Short communication: Uterine leptospiral infection is strongly associated to strains of serogroup Sejroe on experimentally infected hamsters

Pinto, Priscila S.; Barbosa, Cristina; Ferreira, Ana Maria Reis; Lilenbaum, Walter

doi:10.1016/j.micpath.2020.104030

Cited by 8 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, there was no significant difference between vaccinated and unvaccinated sheep in urine PCR. Although renal colonization disappeared after four months (for all groups), this was most likely due to the intrinsic characteristics of the infecting strain (Guaricura FV52), since studies conducted on hamsters [ 13 ] and sheep [ 14 ] had demonstrated the tendency of an auto-limited renal infection of that strain. Indeed, the pathogenesis of that strain herein observed was very similar to that observed by Rocha et al [ 14 ].…”

Section: Discussionmentioning

confidence: 97%

“…After this, the isolate was transferred only once in EMJH medium for inoculum definition (10 8 leptospires/mL). This strain was chosen because it has already demonstrated its ability to colonize the uteri of hamsters [ 13 ] and sheep [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…In Brazil, Leptospira santarosai (strains of the serovar Guaricura, also from serogroup Sejroe) seem to be endemic in ruminants [ 11 ]. Besides having recovered from cervicovaginal mucus [ 5 ] and uteri [ 12 ] of asymptomatic naturally infected cows, the ability of these strains to colonize the uteri of experimentally infected hamsters [ 13 ] as well as sheep [ 14 ] has been demonstrated. Sheep have been described as a good experimental model to leptospirosis studies [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Efficacy of Vaccination in the Prevention of Renal and Genital Leptospirosis in Experimentally Infected Sheep

et al. 2022

View full text Add to dashboard Cite

(1) Background: Leptospirosis, mainly determined by strains belonging to serogroup Sejroe, has a direct impact on the reproductive efficiency of ruminants, such as sheep. In Brazil, Leptospira santarosai serovar Guaricura, known to be able to chronically colonize the uterine environment, is of special note. Although vaccination minimizes the effects of acute disease, whether or not it can protect from renal colonization remains controversial, and its effects on the genital tract are unknown. In this context, the present study aims to investigate the efficacy of vaccination in the prevention of experimental genital leptospirosis. (2) Methods: Eighteen sheep were divided into three groups: one vaccinated with a polyvalent commercial bacterin, one vaccinated with an autologous bacterin, and one unvaccinated. After 14 days, the sheep were experimentally challenged with 108 leptospires (L. santarosai, serogroup Sejroe, serovar Guaricura, strain FV52) intraperitoneally. Serology and colonization of the urinary and genital tracts were carried out monthly by PCR for 210 days in all animals. (3) Results: Vaccination significantly elicited antibody titers with a predominance of agglutinins against serogroup Sejroe. Both urinary and genital infections were confirmed by PCR. Positivity in urine PCR was absent after D120, while genital infection persisted until the end of the study. Serological results and the finding that vaccination did not avoid renal colonization align with previous studies. Despite vaccination, Leptospira established chronic and asymptomatic colonization of the genital tract until D210, an outstanding finding that remains to be fully understood in its mechanisms. (4) Conclusions: This is the first study conducted to analyze the effects of vaccination in the prevention of genital leptospirosis.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Efficacy of Vaccination in the Prevention of Renal and Genital Leptospirosis in Experimentally Infected Sheep

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Additionally, this serogroup has been associated with reproductive failures [3,17], which means that Sejroe strains play a key role in the reproductive syndrome of bovine genital leptospirosis (BGL). Despite its adaptation to bovine, the importance of these strains can be attributed to the preference of Sejroe for colonizing the uterus [18]. The main serovars of this group are Hardjoprajitno (L. interrogans) and Hardjobovis (L. borgpetersenii) [14].…”

Section: Agents and Epidemiological Aspectsmentioning

confidence: 99%

Bovine Genital Leptospirosis: An Update of This Important Reproductive Disease

Aymée,

Mendes,

Lilenbaum

2024

Animals

View full text Add to dashboard Cite

Bovine leptospirosis is an important disease that affects the reproductive sphere. Due to its high relevance for the bovine production chain in a worldwide scenario, a better understanding of the disease is crucial to reduce its negative impacts. The main agents are strains from the Sejroe serogroup, such as Hardjo and Guaricura, which lead to renal and genital infection. The genital colonization causes a chronic, silent, and subclinical reproductive syndrome, called Bovine Genital Leptospirosis (BGL). Embryonic death, estrus repetition, subfertility, and abortions are the main signs of BGL condition in females. However, although leptospires have been identified in semen, the manifestation of BGL in bulls remains to be clarified. The recommended diagnosis of BGL includes a serologic screening of the herds using the microscopic agglutination test followed by PCR of genital samples (cervicovaginal mucus, uterine fragment, or semen), especially from animals with reproductive failures. After the identification of carriers, control is carried out considering three steps: antimicrobial treatment of the carriers, environmental and reproductive management, and herd vaccination. Systematic testing, quarantine of newly arrived animals, and usage of antimicrobials in semen diluents or embryo culture media are other sanitary approaches that are encouraged to improve the control of the syndrome. Herein we discuss protocols for an efficient diagnosis and preventive procedures of BGL, which are fundamental to reducing the negative impact of the disease on cattle reproduction and its consequent economic hazards.

show abstract

“…Although both HP and HB are considered to be the major strains associated Editor's Choice with this reproductive leptospirosis (Nally et al 2018;Di Azevedo et al 2020;Loureiro and Lilenbaum 2020), strains belonging to Leptospira santarosai speciesand also from Sejroe serogroup (Guaricura genotypes)have been frequently recovered from Brazilian bovines (Hamond et al 2015;Loureiro et al 2016Loureiro et al , 2017. Additionally, when inoculated in hamsters, Guaricura strains have been strongly associated with uterine infection (Pinto et al 2020). Despite a high number of Guaricura isolates obtained from bovines, the problem has not yet been specifically associated with low reproductive efficiency (Loureiro et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Growth dynamic of Leptospira spp. from Sejroe serogroup in different media formulae

Loureiro

Brasil

Correia

et al. 2022

Letters in Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Significance and Impact of the Study:Obtaining pure cultures of local leptospires is currently a bottleneck in the understanding of epidemiology, pathogenesis, as well as the development of efficient vaccines. Worldwide cultivation techniques are applied based on human leptospirosis, which frequently inoculates blood and/or urine aseptic collected in Ellinghausen-McCullough-Johnson-Harris media. The present study, therefore, demonstrates that the failure to obtain pure leptospires cultures from bovine clinical samples should be associated with the chosen media. Commercially, this media has shown poor performance in culturing the main strains involved in bovine leptospirosis, whereas in-house media suggested by Ellis and associated with antimicrobial cocktails has proven to be a valuable choice for culturing leptospires from serogroup Sejroe.

show abstract

Short communication: Uterine leptospiral infection is strongly associated to strains of serogroup Sejroe on experimentally infected hamsters

Cited by 8 publications

References 27 publications

The Efficacy of Vaccination in the Prevention of Renal and Genital Leptospirosis in Experimentally Infected Sheep

The Efficacy of Vaccination in the Prevention of Renal and Genital Leptospirosis in Experimentally Infected Sheep

Bovine Genital Leptospirosis: An Update of This Important Reproductive Disease

Growth dynamic of Leptospira spp. from Sejroe serogroup in different media formulae

Contact Info

Product

Resources

About