide (KCN) and sodium nitroprusside (SNP), trigger the production of H2O2 in grapevine buds. However, diphenyleneiodonium, an inhibitor of NAD(P)H-oxidase, did not reduce the H2O2 levels induced by KCN, suggesting that, under respiratory stress, H2O2 is mainly produced by disruption of the m-ETC. On the other hand, γ-aminobutyric acid (GABA), a metabolite that in plants alleviates oxidative stress by activating antioxidant enzymes, reduced significantly the levels of H2O2 induced by KCN and, surprisingly, repressed the expression of genes encoding antioxidant enzymes such as ASCORBATE PEROXIDASE (VvAPX), GLUTATHIONE PEROXIDASE (VvGLPX), SUPEROXIDE DISMUTASE (VvSOD), and one of the CATALASE isoforms (VvCAT1), while VvCAT2 was upregulated. In contrast to GABA, hypoxia, H2O2, and ethylene increased dramatically the expression of genes encoding antioxidant enzymes and enzymes of the alternative respiratory pathway such as ALTERNATIVE NADH-DEHYDROGENASES (VvaNDs) and ALTERNATIVE OXIDASES (VvAOXs). Hence, it is concluded that H2O2 production is stimulated by respiratory stress in grapevine buds, that H2O2 and ethylene act as signalling molecules and activate genes related to the antioxidant defence system, and finally that GABA reduces H2O2 levels by up-regulating the expression of VvCAT2.

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“…15 buds.) using a modification of Chang et al (1993) method as described in Noriega et al (2007). DNA was removed by treatment with RNAase-free DNAase (1 U/lg) at 37°C for 30 min.…”

Section: Rna Extraction and Purificationmentioning

confidence: 99%

On the mechanism of dormancy release in grapevine buds: a comparative study between hydrogen cyanamide and sodium azide

Pérez

Vergara

2009

Plant Growth Regul

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Sodium azide (NaN 3 ), a well-known inhibitor of mitochondrial respiration, stimulated bud-dormancy release in grapevines similar to hydrogen cyanamide (HC), while HC, a well-known dormancy release agent, inhibited the O 2 uptake in isolated grape bud mitochondria similarly to NaN 3 . Additionally, both chemicals induced transcript expression of the antioxidative enzyme glutathione reductase and glucose-6-phosphate-dehydrogenase (G6PD), therefore upregulated the ascorbate-glutathione cycle (AGC) and the pentose phosphate pathway, respectively. As a result of AGC activation, the ratio of reduced to oxidized glutathione (GSH/GSSG) increased. Both stimuli also upregulated the transcription of 1,3-b-D-glucanase, a key enzyme in dormancy release. Together, these data support mechanistic connection between impaired Mit function and dormancy release, and suggests that as a consequence of O 2 deprivation, increases in glycolysis and in ethanolic fermentation could be responsible for activation of downstream stages in the dormancy release mechanisms.

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Short day-photoperiod triggers and low temperatures increase expression of peroxidase RNA transcripts and basic peroxidase isoenzyme activity in grapevine buds

Cited by 31 publications

References 24 publications

Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds

Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds

Hypoxia induces H2O2 production and activates antioxidant defence system in grapevine buds through mediation of H2O2 and ethylene

On the mechanism of dormancy release in grapevine buds: a comparative study between hydrogen cyanamide and sodium azide

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