Short communication: The effect of storage conditions and storage duration on milk ELISA results for pregnancy diagnosis

Wynands, Erin; LeBlanc, S.J.; Kelton, D.F.

doi:10.3168/jds.2017-12799

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…We observed increases of 0.1% (non-significant) and 0.4% percentage-points after freezing for 13–14 days and 25–28 days, respectively. Similar slight increase following storage at −20°C for 14 days and 28 days was observed on pregnancy-associated glycoproteins ELISA results for milk using multivariable analysis ( 12 ). Although, direct comparison between the two studies cannot be made, some of the storage temperatures and durations used in this study are comparable to the Wynands et al study.…”

Section: Discussionsupporting

confidence: 68%

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Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

Castonguay

Amine

et al. 2020

Front. Vet. Sci.

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An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions. The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK TM Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later. The third aliquots were either preserved for 13-14 days (n = 413) or 25-28 days (n = 432) at −20 • C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13-14 and 25-28 days-freezing. The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13-14 and 25-28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively. Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cutoff 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK TM has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.

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Section: Discussionsupporting

confidence: 68%

“…ELISA results for milk using multivariable analysis (12). Although, direct comparison between the two studies cannot be made, some of the storage temperatures and durations used in this study are comparable to the Wynands et al study.…”

Section: Discussionmentioning

confidence: 50%

Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

Castonguay

Amine

et al. 2020

Front. Vet. Sci.

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“…Były to próbki pobrane relatywnie wcześnie po inseminacji w okresie progowym -tj. takim, w którym zawartość PAG nie była jeszcze wystarczająca do postawienia jednoznacznej diagnozy (48).…”

Section: Tab 1 Wartości Parametrów Oceny Wiarygodności Diagnostycznej Wybranych Testów Do Wczesnego Wykrywania Ciążyunclassified

New possibilities for pregnancy diagnosis in ruminants: Limitations, advantages, use

Jaśkowski¹,

Herudzińska²,

Kmiecik³

et al. 2018

Medycyna Weterynaryjna

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Early pregnancy diagnosis in ruminants, especially in high production herds, is one of the most important components of reproduction management. Reducing the time between insemination and the exclusion or confirmation of pregnancy is of major economic importance. That is why the traditional method of pregnancy examination by transrectal palpation is increasingly complemented by additional tests, such as ultrasonography. An alternative to these methods of pregnancy diagnosis are also simple field tests. The best known are tests for progesterone and estrone sulphate concentrations. Over the last few years, the measuring of pregnancy-associated glycoproteins (PAGs) has also been gaining popularity. In cattle, this method can be used as early as the 28th day of pregnancy, when the plasma concentration of PAGs exceeds 1ng / ml. Maximum values of pregnancy-associated glycoproteins are observed about day 240 after fertilization. After calving, their level quickly decreases. In sheep, the concentration of PAGs increases significantly in the 3rd and 4th weeks of gestation and reaches its maximum level around the 9th week. In goats, the concentration of PAGs differs between races, but its measurement can be quite a reliable indirect method of pregnancy diagnosis. The PAGs value in this species grows until about the 8th week after insemination and, depending on the breed, reaches a maximum value of 50 to 69 ng/ml. One limitation for the use of PAGs field tests may be false positives which occur after the death of the embryo. Similarly, an increased PAGs concentration can persists for up to about 70 days after calving. Moreover, the PAGs concentration is also affected by the health, age and milk production of the female. The advantages of field tests are the simplicity of performance, the ease of reading and interpreting the results and the short time needed to complete the entire procedure. Field tests based on the commonly used ELISA method are characterized by low invasiveness (blood, milk), combined with high sensitivity and specificity. Their rapid application under field conditions makes them an excellent extension of diagnostic capabilities, especially in large herds of cows, .

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Short communication: The effect of storage conditions and storage duration on milk ELISA results for pregnancy diagnosis

Cited by 2 publications

References 13 publications

Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

New possibilities for pregnancy diagnosis in ruminants: Limitations, advantages, use

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