Short communication: Pheromonicin-SA affects mRNA expression of toll-like receptors, cytokines, and lactoferrin by Staphylococcus aureus-infected bovine mammary epithelial cells

Zhu, Yaohong; Liu, P.Q.; Weng, Xiaowen; Zhuge, Zengyu; Zhang, R.; Ma, Jiaze; Qiu, Xiao‐Qing; Li, R.Q.; Zhang, X.L.; Wang, J.F.

doi:10.3168/jds.2011-4703

Cited by 17 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Zhu et al (2012) when exposed primary mammary epithelial cells to Ph-SA found that this compound increases the expression of TLR2, TNF-α, IL-1β, IL-8 and lactoferrin and later the expression of TLR4. Thus, Ph-SA may be value as an antimicrobial in promoting innate immune response by S. aureus aureus-infected bovine mammary epithelial cells, especially regarding the inhibition of innate immune response induced by S. aureus which leads to the chronification of the inflammatory response (Bannerman et al, 2004;Lahouassa et al, 2007;Yang et al, 2008;Motshwene et al, 2009;Guntler et al, 2010;Lin et al, 2010;Lara-Zarate et al, 2011;Schukken et al, 2011;Wellnitz et al, 2012).…”

Section: Therapeutic Opportunitiesmentioning

confidence: 95%

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

Souza¹

2012

American Journal of Immunology

View full text Add to dashboard Cite

Mastitis is the most costly disease for dairy farmers and industry, which are mainly caused by the entry of bacteria to the teat canal. Shortly after the entry of the invading bacteria, the innate immunity recognizes the invading pathogen through pattern recognition receptors and initiates the inflammatory response necessary to eliminate the invading bacteria. This initial inflammatory response releases cytokines and chemoattractants for the rapid and massive influx of neutrophils from the blood to the site of infection which form the first line of cellular defense against bacteria This article reviewed the role of the most recent knowledge regarding the innate immunity in bovine mastitis focusing in the two major mastitis pathogens: Escherichia coli and Staphylococcus aureus the S. aureus appears to mostly circumvent the host immune response, as the Toll-Like Receptors (TLRs) signaling pathways. The Intramammary Infections (IMIs) by this bacteria result in a very moderate host response with minimal observable innate immune response, which are related to well-known ability to this pathogen to establish chronic IMI. Otherwise, E. coli elicits a strong and earlier response, mainly through TLR4, that is associated with the severity of the mastitis and the clinical manifestation commonly observed in dairy cows infected with this pathogen. Suboptimal and dysfunctional mammary defenses may contribute to the development of severe acute inflammation or chronic mastitis that adversely affects the milk production and quality. Thus, a better understanding of mastitis pathogen interaction to the host may be useful for future control of mastitis.

show abstract

Section: Therapeutic Opportunitiesmentioning

confidence: 95%

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

Souza¹

2012

American Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Primary BMECs were prepared from lactating cows as described previously (17), with some modifications. Briefly, mammary tissue pieces (0.5 to 1 mm 3 ) were transferred into 25-cm 2 cell culture flasks (Corning, Inc., Corning City, NY) and cultured in Dulbecco modified Eagle medium with Ham F-12 nutrient mixture (DMEM/F12; pH 7.2; Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (Gibco), 100 U of antibiotic (penicillin and streptomycin; Invitrogen, Carlsbad, CA)/ml, 5 g of bovine insulin/ml, 1 g of hydrocortisone/ml, 5 g of progesterone (I8405, H0888, and P8783, respectively; Sigma-Aldrich, St. Louis, MO)/ ml, and 10 ng of murine epithelial growth factor (PeproTech, Rocky Hill, NJ)/ml.…”

Section: Methodsmentioning

confidence: 99%

“…BMECs are poised to respond quickly to pathogen invasion by binding to pathogen-associated molecular pattern molecules via the activation of various pattern recognition receptors (PRRs) (16,17). In general, the binding of a pathogen to a Toll-like receptor (TLR) or nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) activates the MAPK and NF-B signaling cascades, leading to upregulate expression of various proinflammatory cytokines, chemokines, and antimicrobial peptides, thereby triggering inflammation and host immune responses (18).…”

mentioning

confidence: 99%

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation

Liu

Yang

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. We explored the effects of the probiotic Lactobacillus rhamnosus GR-1 on ameliorating E. coli-induced inflammation and cell damage in primary bovine mammary epithelial cells (BMECs). Increased Toll-like receptor 4 (TLR4), NOD1, and NOD2 mRNA expression was observed following E. coli challenge, but this increase was attenuated by L. rhamnosus GR-1 pretreatment. Immunofluorescence and Western blot analyses revealed that L. rhamnosus GR-1 pretreatment decreased the E. coli-induced increases in the expression of the NOD-like receptor family member pyrin domain-containing protein 3 (NLRP3) and the serine protease caspase 1. However, expression of the adaptor protein apoptosis-associated speck-like protein (ASC, encoded by the Pycard gene) was decreased during E. coli infection, even with L. rhamnosus GR-1 pretreatment. Pretreatment with L. rhamnosus GR-1 counteracted the E. coli-induced increases in interleukin-1␤ (IL-1␤), -6, -8, and -18 and tumor necrosis factor alpha mRNA expression but upregulated IL-10 mRNA expression. Our data indicate that L. rhamnosus GR-1 reduces the adhesion of E. coli to BMECs, subsequently ameliorating E. coli-induced disruption of cellular morphology and ultrastructure and limiting detrimental inflammatory responses, partly via promoting TLR2 and NOD1 synergism and attenuating ASC-independent NLRP3 inflammasome activation. Although the residual pathogenic activity of L. rhamnosus, the dosage regimen, and the means of probiotic supplementation in cattle remain undefined, our data enhance our understanding of the mechanism of action of this candidate probiotic, allowing for development of specific probiotic-based therapies and strategies for preventing pathogenic infection of the bovine mammary gland. Mastitis affects cows in all regions of the world and can cause a decrease in milk production and quality, resulting in a major economic burden to the dairy industry (1). Escherichia coli is by far the most common cause of mastitis, since it is isolated in more than 80% of cases of coliform mastitis (2). Antibiotic therapy frequently leaves residues in milk, potentially facilitating the development of antibiotic resistance. Probiotics represent a novel alternative to antibiotics for controlling pathogen infections.Lactobacillus rhamnosus GR-1 is a probiotic bacterium isolated from the female urethra, and oral ingestion of L. rhamnosus GR-1 and L. fermentum RC-14 reduces vaginal colonization by pathogenic bacteria and yeasts and maintains urogenital health in women (3). Pretreatment of pregnant CD-1 mice with L. rhamnosus GR-1 culture supernatant decreases lipopolysaccharide (LPS)-induced production of various cytokines and chemokines (4). Lactobacillus rhamnosus GR-1 suppresses expression of nuclear factor (NF)-B-related inflammatory genes and activates alternate mitogen-activated protein kinase (MAPK) and activator protein 1 pathways to recruit host defense factors...

show abstract

“…The content of IL-8 has been quantified to evaluate the effect of temperature on bioactive factors in milk (Ramírez-Santana et al, 2012). The mRNA expression of the IL-8 gene in primary bovine mammary epithelial cells as a marker of inflammation has been quantified to evaluate the antibiotic role of a newly developed, engineered multidomain peptide in promoting innate immune responses (Zhu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

IL-8 mRNA expression in the mouse mammary glands during pregnancy and lactation

Li¹,

Guo²,

Zhu³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. is an important inflammatory mediator. It is an angiogenic factor associated with inflammation and carcinogenesis. To date, research on IL-8 has been limited to its role as an indicator of inflammation. There has been no systematic research concerning IL-8 expression levels in the mouse mammary gland during pregnancy and lactation. Mouse mammary gland samples were collected on days 1, 6, 12, 18 of pregnancy and of lactation (6 mice per group). The expression levels of IL-8 mRNA were measured by semi-quantitative RT-PCR, with GAPDH as an internal control. IL-8 mRNA was highly expressed on day 1 of pregnancy in the mouse mammary glands (IL-8 IOD /GAPDH IOD = 1.68), and then suddenly declined; it reached 0.74 and 0.71 on days 6 and 12 of pregnancy. On day 18 of pregnancy, it started to increase (IL-8 IOD /GAPDH IOD = 1.02). However, the expression levels of IL-8 mRNA were not significant during pregnancy. During lactation, IL-8 expression level was lower than during pregnancy, but it stabilized at 0.32-0.41 (IL-8 IOD /GAPDH IOD ) from day 1 to day 18 of lactation, although the difference was not significant. We suggest that the changes in IL-8 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4746-4753 (2012) IL-8 mRNA expression in mouse mammary glands 4747 expression level during development is related to its regulatory role in mouse mammary gland immunity.

show abstract

Short communication: Pheromonicin-SA affects mRNA expression of toll-like receptors, cytokines, and lactoferrin by Staphylococcus aureus-infected bovine mammary epithelial cells

Cited by 17 publications

References 19 publications

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

The Innate Immunity in Bovine Mastitis: The Role of Pattern-Recognition Receptors

Lactobacillus rhamnosus GR-1 Ameliorates Escherichia coli-Induced Inflammation and Cell Damage via Attenuation of ASC-Independent NLRP3 Inflammasome Activation

IL-8 mRNA expression in the mouse mammary glands during pregnancy and lactation

Contact Info

Product

Resources

About