Short communication: Virological and B cell profiles of chikungunya and Dengue virus co-infections in Delhi during 2017–2019

Ibemgbo, Sylvester; Nyodu, Rajni; Chaudhary, Sakshi; Verma, Dileep Kumar; Dixit, Kritika; Nayak, Kaustuv; Rani, Vandana; Gaind, Rajni; Chandele, Anmol; Sunil, Sujatha

doi:10.1016/j.virusres.2022.198888

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…A total of 130 human serum samples were used in the present study after obtaining informed consent from the patients in both India and Burkina Faso. Among the 130 serum samples, 32 were positive for DENV ( n = 16), and CHIKV ( n = 16) and 32 from uninfected individuals (negative for both viruses) were part of a study funded by the government of India [ 29 ]. Also, 33 of the 130 serum samples were positive only for DENV, and 33 serum samples were negative for DENV, kindly offered by the National Institute of Public Health, Burkina Faso ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Also, 33 of the 130 serum samples were positive only for DENV, and 33 serum samples were negative for DENV, kindly offered by the National Institute of Public Health, Burkina Faso ( Figure 1 ). Sera samples that were positive for the respective viruses by real-time RT PCR were selected for the study [ 29 ]. The protocol of this study was reviewed and approved by the ethical committees of the institutional ethical committees of the Health Science Research, Burkina Faso No.…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have previously reported distinct molecular assays developed using TaqMan real-time RT-PCR techniques or SYBR green base with universal DENV primers and RT-PCR with other reverse transcriptase for detecting dengue serotypes as well as other flaviviruses [11,24,25]. Similarly, commercial rapid diagnostic tests (RDTs) such as SD BIOLINE Chikungunya IgM, RTK ProDetect TM , and SD BIOLINE Dengue Duo ® are available for detecting both CHIKV and DENV serotypes [28,29]. However, reports of false-positive results have dampened the enthusiasm for the use of these kits by clinicians.…”

Section: Primer Design and Their Specificity Assessment For Multiplex...mentioning

confidence: 99%

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Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance

Belem,

Ibemgbo,

Gomgnimbou

et al. 2024

CIMB

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The major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPCR) were developed using reference strains of the CHIKV and DENV serotypes. The specificity of the test for all the viruses was confirmed through sequencing. The standard curves showed a high correlation coefficient, R2 = 0.99, for DENV-2 and DENV-3; R2 = 0.98, for DENV-4; and CHIKV; R2 = 0.93, for DENV-1. The limits of detection were calculated to be 4.1 × 10−1 copies/reaction for DENV-1, DENV-3, and CHIKV and 4.1 × 101 for DENV-2 and DENV-4. The specificity and sensitivity of the newly developed mRT-PCR and mRT-qPCR were validated using positive serum samples collected from India and Burkina Faso. The sensitivity of mRT-PCR and mRT-qPCR are 91%, and 100%, respectively. The specificity of both assays was 100%. mRT-PCR and mRT-qPCR assays are low-cost, and a combination of both will be a useful tool for arbovirus surveillance.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Primer Design and Their Specificity Assessment For Multiplex...mentioning

confidence: 99%

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Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance

Belem,

Ibemgbo,

Gomgnimbou

et al. 2024

CIMB

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Identification and validation of ferroptosis-related genes in patients infected with dengue virus: implication in the pathogenesis of DENV

Yan

et al. 2023

Virus Genes

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Ferroptosis, an iron-dependent form of regulated cell death, has been associated with many virus infections. However, the role of ferroptosis in dengue virus (DENV) infection remains to be clarified. In our study, a dengue fever microarray dataset (GSE51808) of whole blood samples was downloaded from the Gene Expression Omnibus (GEO), and a list of ferroptosis related genes (FRGs) was extracted from the FerrDb. We identified 37 ferroptosis-related differentially expressed genes (FR-DEGs) in DENV-infected patient blood samples compared to healthy individuals. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses as well as protein–protein interaction (PPI) network of FR-DEGs revealed that these 37 FR-DEGs were mainly related to the C-type lectin receptor and p53 signaling pathway. Nine out of the 37 FR-DEGs (HSPA5, CAV1, HRAS, PTGS2, JUN, IL6, ATF3, XBP1, and CDKN2A) were hub genes, of which 5 were validated by qRT-PCR in DENV-infected HepG2 cells. Finally, using miRNA-mRNA regulatory network, we identified has-miR-124-3p and has-miR-16-5p as the most critical miRNAs in regulating the expression of these hub genes. In conclusion, our findings demonstrated that 5 FR-DEGs, JUN, IL6, ATF3, XBP1, and CDKN2A, and two miRNAs, has-miR-124-3p and has-miR-16-5p may implicate an essential role of ferroptosis in DENV infection, and further studies are warranted to explore the underlying mechanisms. Supplementary Information The online version contains supplementary material available at 10.1007/s11262-023-01985-1.

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An overview of anti-Chikungunya antibody response in natural infection and vaccine-mediated immunity, including anti-CHIKV vaccine candidates and monoclonal antibodies targeting diverse epitopes on the viral envelope

Chandley,

Lukose,

Kumar

et al. 2023

The Microbe

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Short communication: Virological and B cell profiles of chikungunya and Dengue virus co-infections in Delhi during 2017–2019

Cited by 4 publications

References 19 publications

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance

Identification and validation of ferroptosis-related genes in patients infected with dengue virus: implication in the pathogenesis of DENV

An overview of anti-Chikungunya antibody response in natural infection and vaccine-mediated immunity, including anti-CHIKV vaccine candidates and monoclonal antibodies targeting diverse epitopes on the viral envelope

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