Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

Abstract

Abstract. Fauziah I, Asmara W, Wahyuni AETH, Widayanti R. 2021. Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia. Biodiversitas 22: 4890-4894. Infectious coryza (IC), caused by Avibacterium paragallinarum, is a contagious and infectious respiratory tract disease that affects the commercial poultry industry. Molecular techniques, such as species-specific PCR, HPG-2 PCR are mostly used for the detection of A. paragallinarum. The current research was carried out to isolate A. paragallinarum from the layers of infectious coryza signs in Sleman District, special region of Yogyakarta, followed by PCR confirmation of the identified bacteria. Nine field isolates were observed and determined based on their colony and cell morphology. All isolates were characterized biochemically and confirmed with species-specific HPG-2 PCR for A. paragallinarum. Out of 9 isolates, 6 (66.7%) isolates were biochemically identified as A. paragallinarum and confirmed by HPG-2 PCR.

Molecular detection and pyrG sequence analysis of Avibacterium paragallinarum using clinical samples of infraorbital exudates from layer chickens with infectious coryza symptoms in Indonesia

“…Isolation and identification of A. paragallinarum remain relatively difficult because the bacteria are fastidious and slow growing. Furthermore, since it requires specific growth factors [15,16], the cost of specialized media to culture and subsequently identify the bacteria is expensive [7]. Recently, the diagnostic options that can be used for IC have been expanded due to the development of species-specific PCR for Available at www.veterinaryworld.org/Vol.16/August-2023/10.pdf the molecular identification of A. paragallinarum [17,18].…”

“…[ 10 ] and Fauziah et al . [ 11 ] with some modifications to the PCR temperature. The primers used were F 5’- TGAGGGTAGTCTTGCACGCGAAT-3’ and R 3’-CAAGGTATCGATCGTCTCTCTACT-5’.…”

“…HPG-2 PCR, which targets a species-specific fragment of A. paragallinarum (~500 bp), was performed as described by Chen et al [10] and Fauziah et al [11] with some modifications to the PCR temperature. The primers used were F 5'-TGAGGGTAGTCTTGCACGCGAAT-3' and R 3'-CAAGGTATCGATCGTCTCTCTACT-5'.…”